Description
Book SynopsisA complete account of the theory of the diffraction of X-rays by crystals, with particular reference to the processes of determining the structures of protein molecules. This book is aimed primarily at structural biologists and biochemists but will also be valuable to those entering the field with a background in physical sciences or chemistry. It may be used at any post-school level, and develops from first principles all relevant mathematics, diffraction and wave theory, assuming no mathematical knowledge beyond integral calculus. The book covers a host of important topics in the area, including:- The practical aspects of sample preparation and X-ray data collection, using both laboratory and synchrotron sources - Data analysis at both theoretical and practical levels- The important role played by the Patterson function in structure analysis, by both molecular replacement and experimental phasing approaches - Methods for improving the resulting electron density map - The theoretical
Trade ReviewThe first two-thirds of this book was like a thriller to me. Even though I knew the answer, I wanted to see how the author would address the next topic and I could not put it down. * Joseph D. Ferrara, Ph.D, Crystallography Times *
This is one of the best crystallography books ever written, and it is with pleasure that I wholeheartedly recommend it. * Nicholas M. Glykos, Democritus University of Thrace, Greece *
The authors have nicely brought the bibliography up to date and mention recent method developments, giving a good first grasp of what is involved in solving a structure. The text also makes good use of accompanying, illustrative figures, which is most essential when developing the complex concepts of diffraction, Fourier transformation and convolution. * E. von Castelmur and A. Perrakis, Crystallography Reviews *
In my opinion, this book would be the perfect textbook for a theoretical course on macromolecular crystallography * Manfred S. Weiss, Acta Crystallographica Section D *
A welcome addition to any structural biology laboratory, [and] an invaluable reference, answering questions in an accurate and transparent manner * Karen McLuskey, Chemistry World *
Table of ContentsPART I: FUNDAMENTALS; PART II: DIFFRACTION THEORY; PART III: STRUCTURE SOLUTION
