Description
Book SynopsisOver the last decade, high--sensitivity calorimetry has developed from a specialist method used mainly by dedicated experts to a major, commercially available tool in the arsenal directed at understanding molecular interactions and stability. Calorimeters have now become commonplace in bioscience laboratories.
Trade Review"...an excellent review of the subject..." (Chromatographia, Vol 62 (7/8) October 2005)
"…recommended to researchers working on thermal analysis in the fields of biochemistry ... pharmacy due to the abundance of practical advice." (Engineering of Life Sciences, 2006)
Table of ContentsPreface.
List of Contributors.
PART I: GENERAL INTRODUCTION.
1. Applications of Biocalorimetry: Binding, Stability and Enzyme Kinetics (R. O’Brien and I. Haq).
PART II: ISOTHERMAL TITRATION CALORIMETRY.
2. Isothermal Titration Calorimetry: A Tutorial (J.A. Thomson and J.E. Ladbury).
3. The Application of Isothermal Titration Calorimetry to Drug Discovery (G. Holdgate. S. Fisher and W. Ward).
4. Dissecting the Thermodynamics of DNA-Protein Interactions (T. Härd).
5. Salt Effects in Ribonuclease-Ligand Interactions: Screening or Competitive Binding? (K.P. Murphy, T.T. Waldron and G.L. Schrift)
6. Thermodynamic-Structure Correlations of Sulfonamide Inhibitor Binding to Carbonic Anhydrase (D. Matulis and M. Todd).
7. Energetics of the Interaction of Human Acidic Fibroblast Growth Factor with Heparin and the Functional Analog Myo-inositol Hexasulfate (M. Guzmán-Casado, M.M. Garcia-Mira, P. Cano-Soldado, J.M. Sanchez-Ruiz and A. Parody-Morreale).
8. Thermodynamics of SH2 Domain Binding (G. Waksman, S. Kumaran and P. Lubman).
9. Titration Calorimetry as a Tool to Determining Thermodyanmic and Kinetic Parameters of Enzymes (M.L. Bianconi).
PART III: DIFFERENTIAL SCANNING CALORIMETRY.
10. Energetics of Site-Specific DNA Recognition by Integrase Tn916 (S. Milev, H.R. Bosshard and I. Jelesarov).
11. Linkage Between Temperature and Chemical Denaturant Effects on Protein Stability: The Intepretation of Calorimetrically-Determined m Values (B. Ibarra-Malero, R. Perez-Jimenez, R. Godoy-Ruiz and J.M. Sanchez-Ruiz).
12. Thermodymic Indications of the Molten Globule State of Cytochrome c Induced by Hydrophobic Salts (A.A. Moosavi-Movahedi and J. Chamani).
13. Microcalorimetry as Applied to Psychrophilic Enzymes (S. D’Amico, D. Georlette, T. Collins, G. Feller and C. Gerday).
14. An Autosampling Differential Scanning Calorimeter for Study of Biomolecular Interactions (V. Plotnikov, A. Rochalski, M. Brandts, J.F. Brandts, S. Williston, V. Frasca and L.-N. Lin).
Index.
