Description
Book SynopsisThis advanced textbook teaches the theory and practice of molecular genetic analysis to senior undergraduates and graduates studying genetics, molecular biology and cell biology. It uses a case study approach, with the yeast Saccharomyces as the model genetic organism, to explain the theory and practice of molecular genetic analysis.
Trade Review"...Students new to yeast genetics and those of us keeping abreast of fusing disciplines should add this publication to our reading list..." (The Biochemist, 4 October 2002)
"... useful addition to the literature..." (Biologist, Vol.50. No.2, 2003)
"...a valuable addition to the reference library of any research laboratory at universities, research institutes and the industry." (Acta Biotechnologica, Vol.23, No.2-3, 2003)
"...The aim of every teacher is to inspire students to move down from the stands, step up to the plate, and start hitting on their own. This book is a wonderful tool to help in that quest." (Cell Biology Education, 16 June 2003)
Table of ContentsIntroduction.
SECTION I: SACCHAROMYCES CEREVISIAE AS A GENETIC RESEARCH ORGANISM.
Saccharomyces cerevisiae As a Genetic Model Organism
Techniques in Cell and Molecular Biology
Saccharomyces Cell Structure.
SECTION II: TECHNIQUES OF GENETIC ANALYSIS.
Mutant Hunts - To Select or to Screen (perhaps even by brute force).
Complementation Analysis: How many Genes are Involved?
Epistasis Analysis.
Gene Isolation and Analysis of Multiple Mutant Alleles.
Suppression Analysis.
Enhancement and Synthetic Phenotypes
Two-hybrid Analysis
Advanced Concepts in Molecular Genetic Analysis
Genomic Analysis
SECTION III: CASE STUDIES FROM THE SACCHAROMYCES GENETIC LITERATURE.
Case Study I: Glucose Sensing and signaling Mechanisms in Saccharomyces.
Case Study II: Secretion, Exocytosis and Vesicle Trafficking in Saccharomyces.
Case Study III: The Cell Division Cycle of Saccharomyces.
Case Study IV: Mating-type Pheromone Response Pathway of Saccharomyces.
Index.
