Description

Book Synopsis


Table of Contents

PART ONE MICROSCOPY
  1. Use and Care of the Microscope
  2. Examination of Living Microorganisms
PART TWO HANDLING BACTERIA
  1. Microbes in the Environment
  2. Transfer of Bacteria: Aseptic Technique
PART THREE STAINING METHODS
  1. Preparation of Smears and Simple Staining
  2. Negative Staining
  3. Gram Staining
  4. Acid-Fast Staining
  5. Structural Stains (Endospore, Capsule, and Flagella)
  6. Morphological Unknown
PART FOUR CULTIVATION OF BACTERIA
  1. Isolation of Bacteria by Dilution Techniques
  2. Special Media for Isolating Bacteria
PART FIVE MICROBIAL METABOLISM
  1. Carbohydrate Catabolism
  2. Fermentation
  3. Protein Catabolism, Part 1
  4. Protein Catabolism, Part 2
  5. Respiration
  6. Unknown Identification and Bergey’s Manual
PART SIX MICROBIAL GROWTH
  1. Oxygen and the Growth of Bacteria
  2. Determination of a Bacterial Growth Curve: The Role of Temperature
  3. Biofilms
PART SEVEN CONTROL OF MICROBIAL GROWTH
  1. Physical Methods of Control: Heat
  2. Physical Methods of Control: Ultraviolet Radiation
  3. Chemical Methods of Control: Disinfectants and Antiseptics
  4. Chemical Methods of Control: Antimicrobial Drugs
  5. Effectiveness of Hand Scrubbing
PART EIGHT MICROBIAL GENETICS
  1. Regulation of Gene Expression
  2. Isolation of Bacterial Mutants
  3. Transformation of Bacteria
  4. DNA Fingerprinting
  5. Genetic Engineering
  6. Ames Test for Detecting Possible Chemical Carcinogens
PART NINE THE MICROBIAL WORLD
  1. Fungi: Yeasts and Molds
  2. Phototrophs: Algae and Cyanobacteria
  3. Protozoa
  4. Parasitic Helminths
PART TEN VIRUSES
  1. Isolation and Titration of Bacteriophages
  2. Plant Viruses
PART ELEVEN INTERACTION OF MICROBE AND HOST
  1. Epidemiology
  2. Koch's Postulate
PART TWELVE IMMUNOLOGY
  1. Innate Immunity
  2. Agglutination Reactions: Slide Agglutination
  3. Agglutination Reactions: Microtiter Agglutination
  4. ELISA Technique
PART THIRTEEN MICRORGANISMS AND DISEASE
  1. Bacteria of the Skin
  2. Bacteria of the Respiratory Tract
  3. Bacteria of the Mouth
  4. Bacteria of the Gastrointestinal Tract
  5. Bacteria of the Urogenital Trac
  6. Identification of an Unknown from a Clinical Sample
  7. Rapid Identification Methods
PART FOURTEEN MICROBIOLOGY AND THE ENVIRONMENT
  1. Microbes in Water: Multiple-Tube Technique
  2. Microbes in Water: Membrane Filter Technique
  3. Microbes in Food: Contamination
  4. Microbes Used in the Production of Foods
  5. Microbes in Soil: The Nitrogen and Sulfur Cycles
  6. Microbes in Soil: Bioremediation
APPENDICES.
  • A. Pipetting
  • B. Dilution Techniques and Calculations
  • C. Use of the Spectrophotometer
  • D. Graphing
  • E. Use of the Dissecting Microscope
  • F. Use of the Membrane Filter
  • G. Electrophoresis
  • H. Keys to Bacteria

Laboratory Experiments in Microbiology

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    A Spiral bound by Ted Johnson, Christine Case

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      View other formats and editions of Laboratory Experiments in Microbiology by Ted Johnson

      Publisher: Pearson Education (US)
      Publication Date: 08/01/2018
      ISBN13: 9780134605203, 978-0134605203
      ISBN10: 0134605209

      Description

      Book Synopsis


      Table of Contents

      PART ONE MICROSCOPY
      1. Use and Care of the Microscope
      2. Examination of Living Microorganisms
      PART TWO HANDLING BACTERIA
      1. Microbes in the Environment
      2. Transfer of Bacteria: Aseptic Technique
      PART THREE STAINING METHODS
      1. Preparation of Smears and Simple Staining
      2. Negative Staining
      3. Gram Staining
      4. Acid-Fast Staining
      5. Structural Stains (Endospore, Capsule, and Flagella)
      6. Morphological Unknown
      PART FOUR CULTIVATION OF BACTERIA
      1. Isolation of Bacteria by Dilution Techniques
      2. Special Media for Isolating Bacteria
      PART FIVE MICROBIAL METABOLISM
      1. Carbohydrate Catabolism
      2. Fermentation
      3. Protein Catabolism, Part 1
      4. Protein Catabolism, Part 2
      5. Respiration
      6. Unknown Identification and Bergey’s Manual
      PART SIX MICROBIAL GROWTH
      1. Oxygen and the Growth of Bacteria
      2. Determination of a Bacterial Growth Curve: The Role of Temperature
      3. Biofilms
      PART SEVEN CONTROL OF MICROBIAL GROWTH
      1. Physical Methods of Control: Heat
      2. Physical Methods of Control: Ultraviolet Radiation
      3. Chemical Methods of Control: Disinfectants and Antiseptics
      4. Chemical Methods of Control: Antimicrobial Drugs
      5. Effectiveness of Hand Scrubbing
      PART EIGHT MICROBIAL GENETICS
      1. Regulation of Gene Expression
      2. Isolation of Bacterial Mutants
      3. Transformation of Bacteria
      4. DNA Fingerprinting
      5. Genetic Engineering
      6. Ames Test for Detecting Possible Chemical Carcinogens
      PART NINE THE MICROBIAL WORLD
      1. Fungi: Yeasts and Molds
      2. Phototrophs: Algae and Cyanobacteria
      3. Protozoa
      4. Parasitic Helminths
      PART TEN VIRUSES
      1. Isolation and Titration of Bacteriophages
      2. Plant Viruses
      PART ELEVEN INTERACTION OF MICROBE AND HOST
      1. Epidemiology
      2. Koch's Postulate
      PART TWELVE IMMUNOLOGY
      1. Innate Immunity
      2. Agglutination Reactions: Slide Agglutination
      3. Agglutination Reactions: Microtiter Agglutination
      4. ELISA Technique
      PART THIRTEEN MICRORGANISMS AND DISEASE
      1. Bacteria of the Skin
      2. Bacteria of the Respiratory Tract
      3. Bacteria of the Mouth
      4. Bacteria of the Gastrointestinal Tract
      5. Bacteria of the Urogenital Trac
      6. Identification of an Unknown from a Clinical Sample
      7. Rapid Identification Methods
      PART FOURTEEN MICROBIOLOGY AND THE ENVIRONMENT
      1. Microbes in Water: Multiple-Tube Technique
      2. Microbes in Water: Membrane Filter Technique
      3. Microbes in Food: Contamination
      4. Microbes Used in the Production of Foods
      5. Microbes in Soil: The Nitrogen and Sulfur Cycles
      6. Microbes in Soil: Bioremediation
      APPENDICES.
      • A. Pipetting
      • B. Dilution Techniques and Calculations
      • C. Use of the Spectrophotometer
      • D. Graphing
      • E. Use of the Dissecting Microscope
      • F. Use of the Membrane Filter
      • G. Electrophoresis
      • H. Keys to Bacteria

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