Description
Book SynopsisProteomics aims to study all the proteins of human and other living systems, as well as their properties to provide an integrated view of cellular processes. The study of proteomics involves the application of rapidly evolving high-throughput technologies and new platforms that are coming forward regularly, providing versatile novel tools for biomedical and pharmaceutical applications.
This book provides a detailed understanding of the basics of proteins and proteomics, gel based-proteomics techniques, basics of mass spectrometry and quantitative proteomics, interactomics: basics and applications, and advancements in proteomics. It also covers basic knowledge about sample preparation, mass spectrometry workflow, different chromatography technologies and quantitative proteomics.
The text highlights the application and challenges of various high-throughput integrated proteomics technologies capable of fast and accurate screening of thousands of biomolecules, which are fou
Table of Contents
Module 1: Basics of proteins and proteomics
Basics of amino acids and proteins
Protein chemistry to proteomics
Module 2: Gel-based proteomics techniques
Gel-based proteomics
2-d difference in gel electrophoresis (2d-dige)
Gel-based proteomic data analysis
Module 3: Basics of mass-spectrometry and quantitative proteomics
Introduction to mass spectrometry
Hybrid mass spectrometry configurations
Tandem mass spectrometry for protein identification
In vitro quantitative proteomics using itraq
In vivo quantitative proteomics using silac
Module 4: Interactomics: basics and application
Introduction to interactomics
Antigen and antibody microarrays
Cell-free expression based protein microarrays
Nucleic acid programmable protein arrays
Label-free proteomics
Surface plasmon resonance
Surface plasmon resonance imaging
Protein interaction analysis using spr and spri
Module 5: Advancement in proteomics
Proteomics for translational research
Future of proteomics for clinical applications
Challenges in clinical proteomics
