Description
Book SynopsisThis current edition explores new tests for genotoxicity testing, along with other well-known techniques. This will further help in our understanding of the genotoxic effects of chemicals. The book has different sections dealing with various assays for gene mutation, chromosomal abnormalities, primary DNA damage, etc. It also delves into plant models, animals and their alternates, as well as
in silico approaches for genetic toxicology. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Genotoxicity Assessment: Methods and Protocols, Second Edition serves as a highly useful and ready resource for research students and scientists working in regulatory toxicology as well as biomedical, biochemical, and pharmaceutical sciences.
Table of ContentsPart I: Gene Mutation Assays
1. In Vitro Mouse Lymphoma Cell (L5178Y Tk+/- -3.7.2.C) Forward Mutation Assay
Melissa R. Schisler, Martha M. Moore, and B. Bhaskar Gollapudi
2. Erythrocyte-Based Pig-a Gene Mutation Assay
Jeffrey C. Bemis, Nikki E. Hall, and Stephen D. Dertinger
3. Detection of In Vivo Mutation in the Hprt and Pig-a Genes of Rat Lymphocytes
Vasily N. Dobrovolsky, Joseph G. Shaddock, Roberta A. Mittelstaedt, Daishiro Miura, and Robert H. Heflich
Part II: Assays for Chromosomal Abnormalities
4. In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests
Pasquale Mosesso and Serena Cinelli
5. Dicentric Chromosome Assay (DCA) and Cytokinesis-Block Micronucleus (CBMN) Assay in the Field of Biological Dosimetry
Antonella Testa, Valentina Palma, and Clarice Patrono
6. Chromosomal Aberration Test in Human Lymphocytes
Christian Johannes and Guenter Obe
7. In Vivo Micronucleus Assay in Mouse Bone Marrow
Abhishek Kumar Jain and Alok Kumar Pandey
8. Micronucleus Cytome Assays in Human Lymphocytes and Buccal Cells
Claudia Bolognesi and Michael Fenech
9. Micronucleus Analysis by Flow Cytometry
Azeddine Elhajouji and Pascal Stadelmann
10. Hen’s Egg Test for Micronucleus Induction (HET-MN)
Kerstin Reisinger, Eva Dony, Thorsten Wolf, and Katrin Maul
11. Comparative Genomic Hybridization (CGH) in Genotoxicology
Adi Baumgartner, Veronika Hartleb, and Jim D. Taylor
Part III: Tests for Primary DNA Damage
12. The Comet Assay: Assessment of In Vitro and In Vivo DNA Damage
Mahima Bajpayee, Ashutosh Kumar, and Alok Dhawan
13. The Comet Assay in Human Biomonitoring
Diana Anderson and Julian Laubenthal
14. The Comet Assay in Marine Animals
Patrizia Guidi, Brett P. Lyons, and Giada Frenzilli
15. Multicolour Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers
Julian Laubenthal, Michal R. Gdula, Alok Dhawan, and Diana Anderson
16. Detection of DNA Double Strand Breaks Using Pulse Field Gel Electrophoresis
Vyom Sharma and Vishwa Mohan
17. Detection of UV-Induced Thymine Dimers
Vipin Kumar Yadav, Poorwa Awasthi, and Amit Kumar
Part IV: Assays in Plants and Alternate Animal Models
18. Micronucleus Assay with Tetrad Cells of Tradescantia
Miroslav Mišík, Clemens Pichler, Bernhard Rainer, Armen Nersesyan, Katarina Mišíková, and Siegfried Knasmueller
19. The Wing-Spot and the Comet Tests as Useful Assays for Detecting Genotoxicity in Drosophila
Ricard Marcos and Erico R. Carmona
Part V: In Silico Testing for Genetic Toxicology
20. In Silico Approaches in Predictive Genetic Toxicology
Meetali Sinha, Alok Dhawan, and Ramkrishnan Parthasarathi
