Description
Book SynopsisThis text provides students with the information they need to know about how to make chemical measurements in biological systems. The emphasis is on the measurement process rather than the instrumentation.
Trade Review"...Stewart and Ebel offer a textbook for graduate or advanced undergraduate students in life sciences and a reference for researchers. They introduce the fundamentals of making chemical measurements..." (SciTech Book News, Vol. 24, No. 4, December 2000)
"In general, this is a book, which could be of enormous utility for people working in biochemistry." (The Analyst, 8th October, 2001)
"there was a lot here that I liked and I found myself nodding in agreement" (Chromatographia, November 2001)
"The style is readable and didactic and I think it succeeds as a basic primer." (Chromatographia)
Table of ContentsWater, pH, and Buffers.
Ultraviolet and Visible Absorption Spectrophotometry and Photometry.
Detection Reactions (Colorimetric Reactions).
Enzymes and the Use of Enzymes as Reagents.
Measurement of Enzyme Activities.
Chromatography as a Tool for Measurement of Analytes in Complex Mixtures.
Electrophoresis and Other Electrokinetic Separations.
Enzyme-Linked Immunosorbant Assays.
Assay Quality Control and Data Validation.
Method Selection.
Appendices.
Index.
