Description
Book SynopsisChapters detail the assessment of macrophage activation, measuring innate immune responses to bacterial viability, quantification of secreted proteins, reporter systems, protocols examining specific innate immune activation by TLRs, RLRs, cGAS, and inflammasomes.
Table of Contents1 Emerging Concepts in Innate Immunity
Karin Pelka and Dominic De Nardo
2 Bioinformatic Assessment of Macrophage Activation by the Innate Immune System
Thomas Ulas, Joachim L. Schultze, and Marc Beyer
3 Generation of Genetic Knockouts in Myeloid Cell Lines using a Lentiviral CRISPR/Cas9 System
Paul J. Baker and Seth L. Masters
4 Modeling Primary Human Monocytes with the Trans-differentiation Cell Line BLaER1
Moritz M. Gaidt, Francesca Rapino, Thomas Graf, and Veit Hornung
5 Measurement of NF-κB Activation in TLR-activated Macrophages
Orna Ernst, Sharat J. Vayttaden, and Iain D.C. Fraser
6 Biochemical Isolation of the Myddosome from Murine Macrophages
Yunhao Tan and Jonathan C. Kagan
7 Generation of Innate Immune Reporter Cell Lines using Retroviral Transduction
Yamel Cardona Gloria and Dominic De Nardo
8 Myddosome Formation by Luminescence-based Mammalian Interactome Mapping (LUMIER)
Olaf-Oliver Wolz, Manfred Koegl, and Alexander N. R. Weber
9 Inflammatory Caspases: Activation and Cleavage of Gasdermin-D in vitro and during Pyroptosis
Yue Zhao, Jianjin Shi and Feng Shao
10 Methods for Detecting ASC and ASC Speck Formation
Florian Hoss, Verena Rolfes, Mariana R Davanso, Tarcio T Braga, and Bernardo S Franklin
11 Measuring Innate Immune Responses to Bacterial Viability
Julien Moretti, Nicolas Vabret, and J. Magarian Blander
12 Methods to Study Cell Swelling-induced Inflammasome Activation
Vincent Compan and Pablo Pelegrín
13 Detecting Release of Bacterial dsDNA into the Host Cytosol using Fluorescence Microscopy
Roland Felix Dreier, José Carlos Santos, and Petr Broz
14 Quantitative Proteomics of Secreted Proteins
Annika Frauenstein and Felix Meissner
15 Simultaneous Detection of Cellular Viability and Interleukin-1β Secretion from Single Cells by ELISpot
Stephanie A. Conos, Lisa M. Lindqvist, and James E. Vince
16 Detection and Quantification of MAV Aggregation via Confocal Imaging
Tan A Nguyen, Lachlan Whitehead, and Ken C Pang
