Description
Book SynopsisTable of Contents1. The challenge of dissecting gene function in model organisms: tools to characterize genetic mutants and assess transcriptional adaptation in zebrafish Magdalena Cardenas Rodriguez and Iain Drummond 2. Human LUHMES and NES cells as models for studying primary cilia in neurons Peter Swoboda 3. Fixation methods and immunolabeling for cilia proteins in ciliary and extraciliary locations Russell J. Ferland 4. Single-molecule imaging in the primary cilium Lucien E. Weiss 5. Methods to analyze primary cilia in mouse cardiac lesion model Alfredo Criollo Sr., Daniel Peña-Oyarzun, Rodrigo Troncoso, Joseph A. Hill, Sergio Lavandero and Mauricio Budini Sr. 6. Methods to study motile ciliated cell types in a zebrafish brain mode Nathalie Jurisch-Yaksi 7. Clinical and Molecular diagnosis in BBS (Bardet-Biedl syndrome) Diana Valverde 8. Modelling ciliopathies in patient-derived primary cells Markus Schueler and Kai-Uwe Eckardt 9. Analysis of motility and mucociliary function of tracheal epithelial cilia Koji Ikegami 10. High-speed Super-resolution SPEED Microscopy to study Live Primary Cilium Weidong Yang 11. Method for siRNA delivery in retina explants Florent Poulhès 12. Methods to study primary cilia and autophagy in the brain Olatz Pampliega 13. Multi-color live-cell fluorescence imaging of primary ciliary membrane assembly dynamics Christopher Westlake
