Description
Book SynopsisThis comprehensive and useful handbook represents a definitive up-to-date compendium of key in vitro bioassay methods that are employed to quantify and validate the anticancer activity of a drug candidate before it makes its way in to animal or clinical trials.
In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development covers the screening and evaluation of potential drug candidates in a wide category of anticancer assays demonstrating the specific ways in which various pharmaceutical bioassays interpret the activity of drug molecules. The major emphasis of the book is to present those bioassays which can be readily set up and practiced in any laboratory with limited funds, facilities or technical know-how.
Table of ContentsIntroduction to Anticancer Drug Development.
Cancer.
Chemotherapy.
Need for Effective Anticancer Drugs.
Stages of Anticancer Drug Development.
References.
In Vitro Biological Assays for Anticancer Chemotherapeutics.
Introduction.
Advantages of In Vitro Assays.
Disadvantages of In Vitro Assays.
Classification of In Vitro Assays.
Essential Techniques of Cell Cultures.
Primary Cell Cultures.
Maintenance of Cell Lines.
Subculture of Cells.
References.
Cell Viability and Cytotoxicity Assays.
Introduction.
Cell Membrane Integrity Assays.
Dye Exclusion Assays.
LDH Release Assays.
Functional or Metabolic Assays.
Tetrazolium Reduction Assays.
Sulforhodamine B Assay.
Acid Phosphatase Assay.
Alamar Blue Oxidation-Reduction Assay.
Neutral Red Assay.
Protease Viability Marker Assay.
Cell Adhesion Assay.
References.
Cell Proliferation Assays.
Introduction.
DNA Labeling Assays.
[3H]-Thymidine Assay.
BrdU Incorporation Assay.
Metabolic Assays.
Dye Assays.
ATP Assays.
Clonogenic Reproductive Assay.
References.
Apoptosis Assays.
Introduction.
Light Microscopy.
Western Blot Caspase Activation Assay.
Annexin V Ataining.
DNA Ladder Assay.
ELISA.
TUNEL Assay.
Comet Assay.
Acridine Orange/Ethidium Bromide Staining.
Mitochondrial Membrane Potential Assay.
Chromatin Condensation Assay.
APOPercentageTM Apoptotic Assay.
PARP Cleavage Assay.
Flow Cytometry.
References.
Cell Migratory Assays.
Introduction.
2D Cell Migration Assays.
Wound Healing Assay.
Cell Exclusion Zone Assay.
Transwell Migration Assay.
Fence Assay.
Microfluidic Assays.
Single Cell Motility Assay.
2D/3D Migration Assays.
Microcarrier Bead Assay.
Spheroid Migration Assay.
References.
In Vitro Cell Invasion Assays.
Introduction.
Transwell Invasion Assay.
Platypus Invasion Assay.
Gelatin Degradation Assay.
3D Cell Tracking Assay.
Vertical Gel 3D Invasion Assay.
Spheroid/Monodispersed Cell Invasion Assay.
Spheroid Confrontation Assay.
Spheroid Gel Invasion Assays.
References.
Anti-Angiogenesis Assays.
Introduction.
Endothelial Tube Formation Assay.
Rat Aortic Ring Assay.
References.
High-Throughput Screening Assays.
Introduction.
Cell Cultures.
High-Throughput Cytotoxicity and Apoptosis Screening in 2D Cultures.
3D Cell-Based HTS Assays.
References.
Index.
