Description
Book SynopsisUnderstanding the major factors determining the specificity of transmembrane transfer processes in proteins is now a hot topic in molecular bio-science. Advanced electron paramagnetic resonance (EPR) at high magnetic fields is a powerful technique for characterizing the transient states of proteins in action. High-Field EPR Spectroscopy on Proteins and their Model Systems: characterization of Transient Paramagnetic States offers a comprehensive overview of experimental techniques in, and paradigmatic examples of, the application of high-field EPR spectroscopy in biology and chemistry. The book's focus is on the use of the technique in conjunction with site-specific mutation strategies and advanced quantum-chemical computation methods to reveal protein structure and dynamics. This yields new insights into biological processes at the atomic and molecular level. The theoretical and instrumental background of high-field EPR is described and examples of paradigmatic protein systems, such
Table of Contents
Introduction; Principles and illustrative examples of high-field/high-frequency EPR; Instrumentation; Computational; Methods for data interpretation; Conclusions and perspectives; Applications of high-field EPR to topical proteins and their model systems
