Description
Book SynopsisThis volume provides methods to study bone morphogenetic proteins (BMPs) and BMP modulators in cell culture, in both invertebrate and vertebrate animal models, and for therapeutic applications.
Table of Contents1. Common Cell Lines Used to Study Bone Morphogenetic Proteins (BMPs)
Jessica Ann Cottrell, Donya Burgess, Sarah Michaels, and Melissa B. Rogers
2. In Vitro and In Vivo Osteogenic Differentiation of Human Adipose-Derived Stromal Cells
Clement D Marshall, Elizabeth A Brett, Alessandra L Moore, Derrick C Wan, and Michael T Longaker
3. Cell-based Gene Therapy System for Delivering BMPs
Austin Dickerson, Eleanor L. Davis, Corinne Sonnet, Alan R. Davis, and Elizabeth A. Olmsted-Davis
4. Generation of Endogenous BMP Transcriptional Reporter Cells through CRISPR/Cas9 Genome Editing
Luke D. Hutchinson, Polyxeni Bozatzi, Thomas Macartney, and Gopal P. Sapkota
5. High-Throughput, Biosensor-based Approach to Examine Bone Morphogenetic Protein (BMP) – Receptor Interactions
Senem Aykul and Erik Martinez-Hackert
6. Mutagenesis and Imaging studies of BMP Signaling Mechanisms in C. elegans
Cathy Savage-Dunn, Ryan J. Gleason, Jun Liu, and Richard W. Padgett
7. Gene Regulation of BMP Ligands in Drosophila
Brian G. Stultz and Deborah A. Hursh
8. Using Amphioxus as a Basal Chordate Model to Study BMP Signaling Pathway
Luok Wen Yong, Iryna Kozmikova, and Jr-Kai Yu
9. Proteolytic Activation of Bmps: Analysis of Cleavage in Xenopus Oocytes and Embryos
Hyung-Seok Kim, Autumn McKnite, and Jan L. Christian
10. Imaging and Quantification of P-Smad1/5 in Zebrafish Blastula and Gastrula Embryos
Joseph Zinski, Francesca Tuazon, Yan Huang, Mary Mullins, and David Umulis
11. An Adult Zebrafish Model of Fibrodysplasia Ossificans Progressiva
Melissa LaBonty and Pamela C. Yelick
12. Generation and Identification of Genetically Modified Mice for BMP Receptors
Jingwen Yang and Yuji Mishina
13. Phenotypic Analyses of Genetically Modified Mice for BMP Receptors
Jingwen Yang and Yuji Mishina
14. Immunofluorescent Visualization of BMP Signaling Activation on Paraffin-embedded Tissue Sections
Maaike Alkema, Marie-José Goumans, and Boudewijn P.T. Kruithof
15. Spatial and Quantitative Detection of BMP Activity in Mouse Embryonic Limb Buds
Marcelo Rocha Marques and Jean-Denis Bénazet
16. Pharmacologic Strategies for Assaying BMP Signaling Function
Teresa Dinter, Geoffrey A. Bocobo, and Paul B. Yu
17. Bone Morphogenetic Proteins (BMPs) and Bone Regeneration
Deboleena Kanjilal and Jessica Ann Cottrell
18. Heterotopic Ossification in Mouse Models of Fibrodysplasia Ossificans Progressiva
Salin A. Chakkalakal and Eileen M. Shore
19. Double-Humanized Mouse Model to Study Bone Morphogenetic Protein (BMP) Signaling in Tumor Xenografts
Jenna Newman; Rachel NeMoyer, Andrew Zloza, and John Langenfeld
