Description
Book SynopsisThis reference work presents the origins of cells for tissue engineering and regeneration, including primary cells, tissue-specific stem cells, pluripotent stem cells and trans-differentiated or reprogrammed cells. There is particular emphasis on current understanding of tissue regeneration based on embryology and evolution studies, including mechanisms of amphibian regeneration. The book covers the use of autologous versus allogeneic cell sources, as well as various procedures used for cell isolation and cell pre-conditioning , such as cell sorting, biochemical and biophysical pre-conditioning, transfection and aggregation. It also presents cell modulation using growth factors, molecular factors, epigenetic approaches, changes in biophysical environment, cellular co-culture and other elements of the cellular microenvironment. The pathways of cell delivery are discussed with respect to specific clinical situations, including delivery of ex vivo manipulated cells via local and systemic routes, as well as activation and migration of endogenous reservoirs of reparative cells. The volume concludes with an in-depth discussion of the tracking of cells in vivo and their various regenerative activities inside the body, including differentiation, new tissue formation and actions on other cells by direct cell-to-cell communication and by secretion of biomolecules.
Table of ContentsSources.- Primary cells.- Chondrocytes.- Muscle cells.- ß-cells.- Schwann cells.- Cell lines for test systems.- Immortalized primary cells.- STEM.- Bone marrow derived.- Placenta derived cells.- Amnion.- in toto.- Placenta / Chorion.- Cord/Wharton cells.- Amniotic fluid cells.- Adipose derived cells.- Isolation enzymatic.- SVF.- Isolation non-enzymatic.- SVF.- ASC.- Perivascular / Angiogenic cells origin.- Multiple Sources.- Blood.- Olfactory mucosa.- Tendon.- iPS, ES or Transdifferentiation.- ESC.- Many sources and routes to iPS.- Needs for clinical application.- Tools for mechanistic studies.- HaploBank.- Autologous or Allogeneic.- Preconditioning.- Sorting.- Biophysical.- Chemical.- Transfection.- Aggregation.- Sheets.- Microaggregates.- Two level approach.- Modulation.- Growth factors.- miRNA.- Epigenetic approaches.- Biophysical.- Two level approach.- "Student-Teacher".- Coculture.- "Student-School".- Environmental Cues.- Application Routes.- Systemic.- Local.- Endogenous catching.- SDF-1-CXCR4 axis.- Cell Tracking.- Action.- Cell differentiation.- Cell-cell contact.- Secretome.- Learning from embryogenesis / evolution.- Basics of amphibian self regeneration.
