{"product_id":"timeresolved-mass-spectrometry-9781118887325","title":"TimeResolved Mass Spectrometry","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eTime is an important factor in physical and natural sciences. It characterizes the progress of chemical and biochemical processes. Mass spectrometry provides the means to study molecular structures by detecting gas-phase ions with the unique mass-to-charge ratios. Time-resolved mass spectrometry (TRMS) allows one to differentiate between chemical states that can be observed sequentially at different time points. Real-time mass spectrometric monitoring enables recording data continuously with a specified temporal resolution. The TRMS approaches  introduced during the past few decades  have shown temporal resolutions ranging from hours down to microseconds and beyond.\u003cbr\u003eThistext covers the key aspects of TRMS. It introduces ion sources, mass analyzers, and interfaces utilized in time-resolved measurements; discusses the influence of data acquisition and treatment; finally, it reviews most prominent applications of TRMS  in the studies of reaction kinetics and mechanism, physicochem\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003eAuthor Biographies xi\u003c\/p\u003e \u003cp\u003ePreface xiii\u003c\/p\u003e \u003cp\u003eAcknowledgments xv\u003c\/p\u003e \u003cp\u003eList of Acronyms xvii\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1. Introduction 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 Time in Chemistry 1\u003c\/p\u003e \u003cp\u003e1.2 Mass Spectrometry 3\u003c\/p\u003e \u003cp\u003e1.3 Time-resolved Mass Spectrometry 5\u003c\/p\u003e \u003cp\u003e1.4 Dynamic Matrices 6\u003c\/p\u003e \u003cp\u003e1.5 Real-time vs. Single-point Measurements 6\u003c\/p\u003e \u003cp\u003e1.6 Further Reading 7\u003c\/p\u003e \u003cp\u003eReferences 7\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2. Ion Sources for Time-resolved Mass Spectrometry 11\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Electron Ionization 12\u003c\/p\u003e \u003cp\u003e2.2 Chemical Ionization 14\u003c\/p\u003e \u003cp\u003e2.3 Atmospheric Pressure Chemical Ionization 18\u003c\/p\u003e \u003cp\u003e2.4 Electrospray Ionization 19\u003c\/p\u003e \u003cp\u003e2.5 Atmospheric Pressure Photoionization 24\u003c\/p\u003e \u003cp\u003e2.6 Desorption\/Ionization 25\u003c\/p\u003e \u003cp\u003e2.6.1 Fast Atom Bombardment 26\u003c\/p\u003e \u003cp\u003e2.6.2 Laser Desorption\/Ionization 27\u003c\/p\u003e \u003cp\u003e2.7 Innovations in the 21st Century 33\u003c\/p\u003e \u003cp\u003e2.7.1 Ion Sources Derived from Electrospray Ionization 34\u003c\/p\u003e \u003cp\u003e2.7.2 New Ion Sources Derived from Laser Desorption\/Ionization 39\u003c\/p\u003e \u003cp\u003e2.7.3 Plasma-based Ion Sources 40\u003c\/p\u003e \u003cp\u003e2.8 Concluding Remarks 43\u003c\/p\u003e \u003cp\u003eReferences 43\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3. Mass Analyzers for Time-resolved Mass Spectrometry 53\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Overview 53\u003c\/p\u003e \u003cp\u003e3.2 Individual Mass Analyzers 54\u003c\/p\u003e \u003cp\u003e3.2.1 Time-of-flight Mass Analyzers 54\u003c\/p\u003e \u003cp\u003e3.2.2 Quadrupole Mass Analyzers 57\u003c\/p\u003e \u003cp\u003e3.2.3 Sector Mass Analyzers 67\u003c\/p\u003e \u003cp\u003e3.2.4 Fourier-transform Mass Analyzers 70\u003c\/p\u003e \u003cp\u003e3.3 Integrated Analytical Techniques 77\u003c\/p\u003e \u003cp\u003e3.3.1 Hybrid Mass Spectrometers 77\u003c\/p\u003e \u003cp\u003e3.3.2 Ion Activation Methods 82\u003c\/p\u003e \u003cp\u003eReferences 85\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4. Interfaces for Time-resolved Mass Spectrometry 89\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Molecules in Motion 89\u003c\/p\u003e \u003cp\u003e4.2 Time-resolved Mass Spectrometry Systems 104\u003c\/p\u003e \u003cp\u003e4.2.1 Photochemical Processes 104\u003c\/p\u003e \u003cp\u003e4.2.2 Off-line Interfaces 107\u003c\/p\u003e \u003cp\u003e4.2.3 Membrane Interfaces 107\u003c\/p\u003e \u003cp\u003e4.2.4 Electrospray Ionization 108\u003c\/p\u003e \u003cp\u003e4.2.5 Desorption Electrospray Ionization 115\u003c\/p\u003e \u003cp\u003e4.2.6 Other Interfaces Derived from Electrospray Ionization 116\u003c\/p\u003e \u003cp\u003e4.2.7 Interfaces for High-throughput Screening 118\u003c\/p\u003e \u003cp\u003e4.2.8 Interfaces Using Laser Light 118\u003c\/p\u003e \u003cp\u003e4.2.9 Interfaces Using Plasma State 119\u003c\/p\u003e \u003cp\u003e4.2.10 Electrochemical Mass Spectrometry 120\u003c\/p\u003e \u003cp\u003e4.2.11 Aerosol Mass Spectrometry 121\u003c\/p\u003e \u003cp\u003e4.2.12 Proton-transfer Reaction Mass Spectrometry 124\u003c\/p\u003e \u003cp\u003e4.2.13 Examples of Other Interfaces 124\u003c\/p\u003e \u003cp\u003e4.3 Concluding Remarks 126\u003c\/p\u003e \u003cp\u003eReferences 127\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5. Balancing Acquisition Speed and Analytical Performance of Mass Spectrometry 157\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Overview 157\u003c\/p\u003e \u003cp\u003e5.2 Spectrum Acquisition Speed 157\u003c\/p\u003e \u003cp\u003e5.2.1 Spectrum Acquisition Time 158\u003c\/p\u003e \u003cp\u003e5.2.2 Duty Cycle 159\u003c\/p\u003e \u003cp\u003e5.3 Relationship between Spectrum Acquisition Time and Mass Spectrometer Performance 161\u003c\/p\u003e \u003cp\u003e5.3.1 Mass Resolving Power 161\u003c\/p\u003e \u003cp\u003e5.3.2 Mass Accuracy 163\u003c\/p\u003e \u003cp\u003e5.3.3 Sensitivity and Detection Limit 165\u003c\/p\u003e \u003cp\u003eReferences 167\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6. Hyphenated Mass Spectrometric Techniques 169\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction 169\u003c\/p\u003e \u003cp\u003e6.1.1 Chromatography 169\u003c\/p\u003e \u003cp\u003e6.1.2 Electrophoresis 172\u003c\/p\u003e \u003cp\u003e6.2 Separation Techniques Coupled with Mass Spectrometry 174\u003c\/p\u003e \u003cp\u003e6.3 Ion-mobility Spectrometry 183\u003c\/p\u003e \u003cp\u003e6.4 Other Hyphenated Systems 185\u003c\/p\u003e \u003cp\u003e6.5 Influence of Data Acquisition Speed 187\u003c\/p\u003e \u003cp\u003e6.6 Concluding Remarks 187\u003c\/p\u003e \u003cp\u003eReferences 189\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7. Microfluidics for Time-resolved Mass Spectrometry 195\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Overview 195\u003c\/p\u003e \u003cp\u003e7.2 Fabrication 195\u003c\/p\u003e \u003cp\u003e7.3 Microreaction Systems 197\u003c\/p\u003e \u003cp\u003e7.4 Hydrodynamic Flow 198\u003c\/p\u003e \u003cp\u003e7.5 Coupling Microfluidics with Mass Spectrometry 200\u003c\/p\u003e \u003cp\u003e7.6 Examples of Applications 204\u003c\/p\u003e \u003cp\u003e7.7 Digital Microfluidics 209\u003c\/p\u003e \u003cp\u003e7.8 Concluding Remarks 211\u003c\/p\u003e \u003cp\u003eReferences 212\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8. Quantitative Measurements by Mass Spectrometry 217\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 The Challenge of Quantitative Mass Spectrometry Measurements 217\u003c\/p\u003e \u003cp\u003e8.1.1 (I) Instrument 218\u003c\/p\u003e \u003cp\u003e8.1.2 (II) Sample 219\u003c\/p\u003e \u003cp\u003e8.2 Selection of Instrument 221\u003c\/p\u003e \u003cp\u003e8.3 Solutions to Quantitative Mass Spectrometry 221\u003c\/p\u003e \u003cp\u003e8.3.1 Quantification with Separation 221\u003c\/p\u003e \u003cp\u003e8.3.2 Quantification without Separation 226\u003c\/p\u003e \u003cp\u003e8.4 Data Treatment 227\u003c\/p\u003e \u003cp\u003e8.5 Concluding Remarks 228\u003c\/p\u003e \u003cp\u003eReferences 228\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9. Data Treatment in Time-resolved Mass Spectrometry 231\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 Overview 231\u003c\/p\u003e \u003cp\u003e9.2 Definition of Terms 232\u003c\/p\u003e \u003cp\u003e9.3 Spectral Patterns 232\u003c\/p\u003e \u003cp\u003e9.3.1 Accurate Mass 233\u003c\/p\u003e \u003cp\u003e9.3.2 Mass Calibration 235\u003c\/p\u003e \u003cp\u003e9.3.3 Singly Charged Molecules 235\u003c\/p\u003e \u003cp\u003e9.3.4 Multiply Charged Molecules 238\u003c\/p\u003e \u003cp\u003e9.4 Mass Accuracy 238\u003c\/p\u003e \u003cp\u003e9.5 Structural Derivation 240\u003c\/p\u003e \u003cp\u003e9.5.1 Unsaturation and Ring Moieties 241\u003c\/p\u003e \u003cp\u003e9.5.2 Nitrogen Rule 241\u003c\/p\u003e \u003cp\u003e9.5.3 Functional Groups 241\u003c\/p\u003e \u003cp\u003e9.6 Molecule Abundance 242\u003c\/p\u003e \u003cp\u003e9.6.1 Signal Intensity 242\u003c\/p\u003e \u003cp\u003e9.6.2 Quantity Calibration 243\u003c\/p\u003e \u003cp\u003e9.6.3 Dynamic Range 244\u003c\/p\u003e \u003cp\u003e9.7 Time-dependent Data Treatment 245\u003c\/p\u003e \u003cp\u003eReferences 246\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10. Applications in Fundamental Studies of Physical Chemistry 249\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Overview 249\u003c\/p\u003e \u003cp\u003e10.2 Chemical Kinetics 250\u003c\/p\u003e \u003cp\u003e10.2.1 Quantum Chemistry 250\u003c\/p\u003e \u003cp\u003e10.2.2 Reaction Kinetics 253\u003c\/p\u003e \u003cp\u003e10.3 Chemical Equilibrium 259\u003c\/p\u003e \u003cp\u003eReferences 263\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11. Application of Time-resolved Mass Spectrometry in the Monitoring of Chemical Reactions 269\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e11.1 Organic Reactions 270\u003c\/p\u003e \u003cp\u003e11.2 Catalytic Reactions 279\u003c\/p\u003e \u003cp\u003e11.3 Photochemical Reactions 282\u003c\/p\u003e \u003cp\u003e11.4 Concluding Remarks 284\u003c\/p\u003e \u003cp\u003eReferences 284\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12. Applications of Time-resolved Mass Spectrometry in the Studies of Protein Structure Dynamics 291\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e12.1 Electrospray Ionization in Protein Studies 292\u003c\/p\u003e \u003cp\u003e12.2 Mass Spectrometry Strategies for Ultra-fast Mixing and Incubation 295\u003c\/p\u003e \u003cp\u003e12.3 Hydrogen\/Deuterium Exchange 296\u003c\/p\u003e \u003cp\u003e12.4 Photochemical Methods 301\u003c\/p\u003e \u003cp\u003e12.5 Implementation of Ion-mobility Spectrometry Coupled with Mass Spectrometry 304\u003c\/p\u003e \u003cp\u003e12.6 Concluding Remarks 305\u003c\/p\u003e \u003cp\u003eReferences 307\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13. Applications of Time-resolved Mass Spectrometry in Biochemical Analysis 315\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e13.1 Enzymatic Reactions 315\u003c\/p\u003e \u003cp\u003e13.1.1 Requirements of Time-resolved Mass Spectrometry in Biocatalysis 315\u003c\/p\u003e \u003cp\u003e13.1.2 Off-line and On-line Methods 316\u003c\/p\u003e \u003cp\u003e13.1.3 Time-resolved Mass Spectrometry Studies of Enzyme Kinetics 317\u003c\/p\u003e \u003cp\u003e13.1.4 Application of Microfluidic Systems 322\u003c\/p\u003e \u003cp\u003e13.1.5 Biochemical Waves 323\u003c\/p\u003e \u003cp\u003e13.2 Time-resolved Mass Spectrometry in Systems and Synthetic Biology 324\u003c\/p\u003e \u003cp\u003e13.3 Monitoring Living Systems 328\u003c\/p\u003e \u003cp\u003e13.3.1 Microbial Samples 328\u003c\/p\u003e \u003cp\u003e13.3.2 Plant and Animal Samples 329\u003c\/p\u003e \u003cp\u003e13.4 Concluding Remarks 330\u003c\/p\u003e \u003cp\u003eReferences 331\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14. Final Remarks 337\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e14.1 Current Progress 337\u003c\/p\u003e \u003cp\u003e14.2 Instrumentation 338\u003c\/p\u003e \u003cp\u003e14.3 Software 339\u003c\/p\u003e \u003cp\u003e14.4 Limitations 340\u003c\/p\u003e \u003cp\u003eReferences 340\u003c\/p\u003e \u003cp\u003eIndex 341\u003c\/p\u003e","brand":"John Wiley \u0026 Sons Inc","offers":[{"title":"Default Title","offer_id":49406940512599,"sku":"9781118887325","price":999.99,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781118887325.jpg?v=1730497629","url":"https:\/\/bookcurl.com\/products\/timeresolved-mass-spectrometry-9781118887325","provider":"Book Curl","version":"1.0","type":"link"}