Description
Book SynopsisThis volume details generation of gene-edited cell lines and organisms as models for human diseases, pest control, and large animal welfare and production outcomes. Chapters guide readers through gene regulation, editing, screening of cell lines, genome editing, and an overview of the tools for efficient genome editing including; ZFNs, TALENs, and CRISPR. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step- by-step, readily reproducible protocols.
Authoritative and cutting-edge, Applications of Genome Modulation and Editing aims to be a useful and practical guide for researchers to commence or advance their study in this field.
Table of ContentsPart I: Gene Editing Approaches
1. Historical DNA manipulation Overview
LLuis Montoliu
2. Tools for efficient genome editing; ZFN, TALEN, and CRISPR
Yasaman Shamshirgaran,,Jun Liu, Huseyin Sumer, Paul J. Verma and Amir Taheri-Ghahfarokhi
Part II: Genetic Manipulation Methods
3. Efficient generation of stable cell lines with inducible neuronal transgene expression using the piggyBac transposon system
Jinchao Gu, Huseyin Sumer and Brett Cromer
4. Modifying Bacterial Artificial Chromosomes for Extended Genome Modification Hannah Auch, Nikolai Klymiuk and Petra Runa-Vochozkova
5. Site specific editing of the genome in Pluripotent cells Luis Ortega and Joseph Rosenbluh
6. Targeting the AAVS1 site with transgene cassette by CRISPR/Cas9 with an inducible transgene cassette for the to neuronal differentiation of human pluripotent stem cells
Jinchao Gu, Ben Rollo, Huseyin Sumer and Brett Cromer
7. Microinjection of zygotes for CRISPR/Cas9 mediated insertion of transgenes into the murine Rosa26 safe harbour
Fabien Delerue and Lars M. Ittner
8. CRISPR-on for endogenous activation of SMARCA4 expression in bovine embryos
Virgilia Alberio, Virginia Savy and Daniel F. Salamone
Part III: Applications of Genome Manipulation
9. CRISPR/Cas mutagenesis to generate novel traits in Bactrocera tryoni for Sterile Insect Technique
Amanda Choo, Elisabeth Fung, Thu N.M. Nguyen, Anzu Okada and Peter Crisp
10. CRISPR/Cas9 Genome Editing in the New World Screwworm and Australian Sheep Blowfly
Daniel F. Paulo, Megan E. Williamson and Maxwell J. Scott
11. Generation of gene drive mice for invasive pest population suppression
Mark Bunting, Chandran Pfitzner, Luke Gierus, Melissa White, Sandra Piltz and Paul Thomas
Part IV: Large animal models of human disease
12. Generation of a human deafness sheep model using the CRISPR/Cas system
Martina Crispo, Vanessa Chenouard, Pedro dos Santos-Neto, Laurent Tesson, Marcela Souza, JeanMarie Heslan, Federico Cuadro, Ignacio Anegón and Alejo Menchaca
13. Targeted Gene Editing in Porcine Germ Cells
Taylor Goldsmith, Alla Bondareva, Dennis Webster, Anna Laura Voigt, Lin Su, Daniel F. Carlson and Ina Dobrinski
14. Generating a Heat Tolerance Mouse Model
Jun Liu and Paul J. Verma
Part V: Large Animal Welfare and Production Outcomes
15. Generation of pigs that produce single sex progeny
Björn Petersen and Stefanie Kurtz
16. Generation of Double-muscled Sheep and Goats by CRISPR/Cas9-mediated Knockout of the Myostatin Gene
Peter Kalds, Martina Crispo, Chao Li, Laurent Tesson, Ignacio Anegon, Yulin Chen, Xiaolong Wang and Alejo Menchaca
Part VI: Concluding Remarks
17. Regulatory and policy considerations around applications of genome editing in agriculture
Steffi Friedrichs, Karinne Ludlowand Peter Kearns
