Description
Book SynopsisThe vast number of chemicals existing or being added into the environment, have globally aroused great concern regarding their adverse effects in human population. Development and validation of sensitive and better test systems which can assess the adverse effects of chemicals at an early stage for intervention strategies to be implemented in time is currently in progress. This book documents the latest research and showcases the versatile, state-of-the-art technique - the Comet assay - in the field of modern toxicology. The assay is a simple, sensitive rapid and visual technique for the quantitative and qualitative assessment of DNA damage in single cells. The Comet Assay in Toxicology is the first book of its kind to be devoted exclusively to the Comet assay and its applications as an important tool in modern toxicology. This multi-author book will serve as both a reference and a guide to investigations in the biomedical, biochemical and pharmaceutical sciences. Written by investigat
Table of ContentsPreface; Part I: Genesis of Comet assay; Part II: Various procedures for the Comet assay; Oxidative damage; Water disinfection by-products; Comet assay in Plants; Double Strand; Breaks in bacteria; Comet - FISH; Double strand breaks (DSBs) - radiation/challenge assay - Radiation; Apoptosis; Multiple mouse organs; Part III: Applications of Comet assay; Dietary intervention studies; Comet assay in Mussels; Exercise; Arsenic; DSBs - radiation/challenge assay - Radiation; In vivo Comet assay; In vitro parallelogram approaches; Photobiology; Comet assay in sperm; Comet assay in Human monitoring; Part IV: Guidelines for comet assay; In Vitro And In Vivo Guidelines for the Comet Assay; In vivo Guidelines for Comet; Part V: Image analysis and Statistics; Models for image analysis; Statistics in comet assay
