Description
Book SynopsisThis volume provides detailed methods and key approaches used to mechanistically study DNA damage, as well as the factors involved in the damage response.
Table of Contents1.Robust Computational Approaches to Defining Insights on the Interface of DNA Repair with Replication and Transcription in Cancer
Albino Bacolla and John A. Tainer
2. A Whole Genome CRISPR/Cas9 Screening Approach for Identifying Genes Encoding DNA End-Processing Proteins
Bo-Ruei Chen and Barry P. Sleckman
3.Immunoaffinity Purification of Epitope-Tagged DNA Repair Complexes from Human Cells
Brittany A. Townley, Jennifer M. Soll, and Nima Mosammaparast
4.Universally Accessible Structural Data on Macromolecular Conformation, Assembly, and Dynamics by Small Angle X-Ray Scattering for DNA Repair Insights
Naga Babu Chinnam, Aleem Syed, Kathryn Burnett, Greg Hura, John A. Tainer, and Susan E. Tsutakawa
5.Assessing DNA damage responses using B lymphocyte cultures.
Rachel Johnston, Lynn S. White, and Jeffrey J. Bednarski
6.Studying single stranded DNA gaps at replication intermediates by electron microscopy
Jessica Jackson and Alessandro Vindigni
7.Approaches to monitor termination of DNA replication using Xenopus egg extracts
Tamar Kavlashvili and James M. Dewar
8.Use of high-performance liquid chromatography coupled to mass spectrometry to quantify modified nucleosides
Rebecca Rodell, Adit Ganguly, Ning Tsao, and Nima Mosammaparast
9. Targeted Formation of 8-oxoguanine in Telomeres
Ryan P. Barnes, Sanjana A. Thosar, Elise Fouquerel, and Patricia L. Opresko
10. Qualitative and Quantitative Analysis of DNA-Cytidine Deaminase Activity
Rachel DeWeerd and Abby M. Green
11. Characterization of DNA-PK-bound end fragments using GLASS-ChIP
Rajashree A. Deshpande and Tanya T. Paull
12. Monitoring Nuclease Activity by X-ray Scattering Interferometry using Gold Nanoparticle Conjugated DNA
Daniel J. Rosenberg, Aleem Syed, John A. Tainer, and Greg L. Hura
13. In Vitro Reconstitution of BRCA1-BARD1/RAD51 Mediated Homologous DNA Pairing
Meiling Wang, Cody M. Rogers, Dauren Alimbetov, and Weixing Zhao
14. Purification of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) from
HeLa cells
Linda Lee, Yaping Yu, and Susan P. Lees-Miller
15. Purification and characterization of human DNA ligase III complexes after expression in insect cells
Ishtiaque Rashid, Miaw-Sheue Tsai, Aleksandr Sverzhinsky, Aye Su Hlaing, Brian Shih, Aye C. Thwin, Judy G. Lin, Su S. Maw, John M. Pascal, and Alan E. Tomkinson
16. Generation of monoubiquitin and K63-linked polyubiquitin chains for protein interaction studies
Rita Anoh, Kate A. Burke, Dhane P. Schmelyun, and Patrick M. Lombardi
