Description
Book SynopsisProvides a comprehensive guide to the use of gas chromatographymass spectrometry (GC-MS) on environmentally significant organic compounds
This book presents a library of mass spectra of 1,725 biologically and environmentally important organic compounds, in the form of their trimethylsilyl derivatives (TMS), as well as their linear temperature programmed chromatographic retention indices, RI, whose values are in the range of 700-4700 index units. Of the compounds presented, more than 60% of compounds have not previously been characterized by their mass spectra, and more than 70% not previously been characterized by their RI values. Some of these compounds, never before analysed via MS and GC, were detected by the author's team in plant tissues.
The first chapters of the book are devoted to the methodology and practice of sample preparation, as well as to mass spectrometry considerations. They contain the discussion of possible complica
Table of Contents
Preface vii
1 Introduction 1
2 Gas Chromatography and Mass Spectrometry Considerations 7
2.1 Silylation Reagents and Procedure of Derivatisation 7
2.2 Two-Step Derivatisation: Oximating−Silylating 8
2.3 Analytical Procedure 10
2.3.1 Selection of Stationary Phase and Conditions of Separation 10
2.3.2 Sample Preparation 10
2.3.3 Qualitative MS Identification 12
2.3.3.1 Aliphatic Alcohols 16
2.3.3.2 Aromatic Alcohols 16
2.3.3.3 Carboxylic Acids 16
2.3.3.4 Amino Acids 16
2.3.3.5 Phenylpropanoids 17
2.3.3.6 Flavonoids 18
2.3.3.7 Steroids 20
2.3.3.8 Carbohydrates and Glycosides 22
2.4 A Critical Role of Retention Indices in GS–MS Investigations 24
2.5 Experimental Factors Influencing the Accuracy of Retention Indices 28
2.6 Possible Artefacts and Complications of Mass Spectral Identification 30
3 Explanation of Format Used in the Book 33
4 References 35
5 Alphabetic Listing of Compounds 43
6 Compounds Listed in Order of Their Retention Indices 87
7 Mass Spectra and Retention Indices of TMS Derivatives 131
