{"product_id":"the-hplc-ms-handbook-for-practitioners-9783527343072","title":"The HPLC-MS Handbook for Practitioners","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eFilling the gap for an expert text dealing exclusively with the practical aspects of HPLC-MS coupling, this concise, compact, and clear book provides detailed information to enable users to employ the method most efficiently.\u003c\/p\u003e \u003cp\u003eFollowing an overview of the current state of HPLC-MS and its instrumentation, the text goes on to discuss all relevant aspects of method development. A chapter on tips and tricks is followed by user reports on the advantages - and pitfalls - of applying the method in real-life scenarios. The whole is rounded off by a look at future developments by renowned manufacturers.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003ePreface XI\u003c\/p\u003e \u003cp\u003eThe Structure of HPLC-MS for Practitioners XIII\u003c\/p\u003e \u003cp\u003eList of Contributors XV\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart I Overview, Pitfalls, Hardware-Requirements 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 State of the Art in the LC\/MS 3\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eO. Schmitz\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e1.1 Introduction 3\u003c\/p\u003e \u003cp\u003e1.2 Ionization Methods at Atmospheric Pressure 5\u003c\/p\u003e \u003cp\u003e1.2.1 Overview of API Methods 6\u003c\/p\u003e \u003cp\u003e1.2.2 ESI 6\u003c\/p\u003e \u003cp\u003e1.2.3 APCI 8\u003c\/p\u003e \u003cp\u003e1.2.4 APPI 9\u003c\/p\u003e \u003cp\u003e1.2.5 APLI 10\u003c\/p\u003e \u003cp\u003e1.2.6 Determination of Ion Suppression 11\u003c\/p\u003e \u003cp\u003e1.2.7 Best Ionization for Each Question 11\u003c\/p\u003e \u003cp\u003e1.3 Mass Analyzer 12\u003c\/p\u003e \u003cp\u003e1.4 Future Developments 13\u003c\/p\u003e \u003cp\u003e1.5 What Should You Look forWhen Buying a Mass Spectrometer? 14\u003c\/p\u003e \u003cp\u003eReferences 15\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Technical Aspects and Pitfalls of LC\/MS Hyphenation 19\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eM.M. Martin\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2.1 Instrumental Requirements for LC\/MS Analysis – Configuring the Right System for Your Analytical Challenge 20\u003c\/p\u003e \u003cp\u003e2.1.1 (U)HPLC andMass Spectrometry – Not Just a Mere Front-End 20\u003c\/p\u003e \u003cp\u003e2.1.2 UHPLC System Optimization – Gradient Delay and Extra-column Volumes 21\u003c\/p\u003e \u003cp\u003e2.1.3 Does YourMass Spectrometer Fit Your Purpose? 33\u003c\/p\u003e \u003cp\u003e2.1.4 Data Rates and Cycle Times ofModernMass Spectrometers 38\u003c\/p\u003e \u003cp\u003e2.1.5 Complementary Information by Additional Detectors or Mass SpectrometryWon’t Save theWorld 39\u003c\/p\u003e \u003cp\u003e2.2 LC\/MS Method Development and HPLC Method Adaptation – How toMakeMy LC Fit forMS? 43\u003c\/p\u003e \u003cp\u003e2.2.1 Method Development LC\/MS – LC Fits theMS Purposes 44\u003c\/p\u003e \u003cp\u003e2.2.2 Converting Classical HPLC Methods into LC\/MS 53\u003c\/p\u003e \u003cp\u003e2.3 Pitfalls and Error Sources – Sometimes Things Do GoWrong 54\u003c\/p\u003e \u003cp\u003e2.3.1 No Signal at All 54\u003c\/p\u003e \u003cp\u003e2.3.2 Inappropriate Ion Source Settings and Their Impact on the Chromatogram 56\u003c\/p\u003e \u003cp\u003e2.3.3 Ion Suppression 58\u003c\/p\u003e \u003cp\u003e2.3.4 Unknown Mass Signals in the Mass Spectrum 59\u003c\/p\u003e \u003cp\u003e2.3.5 Instrumental Reasons for the Misinterpretation ofMass Spectra 65\u003c\/p\u003e \u003cp\u003e2.4 Conclusion 68\u003c\/p\u003e \u003cp\u003e2.5 Abbreviations 69\u003c\/p\u003e \u003cp\u003eReferences 70\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Aspects of the Development of Methods in LC\/MS Coupling 73\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eT. Teutenberg, T. Hetzel, C. Portner, S. Wiese, C. vom Eyser, and\u003c\/i\u003e\u003ci\u003eJ. Tuerk\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction 73\u003c\/p\u003e \u003cp\u003e3.2 From Target to Screening Analysis 74\u003c\/p\u003e \u003cp\u003e3.2.1 Target Analysis 74\u003c\/p\u003e \u003cp\u003e3.2.2 Suspected-Target Screening 74\u003c\/p\u003e \u003cp\u003e3.2.3 Non-target Screening 74\u003c\/p\u003e \u003cp\u003e3.2.4 Comparable Overview of the Different Acquisition Modes 75\u003c\/p\u003e \u003cp\u003e3.3 The Optimization of Parameters in Chromatography andMass Spectrometry 75\u003c\/p\u003e \u003cp\u003e3.3.1 Requirements and Recommendations for HPLC\/MS Analysis Taking DIN 38407-47 as an Example 75\u003c\/p\u003e \u003cp\u003e3.3.2 The Definition of Critical Peak Pairs in the Context of HPLC\/MS Coupling 77\u003c\/p\u003e \u003cp\u003e3.3.3 The Separation of Polar Components fromthe Column Void Time 79\u003c\/p\u003e \u003cp\u003e3.3.4 Determining the HPLC Method Parameters Using the Example of the Separation of Selected Pharmaceuticals 80\u003c\/p\u003e \u003cp\u003e3.3.5 Carrying out Screening Experiments 84\u003c\/p\u003e \u003cp\u003e3.3.6 Evaluation of the Data and Discussion of the Influencing Parameters 86\u003c\/p\u003e \u003cp\u003e3.3.7 Using Simulation Software for Fine Optimization 98\u003c\/p\u003e \u003cp\u003e3.3.8 Choosing the Stationary Phase Support 99\u003c\/p\u003e \u003cp\u003e3.3.9 The Influence of the Inner Column Diameter and theMobile Phase Flow Rate 103\u003c\/p\u003e \u003cp\u003e3.3.10 The Influence of the Injection Volume 104\u003c\/p\u003e \u003cp\u003e3.3.11 Establishing theMass Spectrometric Parameters 115\u003c\/p\u003e \u003cp\u003e3.3.12 Optimization of theMass Spectrometric Parameters 117\u003c\/p\u003e \u003cp\u003e3.3.13 Quantification Using LC\/MS 122\u003c\/p\u003e \u003cp\u003e3.3.14 Screening Using LC\/MS 128\u003c\/p\u003e \u003cp\u003e3.3.15 Miniaturization – LC\/MS Quo Vadis? 132\u003c\/p\u003e \u003cp\u003eReferences 135\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II Tips, Examples, Trends 139\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 LC\/MS for Everybody\/for Everything? \u003c\/b\u003e\u003cb\u003e–\u003c\/b\u003e\u003cb\u003e LC\/MS Tips 141\u003c\/b\u003e\u003cbr\u003e\u003ci\u003eF. Mandel\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction 141\u003c\/p\u003e \u003cp\u003e4.2 Tip Number 1 142\u003c\/p\u003e \u003cp\u003e4.2.1 Choosing the Right LC\/MS Interface 142\u003c\/p\u003e \u003cp\u003e4.3 Tip Number 2 148\u003c\/p\u003e \u003cp\u003e4.3.1 Which Mobile Phases Are Compatible with LC\/MS? 148\u003c\/p\u003e \u003cp\u003e4.4 Tip Number 3 149\u003c\/p\u003e \u003cp\u003e4.4.1 Phosphate Buffer – The Exception 149\u003c\/p\u003e \u003cp\u003e4.5 Tip Number 4 150\u003c\/p\u003e \u003cp\u003e4.5.1 Paired Ions 150\u003c\/p\u003e \u003cp\u003e4.5.2 Which “Antidote” Is Available? 151\u003c\/p\u003e \u003cp\u003e4.5.3 Summary 152\u003c\/p\u003e \u003cp\u003e4.6 Tip Number 5 152\u003c\/p\u003e \u003cp\u003e4.6.1 Using Additives to Enhance Electrospray Ionization 152\u003c\/p\u003e \u003cp\u003e4.6.2 Additives for APCI 153\u003c\/p\u003e \u003cp\u003e4.6.3 Summary 154\u003c\/p\u003e \u003cp\u003e4.7 Tip Number 6 154\u003c\/p\u003e \u003cp\u003e4.7.1 How Can I Enhance Sensitivity of Detection? 154\u003c\/p\u003e \u003cp\u003e4.8 Tip Number 7 155\u003c\/p\u003e \u003cp\u003e4.8.1 No Linear Response and Poor Dynamic Range? 155\u003c\/p\u003e \u003cp\u003e4.8.2 The Reasons 156\u003c\/p\u003e \u003cp\u003e4.8.3 Possible Solutions 156\u003c\/p\u003e \u003cp\u003e4.8.4 Summary 157\u003c\/p\u003e \u003cp\u003e4.9 Tip Number 8 157\u003c\/p\u003e \u003cp\u003e4.9.1 HowMuch MSn Do I Need? 157\u003c\/p\u003e \u003cp\u003e4.9.2 Solutions 158\u003c\/p\u003e \u003cp\u003e4.9.3 Summary 158\u003c\/p\u003e \u003cp\u003e4.10 Need More Help? 166\u003c\/p\u003e \u003cp\u003eReferences 167\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart III User Reports 169\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 LC Coupled to MS \u003c\/b\u003e\u003cb\u003e–\u003c\/b\u003e\u003cb\u003e a User Report 171\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eA.Muller and A. Hofmann\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eReferences 176\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 ProblemSolvingwithHPLC\/MS\u003c\/b\u003e\u003cb\u003e–\u003c\/b\u003e\u003cb\u003e aPracticalViewfromPractitioners 177\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eE. Fleischer\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction and Scope 177\u003c\/p\u003e \u003cp\u003e6.2 Case Example 1 181\u003c\/p\u003e \u003cp\u003e6.2.1 Investigation ofMethohexital Impurities and Decomposition Products 181\u003c\/p\u003e \u003cp\u003e6.2.2 Sample Preparation 181\u003c\/p\u003e \u003cp\u003e6.3 Case Example 2 183\u003c\/p\u003e \u003cp\u003e6.3.1 Separation of Oligomers from Caprolactam, Multicomponent Separation of Impurities on a Gram Scale 183\u003c\/p\u003e \u003cp\u003e6.4 Case Example 3 184\u003c\/p\u003e \u003cp\u003e6.4.1 Preparation and Isolation of bis-Nalbuphine from Nalbuphine 184\u003c\/p\u003e \u003cp\u003e6.5 Case Example 4 186\u003c\/p\u003e \u003cp\u003e6.5.1 Isolation and Elucidation of Dopamine Impurities 186\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 LC\/MS from the Perspective of a Maintenance Engineer 189\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eO.Muller\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction and Historical Summary 189\u003c\/p\u003e \u003cp\u003e7.2 Spray Techniques 190\u003c\/p\u003e \u003cp\u003e7.3 Passage Through the Ion Path 191\u003c\/p\u003e \u003cp\u003e7.4 The Analyzer 191\u003c\/p\u003e \u003cp\u003e7.5 Maintenance 193\u003c\/p\u003e \u003cp\u003eReferences 198\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart IV Vendor\u003c\/b\u003e\u003cb\u003e’\u003c\/b\u003e\u003cb\u003es Reports 201\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 LC\/MS \u003c\/b\u003e\u003cb\u003e–\u003c\/b\u003e\u003cb\u003e the Past, Present, and Future 203\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eT.L. Sheehan and F. Mandel\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Vendor\u003c\/b\u003e\u003cb\u003e’\u003c\/b\u003e\u003cb\u003es Report \u003c\/b\u003e\u003cb\u003e–\u003c\/b\u003e\u003cb\u003e SCIEX 207\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eD. Schleuder\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Manufacturer Report \u003c\/b\u003e\u003cb\u003e–\u003c\/b\u003e\u003cb\u003e Thermo Fisher Scientific 213\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eM.M. Martin\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10.1 Liquid Chromatography for LC\/MS 214\u003c\/p\u003e \u003cp\u003e10.2 Mass Spectrometry for LC\/MS 215\u003c\/p\u003e \u003cp\u003e10.3 Integrated LC\/MS Solutions 217\u003c\/p\u003e \u003cp\u003e10.4 Software 217\u003c\/p\u003e \u003cp\u003eReferences 219\u003c\/p\u003e \u003cp\u003eAbout the Authors 221\u003c\/p\u003e \u003cp\u003eIndex 227\u003c\/p\u003e","brand":"Wiley-VCH Verlag GmbH","offers":[{"title":"Default Title","offer_id":51021171786071,"sku":"9783527343072","price":70.51,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9783527343072.jpg?v=1750785358","url":"https:\/\/bookcurl.com\/products\/the-hplc-ms-handbook-for-practitioners-9783527343072","provider":"Book Curl","version":"1.0","type":"link"}