{"product_id":"west-nile-virus-9781071627594","title":"West Nile Virus","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eThis volume provides researchers with the most updated understanding of the basics of West Nile Virus (WNV). Chapters focus on the biology, virology, epidemiology, pathogenesis, as well as the step-by-step molecular, cellular, and statistical methods to study WNV infection in cell culture, mosquitos, animal models, and human clinical specimen.  Written in the highly successful \u003ci\u003eMethods in Molecular Biology \u003c\/i\u003eseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e \u003cbr\u003e Cutting-edge and comprehensive, \u003ci\u003eWest Nile Virus: Methods and Protocols \u003c\/i\u003eaims to be a valuable resource for all researchers interested in learning more about this important and developing field.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e1. Introduction to West Nile Virus\u003c\/p\u003e  \u003cp\u003eShazeed-Ul Karim and Fengwei Bai\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e2. Quantification of West Nile virus by Plaque-Forming Assay\u003c\/p\u003e  \u003cp\u003eBiswas Neupane and Fengwei Bai\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  3. Viral titer quantification of West Nile virus by immunostaining plaque assay \u003cp\u003e\u003c\/p\u003e  \u003cp\u003eNa Li, Cheng-Lin Deng, Bo Zhang and Han-Qing Ye\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e4. Isolate and Culture Mouse Primary Neurons for West Nile Virus Infection\u003c\/p\u003e  \u003cp\u003eFarzana Nazneen and Fengwei Bai\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e5. Isolation of Murine Bone Marrow Derived Neutrophils for Infection Modeling\u003c\/p\u003e  \u003cp\u003eLaurel Duty\u003csup\u003e \u003c\/sup\u003eand Amber M. Paul\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e6. Methods to study West Nile Virus infection, and the virus-induced inflammation in the brain in a murine model\u003c\/p\u003e   \u003cp\u003eHuanle Luo and Tian Wang\u003c\/p\u003e  \u003cp\u003e\u003csup\u003e \u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e7. Detection of West Nile virus envelope protein in brain tissue with an immunohistochemical assay\u003c\/p\u003e  \u003cp\u003eKathleen T. Yee and Douglas E. Vetter\u003c\/p\u003e   \u003cp\u003e\u003c\/p\u003e  \u003cp\u003e8. Quantitative Analysis of B Cell Subpopulations in Bone Marrow by Flow Cytometry\u003c\/p\u003e  \u003cp\u003eTingting Geng, Penghua Wang\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e9. Isolation of exosomes or extracellular vesicles from West Nile Virus-infected \u003c\/p\u003e  N2a cells, Primary Cortical Neurons and Brain Tissues\u003cp\u003e\u003c\/p\u003e  \u003cp\u003eHameeda Sultana\u003csup\u003e \u003c\/sup\u003eand Girish Neelakanta \u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e10. Protocol to Study West Nile Virus Infection in Brain Slices In Vitro\u003c\/p\u003e  \u003cp\u003eParminder J.S. Vig\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e11. Studying Virus-Host Interactions with CRISPR technology\u003c\/p\u003e  \u003cp\u003eRong Zhang\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  12. Protocol of detection of West Nile Virus in Clinical Samples\u003cp\u003e\u003c\/p\u003e  \u003cp\u003eHephzibah Nwanosike, Freedom M. Green, Kristy O. Murray, Jill E. Weatherhead, and Shannon E. Ronca\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e13. Detection and Analysis of West Nile Virus Structural Protein Genes in Animal Or Bird Samples\u003c\/p\u003e  \u003cp\u003eGili Schvartz, Sharon Karniely, Roberto Azar, Areej Kabat, Amir Steinman, and Oran Erster\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e14. Mosquito surveillance for West Nile Virus\u003c\/p\u003e  \u003cp\u003eDonald A. Yee, Ary Faraji, and Ilia Rochlin\u003c\/p\u003e  \u003cp\u003e15. Molecular surveillance of West Nile virus in mosquitoes and sentinel chickens\u003c\/p\u003e  \u003cp\u003eSteven T Peper\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e16. Statistical tools for West Nile Virus Disease Analysis \u003c\/p\u003e  \u003cp\u003eMathew J Ward, Meytar Sorek-Hamer, Krishna Vemur, and Nicholas B DeFelice\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e17. Analytical Approaches to Uncover Genetic Associations for Rare Outcomes: Lessons from West Nile Neuroinvasive Disease\u003c\/p\u003e  \u003cp\u003eMegan E. Cahill and Ruth R. Montgomery\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e18. Safety procedures to work with West Nile virus in biosafety level 3 facilities \u003c\/p\u003e  \u003cp\u003eFreedom M. Green and Shannon E. Ronca\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e19. Development of antibody-based therapeutics against West Nile virus in plants\u003c\/p\u003e  Haiyan Sun, Joshua Lesio, and Qiang Chen\u003cp\u003e\u003c\/p\u003e","brand":"Springer-Verlag New York Inc.","offers":[{"title":"Default Title","offer_id":51039240421719,"sku":"9781071627594","price":161.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781071627594.jpg?v=1750943024","url":"https:\/\/bookcurl.com\/products\/west-nile-virus-9781071627594","provider":"Book Curl","version":"1.0","type":"link"}