Description
Book SynopsisThe past decade has seen tremendous advances in the study of G protein-coupled receptors (GPCRs), including the molecular cloning and identification of more than 100 hundred GPCR genes. But while GPCRs serve as targets for more than 300 medicines in the modern pharmacopoeia, the shrinking pool of known ligands and the continuing discovery of orphan GPCR genes have underscored the need for new approaches to ligand identification.
Identification and Expression of G Protein-Coupled Receptors addresses this new direction in GPCR biochemistry-offering a definitive laboratory bench manual that emphasizes expression over primary cloning strategies. In a series of expert contributions by well-known researchers, this book provides detailed protocols for various expression systems-from bacteria to mammalian cells-as well as straightforward opinions on the advantages and shortcomings of each approach. Topics covered include:
* Homology screening and the polymerase chain reaction in t
Table of ContentsCloning of G Protein-Coupled Receptor Genes: The Use of Homology Screening and the Polymerase Chain Reaction (A. Marchese, et al.).
Practical Approaches for Cloning G Protein-Coupled Receptors Using Mammalian Cell Expression (T. Murphy).
G Protein-Coupled Receptor Informatics and the Orphan Problem (K. Lynch).
The Use of Xenopus laevis Oocytes for the Study of G Protein-Coupled Receptors (A. Lee & M. Durieux).
Stable Expression of G Protein-Coupled Receptors in Mammalian Cells (K. Neve & R. Neve).
Heterologous Expression in Primary Cell Cultures (R. Leonard).
Expression of G Protein-Coupled Receptors in Escherichia coli (R. Grisshammer).
Large-Scale Expression/Purification of G Protein-Coupled Receptors Mammalian Cells (A. Robeva & J. Linden).
High-Level Expression of G Protein-Coupled Receptors in the Baculovirus/SF9 Cell Expression System (N. Rondeau, et al.).
Expression of G Protein-Coupled Receptors in Drosophila Schneider 2 Cells (M. Graziano, et al.).
Heterologous G Protein-Coupled Receptors Expressed in Saccharomyces cerevisiae: Methods for Genetic Analysis and Ligand Identification (M. Pausch, et al.).
Index.
