Description
Book SynopsisEnabling Technologies for the Dissection of Inositol Pyrophosphate Physiology.- Cell Extraction and Enrichment of Inositol Polyphosphate and Inositol Pyrophosphates Using Titanium Dioxide.- Analysis of Inositol Phosphates via Polyacrylamide Gel Electrophoresis (PAGE).- Enzymatic Synthesis of Inositol Pyrophosphates.- Determination of Steady-State Inositol Phosphate Profile of the Liverwort, Marchantia polymorpha Using SAX-HPLC.- Analysis of Inositol Phosphates, Diphosphoinositol Phosphates, and Their Analogues by HPLC on an Acid-Compatible Stationary Phase by Complexation with Ferric Ion.- Using HILIC-MS/MS Method to Study Inositol Pyrophosphate.- Capillary Electrophoresis Mass Spectrometry for Inositol (Pyro)Phosphate Profiling.- Absolute Configurations of Inositol Poly- and Pyrophosphates Assigned by Nuclear Magnetic Resonance Spectroscopy.- (PP)-InsP Affinity Probes for Target Characterization by Immunoblotting and Mass Spectrometry.- Enrichment Workflow for Pyrophosphoproteome Analysis.- The Use of Grating-Coupled Interferometry to Study Inositol Pyrophosphate-Protein Interactions.- Inositol Phosphate Kinase Architecture: Practical Approaches and Lessons Learned.- Visualizing and Identifying Inositol Pyrophosphate Isomers In Crystallo.- An In Vivo Yeast-Based Activity Assay for the PPIP5K Family.- Hydroponic Cultivation Systems to Study Nutrient-Dependent Changes in Inositol Polyphosphate Metabolism in Plants.- A Simple RUBY Reporter System in Nicotiana benthamiana for Studying Inositol Pyrophosphate Dynamics.- Knockout Mice as an Experimental Model to Study the Biology of Inositol Pyrophosphates.- IP6 Kinase Inhibitor Screening Assays.