Description
Book SynopsisThis thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.
Table of Contents1-Basic idea about nucleic acids for non-experts
2-General steps during isolation of DNA and RNA
3-Phenol-chloroform DNA isolation method
4-RNA isolation by the guanidinium-acid-phenol method
5-Spin column-based isolation of nucleic acid
6-Isolation of plasmid DNA by alkaline lysis
7-DNA Isolation by Hydrophilic Ionic Liquid Treatment
8-Lithium Chloride based isolation of DNA and RNA
9-Isolation of Bacteriophage DNA by PEG Method
10-DNA isolation by Chelex Method
11-DNA isolation by Lysozyme and Proteinase K
12-Salting-out method for blood samples
13-CTAB or SDS based isolation of plant’s DNA
14-DNA extraction by spooling method
15-Magnetic bead-based nucleic acid isolation
16-Density gradient-based nucleic acid isolation
17-DNA extraction from agarose gel though paper-strips
18-TRANSFORMATION OR GENETIC MODIFICATION OF CELLS/ORGANISMS
19-GENE CLONING AND VECTORS
20-POLYMERASE CHAIN REACTION (PCR)
21-Southern and Northern Blotting
22-GENOME MAPPING
23-Applications of DNA Sequencing Technologies for Current Research
