Description
Book SynopsisCell Culture Techniques.- Solutions Preparation.- Standard Operating Procedure (SOP) for Maintaining Sterility in the Laminar Flow Hood During Cell Culture Procedures.- Protocol for Maintenance, Isolation, and Primary Culture of Ehrlich Tumor Cells.- Stem Cell Cultivation and Maintenance.- Protocol for Isolation of Stem Cells from Rabbit Adipose Tissue.- Protocol for Isolation of Lung Fibroblasts from Mouse Tissue.- Protocol for Isolation of Mice Fat Stem Cells.- Protocol for Isolation of Mouse Adipose-Derived Stem Cells (ADSCs).- Protocol for Isolation of Bone Marrow Stem Cells.- Extraction of Mesenchymal Stem Cells (MSCs) from Human Liposuction Fat.- Human Bone Marrow Extraction Protocol.- Mesenchymal Stem Cells (MSCs) Differentiation Protocols.- cDNA Synthesis and Quantitative PCR (qPCR) for Gene Expression Analysis.- Preparation and Optimization of Reagents for Genetic Analysis and Infectious Disease Diagnosis.- DNA and RNA Extraction from Plasma Using Magnetized Silica.- RNA Extraction Using Trizol (6-Well Plate Method).- Agarose Gel Electrophoresis for RNA Integrity Assessment.- DNA Purification Using the Wizard Plus SV Minipreps DNA Purification System (Promega).- Detection of Reactive Oxygen Species (ROS) in Embryos.- Determination of Protein Concentration: Sedmak and Grossberg Method.- Cell Sorting of Fluorescent Zebrafish Cells.- Cell Disaggregation for Flow Cytometry Analysis of Fluorescent Zebrafish Cells.- RNA Extraction from Zebrafish Embryos for RT-qPCR.- Extraction of Genomic DNA.- RNA Extraction from HEK293.- Agarose Gel Preparation for RNA and DNA Electrophoresis.- Zebrafish Genotyping by Fin Clipping.- In Situ Hybridization of Zebrafish Embryos.- Concepts and Practices in Flow Cytometry.- In Situ Apoptosis Assay.- Flow Cytometry Protocols for Assessing Membrane Integrity, Cell Cycle, and Apoptosis in Treated Cells.- Leukocyte Reading Protocol on the Flow Cytometer.- Flow Cytometry Protocol for Mouse Sperm Cells (BD Accuri C6+).- Extraction of Yolk Proteins from 24 HPF Zebrafish Embryos.- PFA Fixation for Zebrafish Embryos.- Thioflavin T (ThT) Fluorescence Measurement.- Alcian Blue Staining Protocol.- Zebrafish Embryo Staining with Nicotinamide Adenine (NA).- Western Blot on Zebrafish Embryos.- In Vitro Micronucleus Assay in Binucleated CHO-K1 Cells.- In Vitro Assessment of Genotoxicity Using the Comet Assay in CHO-K1 Cells.- Treatment and Cell Collection for Chromosomal Aberration Test.- Electrophoretic Mobility Shift Assay (EMSA): Non-Radioactive, Band Shift, Gel Shift, or Gel Retardation.- MTT Assay Protocol.- Spectropolarimeter (Jasco-1500) Operation Protocol.- Immunoprecipitation Protocol (Tuning).- qPCR EvaGreen Hot FIREPol Mix.- Synthesis of RNA-Digoxigenin Probe.- Thermal Difference Spectra (TDS).- Generation of Competent Bacteria.- Retro Transcription via STEM LOOP.- In Vitro Transcription with the mMessage mMachine SP6 Transcription Kit.- Transfection in HEK293 Cells.- HeLa Transfections with Lipofectamine 3000.- Chemical Competent Cell Transformation.
