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Book Synopsis
Circulating cell-free DNA is poised to transform cancer diagnosis and care; however, it carries technical challenges such as low abundance, fragmentation and sensitivity to biospecimen handling. Development of clinically useful assays hinges on understanding the unique technical aspects of working with cell-free DNA as a substrate. This book provides an in-depth summary of the technical issues impacting cell-free DNA purification from blood plasma and analysis in a cancer context, including design of PCR assays, sequencing library preparation and methylation analysis. Emerging fields such as extracellular vesicles and blood nucleases are also covered, as well as basic biology and considerations pertinent to biobanking.

Cell-free Circulating Dna: Purification And

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A Hardback by Kristina Warton, Goli Samimi

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    View other formats and editions of Cell-free Circulating Dna: Purification And by Kristina Warton

    Publisher: World Scientific Publishing Co Pte Ltd
    Publication Date: 22/08/2022
    ISBN13: 9789811244674, 978-9811244674
    ISBN10: 9811244677
    Also in:
    Biomedical engineering / Medical engineering Medical diagnosis

    Description

    Book Synopsis
    Circulating cell-free DNA is poised to transform cancer diagnosis and care; however, it carries technical challenges such as low abundance, fragmentation and sensitivity to biospecimen handling. Development of clinically useful assays hinges on understanding the unique technical aspects of working with cell-free DNA as a substrate. This book provides an in-depth summary of the technical issues impacting cell-free DNA purification from blood plasma and analysis in a cancer context, including design of PCR assays, sequencing library preparation and methylation analysis. Emerging fields such as extracellular vesicles and blood nucleases are also covered, as well as basic biology and considerations pertinent to biobanking.

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