Description
Book SynopsisMolecular Genetic Analysis is an advanced textbook to teach the theory and practice of molecular genetic analysis to senior undergraduates and graduates studying genetics, molecular biology and cell biology.
This book uses a case study approach, with the yeast Saccharomyces as the model genetic organism, to explain the theory and practice of molecular genetic analysis. It provides enough information so readers will be able to apply the approach to their own research project.
Trade Review"...Students new to yeast genetics and those of us keeping abreast of fusing disciplines should add this publication to our reading list..." (The Biochemist, 4 October 2002)
"... useful addition to the literature..." (Biologist, Vol.50. No.2, 2003)
"...a valuable addition to the reference library of any research laboratory at universities, research institutes and the industry." (Acta Biotechnologica, Vol.23, No.2-3, 2003)
"...The aim of every teacher is to inspire students to move down from the stands, step up to the plate, and start hitting on their own. This book is a wonderful tool to help in that quest." (Cell Biology Education, 16 June 2003)
Table of ContentsIntroduction.
SECTION I: SACCHAROMYCES CEREVISIAE AS A GENETIC RESEARCH ORGANISM
1. Saccharomyces cerevisiae As a Genetic Model Organism
2. Techniques in Cell and Molecular Biology
3. Saccharomyces Cell Structure
SECTION II: TECHNIQUES OF GENETIC ANALYSIS
4. Mutant Hunts - To select or to screen (perhaps even by brute force)
5. Complementation analysis: How many genes are involved?
6. Epistasis analysis
7. Gene isolation and analysis of multiple mutant alleles
8. Suppression Analysis
9. Enhancement and synthetic phenotypes
10. Two-hybrid analysis
11. Advanced concepts in molecular genetic analysis
12. Genomic Analysis
SECTION III: CASE STUDIES FROM THE SACCHAROMYCES GENETIC LITERATURE
Case Study I: Glucose Sensing and Signaling mechanisms in Saccharomyces
Case Study II: Secretion, Exocytosis and Vesicle Trafficking in Saccharomyces
Case Study III: The Cell Division Cycle of Saccharomyces
Case Study IV: Mating-type Pheromone Response Pathway of Saccharomyces
Index.
