Description
Book SynopsisA huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to the usual molecular biology and proteomics methods, but reliable results can be obtained only if specific steps are followed with great care. This book provides detailed and precise guidelines for molecular analysis of archive tissues and will serve as an invaluable aid for researchers and pathologists involved in translational research and diagnostics. Clear notes and explanations are included to simplify use of the protocols for the less experienced. The authors are a group of acknowledged experts who have developed the described methods and validated them within the European project "Archive Tissues: Improving Molecular Medicine Research and Clinical Practice - IMPACTS", which has involved 21 leading institutions in 11 countries.
Trade ReviewFrom the reviews:
“This is a valuable resource that provides precise guidelines for molecular analysis of archive tissues essential for pathologists and researchers involved in translational research and diagnostics. … students, laboratory technicians, and physician-scientists interested in molecular analysis of archive tissues will find this book useful. … The clear explanations should enable even less experienced researchers to follow the protocols.” (Omer Iqbal, Doody’s Review Service, December, 2011)
Table of ContentsIntroduction.- Archive Tissues.- Pre-Analytical Treatment of Tissues.- Dissection of Tissue Components.- Extraction, Purification, Quantification and Quality Assessment of Nucleic Acids.- Reverse Transcription (RT).- Nucleic Acid Amplification.- DNA Sequencing.- Microsatellite Analysis.- Methylation Analysis.- Analysis of Copy Number Variations.- Microarrays.- Protein Extraction in Archive Tissues.- Protein Electrophoretic Separation.- Protein Immunodetection.- Mass Spectrometry.- Elements of Good Laboratory Practice..- DNA Sequencing.- Microsatellite Analysis.- Methylation Analysis.- Analysis of Copy Number Variations.- Microarrays.- Protein Extraction in Archive Tissues.- Protein Electrophoretic Separation.- Protein Immunodetection.- Mass Spectrometry.- Elements of Good Laboratory Practice.
