Description
Book SynopsisThis third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and cell lineage tracing, protein inhibition and tagging, optogenetic and optochemical control of tissue mechanics, AFM measurements, sample preparation for electron microscopy, live imaging of different tissues and organs, and quantitative image analysis. Written in the format of the highly successful Methods in Molecular Biology series, chapters include an introduction to their topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfallsAuthoritative and cutting-edge,Drosophila: M
Table of Contents
1. Using FlyBase, a database of Drosophila genes and genetics
Victoria K. Jenkins, Aoife Larkin, Jim Thurmond and The FlyBase Consortium
2. The Q-System: A versatile repressible binary expression system
Orsolya Fölsz, Chun-Chieh Lin, Darya Task, Olena Riabinina and Christopher J. Potter
3. Resources and methods for the analysis of microRNA function in Drosophila
Sromana Mukherjee and Nicholas Sokol
4. Analysis of single-cell transcriptome data in Drosophila
Schayan Yousefian, Maria Jelena Musillo and Josephine Bageritz
5. Prime editing for precise genome engineering in Drosophila
Justin A. Bosch and Norbert Perrimon
6. CRISPR/Cas9 mediated precise and efficient genome editing in Drosophila
Kevin G. Nyberg and Richard W. Carthew
7. Tissue-specific CRISPR-Cas9 screening in Drosophila
Fillip Port and Michael Boutros
8. CRISPR-based transcriptional activation in Drosophila
Yuting Han, Xinyi Lu, Yutong Li, Yuhao Qiu, Xizhi Dong, Xiaochen Li, Xu Si, Qingfei Liu, Jian-Quan Ni
9. Tracing and manipulating
Drosophila cell lineages based on CRISPR: CaSSA and CLADES
Jorge Garcia-Marques and Tzumin Lee
10. Studying protein function using nanobodies and other protein binders in Drosophila
Katarzyna Lepeta, Milena Bauer, Gustavo Aguilar, Alessandra Vigano, Shinya Matsuda and Markus Affolter
11. Anchor Away: A system for fast inhibition of proteins in Drosophila
Pablo Sanchez Bosch
12. Tagging Drosophila proteins with genetically-encoded fluorophores
Jerome Avellaneda and Frank Schnorrer
13. Optogenetic methods to control tissue mechanics in Drosophila
Daniel Krueger and Stefano De Renzis
14. Optochemical control of cell contractility in Drosophila embryos
Deqing Kong and Jörg Großhans
15. Stiffness measurement of Drosophila egg chambers by atomic force microscopy
Uwe Töpfer, Karla Yanín Guerra Santillán and Elisabeth Fischer-Friedrich
16. Cultivation and live imaging of Drosophila imaginal discs
Natalie A. Dye
17. Sample preparation and imaging of the pupal Drosophila abdominal epidermis
Daiki Umetsu
18. FlyClear: A tissue clearing technique for high-resolution microscopy of Drosophila
Marko Pende, Saiedeh Saghafi, Klaus Becker, Thomas Hummel and Hans-Ulrich Dodt
19. Preparation of Drosophila tissues and organs for transmission electron microscopy
Olympia-Ekaterini Psathaki and Achim Paululat
20. Segmentation and quantitative analysis of epithelial tissues
Benoit Aigouy and Benjamin Prud’homme
21. Genetically-encoded sensors to study metabolism in Drosophila
Ellen McMullen, Helen Hertenstein, Stephan Müller and Stefanie Schirmeier
