{"product_id":"viroids-9781071614631","title":"Viroids","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eThis volume explores the latest methods used by researchers to study the detection, characterization, and various aspects of viroids. The chapters in this book are organized into seven parts and cover topics such as detection methods based on the biology of viroids; detection techniques based on electrophoresis and hybridization techniques; PCR-based techniques that provide high degrees of sensitivity; emerging area of nucleic acid sequence-based technology; and emerging techniques in viroid research such as RNA silencing, splicing, and viroid structure. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \u003cbr\u003e\u003cbr\u003eCutting-edge and comprehensive, \u003ci\u003eViroids: Methods and Protocols\u003c\/i\u003e is a valuable resource for researchers and gradua\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003ePreface…\u003cbr\u003eTable of Contents…\u003cbr\u003eContributing Authors…\u003cbr\u003e\u003cbr\u003ePart I Biological Techniques\u003cbr\u003e\u003cbr\u003e1.\tStudy and Detection of Citrus Viroids in Woody Hosts\u003cbr\u003eRobert R. Krueger and Georgios Vidalakis\u003cbr\u003e\u003cbr\u003e2.\tDetection and Characterization of Viroids via Biological Assays on Herbaceous Hosts\u003cbr\u003eRosemarie W. Hammond\u003cbr\u003e\u003cbr\u003e3.\tAnalysis on RNA Motif-Based RNA Trafficking in Plants\u003cbr\u003eHeather N. Smith, Junfei Ma, and Ying Wang\u003cbr\u003e\u003cbr\u003e4.\tIsolation and Transfection of Citrus Protoplasts with Citrus Exocortis Viroid\u003cbr\u003eSubhas Hajeri, James Ng, Jude Grosser, and Georgios Vidalakis\u003cbr\u003e\u003cbr\u003ePart II Electrophoresis and Hybridization Techniques\u003cbr\u003e\u003cbr\u003e5.\tHigh-Throughput RNA Extraction from Citrus Tissues for the Detection of Viroids\u003cbr\u003eTyler Dang, Fatima Osman, Jinbo Wang, Tavia Rucker, Sohrab Bodaghi, Shih-hua Tan, Deborah Pagliaccia, Irene Lavagi-Craddock, and Georgios Vidalakis\u003cbr\u003e\u003cbr\u003e6.\tExtraction and Purification of Viroids from Herbaceous Hosts\u003cbr\u003eRosemarie W. Hammond\u003cbr\u003e\u003cbr\u003e7.\tDetection of Viroids by sPAGE Gel Electrophoresis\u003cbr\u003eNúria Duran-Vila\u003cbr\u003e\u003cbr\u003e8.\tTwo-Dimensional Polyacrylamide-Gel Electrophoresis Analysis of Viroid RNAs\u003cbr\u003eJosé-Antonio Daròs\u003cbr\u003e\u003cbr\u003e9.\tA Simplified Dot-Blot Hybridization Protocol for Potato spindle tuber viroid Detection in Solanaceae\u003cbr\u003eOxana Kekstidou, Christina Varveri, and Nikon Vassilakos\u003cbr\u003e\u003cbr\u003e10.\tGel Blot Hybridization for Viroids\u003cbr\u003ePedro Serra\u003cbr\u003e\u003cbr\u003e11.\tTissue Print Hybridization for Detection and Characterization of Viroids\u003cbr\u003eMaria S. Kaponi, Teruo Sano, and Panayota E. Kyriakopoulou\u003cbr\u003e\u003cbr\u003e12.\tFluorescein-Based Electrophoretic Mobility Shift Assay\u003cbr\u003eShachinthaka D. Dissanayaka Mudiyanselage and Ying Wang\u003cbr\u003e\u003cbr\u003ePart III PCR Techniques\u003cbr\u003e\u003cbr\u003e13.\t Detection of Viroids by RT-PCR\u003cbr\u003eNerida J. Donovan, Grant A. Chambers, and Mengji Cao \u003cbr\u003e\u003cbr\u003e14.\t Detection of Viroids using RT-qPCR\u003cbr\u003eSubhas Hajeri, Georgios Vidalakis, and Raymond Yokomi\u003cbr\u003e\u003cbr\u003e15.\t Multiplex RT-PCR \u003cbr\u003eFrancesco Faggioli and Marta Luigi\u003cbr\u003e\u003cbr\u003e16.\t Real-Time Detection of Viroids using Singleplex and Multiplex Quantitative Polymerase Chain Reaction\u003cbr\u003eFatima Osman and Georgios Vidalakis\u003cbr\u003e\u003cbr\u003e17.\t Reverse Transcription-Loop Mediated Isothermal AMPlification (RT-LAMP) at the Service of Viroid Detection\u003cbr\u003eIraklis N. Boubourakas and Panayota E. Kyriakopoulou\u003cbr\u003e\u003cbr\u003e18.\t SYBR ® Green RT-qPCR for the Universal Detection of Citrus Viroids\u003cbr\u003eGeorgios Vidalakis, Jinbo Wang, Tyler Dang, Tavia Rucker, Sohrab Bodaghi, Shi-hua Tan, Irene Lavagi-Craddock, Alexandra Syed, Gerardo Uribe, Yi Hsieh, and Joana Carbajal-Moreno\u003cbr\u003e\u003cbr\u003e19.\tDetection of Avocado sunblotch and Other Viroids using RNA Filter Paper Capture and RT-PCR\u003cbr\u003eDeborah M. Mathews, Sohrab Bodaghi, James A. Heick, and J. Allan Dodds\u003cbr\u003e\u003cbr\u003ePart IV Cloning and Sequencing Techniques\u003cbr\u003e\u003cbr\u003e20.\t Cloning and Sequencing of Viroids\u003cbr\u003eRosemarie W. Hammond\u003cbr\u003e\u003cbr\u003e21.\t QuantiGene Plex Assay: A Method for High-Throughput Multiplex Citrus Viroid Detection and Identification\u003cbr\u003eTyler Dang, Jinbo Wang, Tavia Rucker, Sohrab Bodaghi, Irene Lavagi-Craddock, and Georgios Vidalakis\u003cbr\u003e\u003cbr\u003e22.\t Culture-Independent Discovery of Viroids by Deep Sequencing and Computational Algorithms\u003cbr\u003eAli Raza, Shou-Wei Ding, and Qingfa Wu \u003cbr\u003e\u003cbr\u003e23.\t An In Silico Detection of a Citrus Viroid from Raw High Throughput Sequencing Data\u003cbr\u003eTyler Dang, Andres Espindola, Georgios Vidalakis, and Kitty Cardwell\u003cbr\u003e\u003cbr\u003ePart V RNA Silencing Techniques\u003cbr\u003e\u003cbr\u003e24.\t Detection of Viroid RNA and vd-siRNA in N. benthamiana Plants: Northern Blot Analyses for Viroid and vd-siRNAs\u003cbr\u003eKonstantina Katsarou, Nikoleta Kryovrysanaki, and Kriton Kalantidis\u003cbr\u003e\u003cbr\u003ePart VI Splicing Techniques\u003cbr\u003e\u003cbr\u003e25.\t Production of Recombinant RNA in Escherichia coli Using Eggplant Latent Viroid as a Scaffold\u003cbr\u003eBeltrán Ortolá and José-Antonio Daròs\u003cbr\u003e\u003cbr\u003ePart VII Structural Analysis\u003cbr\u003e\u003cbr\u003e26.\t Predicting the Structure of a Viroid\u003cbr\u003eGerhard Steger\u003cbr\u003e\u003cbr\u003eSubject Index List…\u003c\/p\u003e","brand":"Springer-Verlag New York Inc.","offers":[{"title":"Default Title","offer_id":49406770872663,"sku":"9781071614631","price":179.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781071614631.jpg?v=1730497052","url":"https:\/\/bookcurl.com\/products\/viroids-9781071614631","provider":"Book Curl","version":"1.0","type":"link"}