Description
Book SynopsisThere is a growing interest in the use of nanoparticles modified with DNAs, viruses, peptides and proteins for the rational design of nanostructured functional materials and their use in biosensor applications. The challenge is to control the organization of biomolecules on nanoparticles while retaining their biological activity as potential chemical and gene therapeutics. These noble metal nanoparticles/biomolecules conjugates have specific properties and therefore they are attractive materials for nanotechnology in biochemistry and medicine.
In this book, the author review work performed dealing with the DNA structure and functionalities, interactions between DNA, noble metal nanoparticles, surface active agents, solvents and other additives. Particular attention is given to how the DNA''s chain length and the DNA conformation affect the interaction and structure of the nanoconjugates and nanostructures that are formed. Also discussed are the recent advances in the preparat
Table of Contents
Acknowledgement ix
Preface xi
1 Introduction 1
2 Nucleic Acids 29
2.1 DNA/RNA Basics 29
2.2 Aptamers, Telomers and Oligonucleotides 50
2.3 Techniques and Approaches 63
2.4 DNA-Based Molecular Nanomachines 90
2.5 Peptide Nucleic Acid 104
2.6 Nanobiotechnology 112
3 Noble Metal Nanoparticles 121
3.1 Preparation and Modification 121
3.2 Optical and Physical Properties 137
3.3 Conjugates 144
4 DNA-Based Conjugates 149
4.1 General 149
4.2 Condensation 152
4.3 Conjugates 170
5 DNA-Noble Metal Nanoparticle Conjugates 173
5.1 General Approaches 173
5.2 DNA Monomers and Oligomers 178
5.2.1 Gold Nanoparticles 178
5.2.2 Silver Nanoparticles 207
5.3 Hybridization and Denaturation 224
5.4 DNA Biotemplates 273
6 DNA-Gold Nanoparticle Conjugates 281
6.1 DNA-Gold Zero-Dimensional Nanoparticle Conjugates 281
6.2 DNA One-Dimensional Gold Nanoparticle Conjugates 295
7 PNA-Noble Metal Nanoparticles Conjugates 315
7.1 PNA-Gold Nanoparticle Conjugates 315
7.2 PNA-Silver Nanoparticle Conjugates 328
8 DNA-Silver Nanoparticles Conjugates 335
9 The Structure of DNA-Noble Metal Nanoparticles Conjugates 345
9.1 Configuration of DNA-Noble Metal Nanoparticles Conjugates 345
9.2 Stabilization of DNA Conjugates 358
9.3 Nanostructures and Nanoconstructs 369
9.4 Colorimetric and Sensing Assays 385
10 Photochemical and Photophysical Events 403
10.1 Noble Metal Nanoparticles 403
10.2 DNA Nucleobases 406
10.3 DNA/PNA 417
10.4 DNA-Dyes Conjugates 429
10.5 DNA-AuNP-Dye Conjugates 443
10.6 DNA-Gold Nanoparticle Conjugates 455
10.7 DNA-AgNPs 465
10.8 Hot Gold Nanoparticles 475
11 Nanoparticle Therapeutics 481
11.1 Biodecorated Nanoparticle-Based Therapies 481
11.2 Photothermal Therapy 489
11.3 Cells 495
11.4 Gene Therapy 499
11.5 Blood Fluid Effect 505
11.6 Other Application Approaches 507
12 Conclusion 515
Nomenclature 533
DNA Entities 545
Vocabulary and Definitions 549
References 564
Index
