Description
Book SynopsisBreaking Barriers: A Protocol to Investigate Intestinal Pathogen Adhesion, Invasion and Translocation Through In Vitro, In Vivo, and Imaging Technologies.- Quantitative assessment of MUC5AC and Helicobacter pylori adhesion to the gastric tissue on the model of Cavia porcellus.- Growth and Polarization of A549 Human Alveolar Epithelial Cells as a Model to Study Biofilm Formation in Acinetobacter baumannii.- Mucins as inhibitors against bacterial adhesion to epithelial cells.- Profiling of anti-glycan IgA and IgG in oral fluid using a glycan microarray.- Assays for Anti-Helicobacter pylori Effects of Lactic Acid Bacteria Secretions.- Assessing the relative affinities of bacterial lectins for sugars.- Investigating bacterial invasion of highly differentiated human bronchial epithelial cell barriers.- Methods of a reproducible mouse model of Clostridioides difficile infection to investigate novel bacterial based therapies.- Purification of Type IV and Type VI lipoteichoic acids from Gram-positive mucosal pathogens via a two-step hot water-phenol/triethylammonium acetate extraction method.- Microbial lectins targeting mucus: A glycobioinformatic approach.- Exploring the O-glycomic Degradome Using Natural Mucin Libraries.- Detection of Bacterial Adhesion to Mucus Slot-Blotted on Polyvinylidene Difluoride (PVDF) Membrane.- Quantification of β-Lactamase Inhibition using a Luminescent Whole-Cell Biosensor.- Mucins and bacterial interactions.- Measuring and Analyzing Bacterial Movement in Mucus.- Adhesion of Pseudomonas aeruginosa to human outer layer airway mucins.
