Description
Book SynopsisThis volume covers a wide range of model systems from invertebrate to humans and in vitroandin vivo. Chapters detail methods on squid, aplysia, xenopus, mouse in vivo, ex vivo, rodent primary neurons, human-derived neurons, zebrafish, drosophila in vivo, primary neurons, c. elegans, and in vitro reconstitution assays.Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge,Axonal Transport: Methods and Protocolsaimstofurther our understanding of the trafficking ofcellularcomponents in the nervous system leading to the advancement of basic knowledge that underlines the cell biology of theneuron. The chapters Live imaging of RNA Transport and Translation in Xenopus Retinal Axons, Retrograde Axonal Transport of Neurotrophins in Basal Fore
Table of ContentsPart I: Squid, Aplysia, and Xenopus
1. The Giant Axon of the Squid: A Simple System for Axonal Transport Studies
Joseph DeGiorgis, Marcus Jang, and Elaine L Bearer
2. Live Imaging and Quantitative Analysis of Organelle Transport In Sensory Neurons of Aplysia Californica
Kerriann Badal, Yibo Zhao, Kyle Miller, and Sathyanarayanan Puthanveettil
3. Live imaging of RNA Transport and Translation in Xenopus Retinal Axons
Julie Qiaojin Lin and Jean-Michel Cioni
Part II: Mouse in vivo and ex vivo
4. Imaging Axonal Transport in
Ex Vivo Central and Peripheral Nerves
Stacey Anne Gould, Robert Adalbert, Stefan Milde, and Michael Coleman
5. In vivo Imaging of Axonal Organelle Transport in the Mouse Brain
Johannes Knabbe, Jil Protzmann, and Thomas Kuner
6. Studying Axonal Transport in the Brain by Manganese-Enhanced Magnetic Resonance Imaging (MEMRI)
Elaine L. Bearer, Xiaowei Zhang,and Russell E. Jacobs
Part III: Rodent Primary Neurons
7. Microfluidic Neuromuscular Co-Culture System for Tracking Cell-To-Cell Transfer and Axonal Transport of Labeled Proteins
Ariel Ionescu and Eran Perlson
8. Imaging Diversity in Slow Axonal Transport
Archan Ganguly and Subhojit Roy
9. Methods and Applications of Campenot Trichamber Neuronal Cultures for the Study of Neuroinvasive Viruses
Wesley M. Tierney, Ian A. Vicino, Stella Y. Sun, Wah Chiu, Esteban A. Engel, Matthew P. Taylor, and Ian B. Hogue
10. Molecular Analysis of Axonal Transport Dynamics Upon Modulation of Microtubule Acetylation
Silvia Turchetto, Romain Le Bail, Loic Broix, and Laurent Nguyen
11. Live-cell Imaging of RNA Transport In Axons Of Cultured Primary Neurons
Tabitha J. Hees and Angelika B. Harbauer
12. Visualizing Vesicle-Bound Kinesins in Cultured Hippocampal Neurons
Andrew Montgomery, Alex Garbouchian, and Marvin Bentley
13. Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons.
Arman Shekari and Margaret Fahnestock
14. Use of Microfluidics Chambers to Image Axonal Transport In Adult Sensory Neurons
Maria Fransiska Emily, Lokesh Agrawal, Paolo Barzaghi, Miki Otsuki, and Marco Terenzio
Part IV: Human-derived Neurons
15. High-resolution Imaging of Mitochondria and Mitochondrial Nucleoids in Differentiated SH-SY5Y Cells
Emily Annuario, Kristal Y-W Ng, and Alessio Vagnoni
16. Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neurons
Arpan R. Mehta, Siddharthan Chandran, and Bhuvaneish T. Selvaraj
Part V: Zebrafish
17. In vivo Live Imaging of Axonal Transport In Developing Zebrafish Axons
Melody Atkins, Jamilé Hazan, and Coralie Fassier
18. Visualizing the Intracellular Trafficking in Zebrafish Mauthner Cells
Rongchen Huang, Yang Xu, Min Chen, Leiqing Yang, Xinliang Wang, Yueru Shen, Yubin Huang, Bing Hu
Part VI:
Drosophila In Vivo, Ex Vivo, and Primary Neurons
19. Dissection and Direct Imaging of Axonal Transport in Drosophila Segmental Nerves
William M. Saxton, Angeline Lim, and Inna Djagaeva
20. Detailed Imaging of Mitochondrial Transport and Precise Manipulation of Mitochondrial Function with Genetically-Encoded Photosensitizers in Adult Drosophila Neurons
Francesca Mattedi, George Chennell, and Alessio Vagnoni
21. Mitochondrial DNA Transport in Drosophila neurons
Joseph M. Bateman
22. Live imaging of Axonal Transport in the Adult Drosophila Central Nervous System
Wayne Robinson and Tanja A Godenschwege
23. Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport
André Voelzmann and Natalia Sánchez-Soriano
24. High-resolution Live-Imaging of Axonal RNP granules in
Drosophila Pupal Brain Explants
Caroline Medioni, Jeshlee Vijayakumar, Anne Ephrussi, and Florence Besse
Part VII:
C. elegans
25. Analyzing the Impact of Gene Mutations on Axonal Transport in Caenorhabditis elegans
Yuzu Anazawa and Shinsuke Niwa
26. Methods to Quantify and Relate Axonal Transport Defects to Changes In C. Elegans Behavior
Syed Nooruzuha Barmaver, Muniesh Muthaiyan Shanmugam, and Oliver Ingvar Wagner
27. Imaging Intracellular Trafficking in Neurons of C. elegans.
Sravanthi S P Nadiminti and Sandhya P Koushika
Part VIII: In vitro Reconstitution Assays
28. In vitro Reconstitution of Molecular Motor-Driven Mitochondrial Transport
Verena Henrichs, Jakub Rohlena, Marcus Braun, and Zdenek Lansky
29. In vitro Reconstitution of Kinesin-Based, Axonal mRNA Transport
Julia Grawenhoff, Sebastian Baumann, and Sebastian P. Maurer
