Description

Book Synopsis
This book describes comprehensive step-by-step protocols for the delineation of genetic amplification and histological detection techniques. Each procedure has been tested and validated for its sensitivity, precision, and reproducibility, and the authors give advice on the design of primers for PCR applications and on optimizing these protocols for use with plant, insect, and prokaryotic cells.

Table of Contents
Overview;
Review of the PCR Technique;
Preliminary Solution-Based Reactions;
Preparation of Glass Slides and Tissues;
In Situ PCR: DNA and RNA Targets;
Special Applications of In Situ Amplification;
Hybridization Reactions;
Validation and Controls;
Materials and Methods;
Select Biography;
Appendices;
Index;
About the Authors.

InSitu PCR Techniques

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    A Paperback / softback by Omar Bagasra, John Hansen

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      Book details

      Publisher John Wiley & Sons Inc
      Published 18 July 1997
      ISBN-13 9780471159469
      978-0471159469
      ISBN-10 0471159468
      Also in:
      Biology, life sciences Computational biology / bioinformatics Genetics (non-medical)

      Description

      Book Synopsis
      This book describes comprehensive step-by-step protocols for the delineation of genetic amplification and histological detection techniques. Each procedure has been tested and validated for its sensitivity, precision, and reproducibility, and the authors give advice on the design of primers for PCR applications and on optimizing these protocols for use with plant, insect, and prokaryotic cells.

      Table of Contents
      Overview;
      Review of the PCR Technique;
      Preliminary Solution-Based Reactions;
      Preparation of Glass Slides and Tissues;
      In Situ PCR: DNA and RNA Targets;
      Special Applications of In Situ Amplification;
      Hybridization Reactions;
      Validation and Controls;
      Materials and Methods;
      Select Biography;
      Appendices;
      Index;
      About the Authors.

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