Description

Includes all of the information required to produce monoclonal antibodies in the laboratory and to prepare them for use in a multitude of given applications. Production procedures are treated in chronological order, beginning with basic tissue culture techniques, immunization strategies and screening test design, followed by production of hybridoma cell lines and basic antibody characterization, purification and labeling. Each chapter contains explanatory text on each step with comparative analysis of methods where appropriate. All necessary experimental protocols are presented in a self-contained format that is easy to follow in the laboratory. Alternative protocols are provided where relevant; for others not included in full, source references are presented. Surveys the current status of human hybridoma production and antibody engineering using molecular biology techniques.

A Practical Guide to Monoclonal Antibodies

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£480.95

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Paperback / softback by J. Eryl Liddell , A. Cryer

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Short Description:

Includes all of the information required to produce monoclonal antibodies in the laboratory and to prepare them for use in... Read more

    Publisher: John Wiley & Sons Inc
    Publication Date: 29/04/1991
    ISBN13: 9780471929055, 978-0471929055
    ISBN10: 0471929050

    Number of Pages: 206

    Non Fiction , Mathematics & Science , Education

    Description

    Includes all of the information required to produce monoclonal antibodies in the laboratory and to prepare them for use in a multitude of given applications. Production procedures are treated in chronological order, beginning with basic tissue culture techniques, immunization strategies and screening test design, followed by production of hybridoma cell lines and basic antibody characterization, purification and labeling. Each chapter contains explanatory text on each step with comparative analysis of methods where appropriate. All necessary experimental protocols are presented in a self-contained format that is easy to follow in the laboratory. Alternative protocols are provided where relevant; for others not included in full, source references are presented. Surveys the current status of human hybridoma production and antibody engineering using molecular biology techniques.

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