Description
Book SynopsisIsolation and Culture of Osteoblasts.- Isolation and Generation of Osteoclasts.- Isolation, Purification, Generation, and Culture of Osteocytes.- Isolation, Differentiation, and Characterization of Human Bone Marrow Stromal Stem Cells In Vitro and In Vivo.- Isolation and Characterization of Primary Bone Marrow Adipocytes in Rodent Models.- Isolation and Characterization of Circulating Tumor Cells.- 2D and 3D In Vitro Co-Culture for Cancer and Bone Cell Interaction Studies.- Isolation of Extracellular Vesicle Subtypes from Primary Osteoblast Cultures.- Using Cell and Organ Culture Models to Analyze Responses of Bone Cells to Mechanical Stimulation.- Analysis of Signaling Pathways by Western Blotting and Immunoprecipitation.- Analysis of mRNA, miRNA, and DNA in Bone Cells by RT-qPCR and In Situ Hybridization.- Analysis of Transcriptional Regulation in Bone Cells.- The Ex Vivo Organ Culture of Bone and Cartilage.- Ex Vivo Mode