{"product_id":"stem-cells-9781118439197","title":"Stem Cells","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e\u003ci\u003eStem Cells: A Short Course\u003c\/i\u003e is a comprehensive text for students delving into the rapidly evolving discipline of stem cell research. Comprised of eight chapters, the text addresses all of the major facets and disciplines related to stem cell biology and research. A brief history of stem cell research serves as an introduction, followed by coverage of stem cell fundamentals; chapters then explore embryonic and fetal amniotic stem cells, adult stem cells, nuclear reprogramming, and cancer stem cells. The book concludes with chapters on stem cell applications, including the role of stem cells in drug discovery and therapeutic applications in spinal cord injury, brain damage, neurological and autoimmune disorders, among others. \u003c\/p\u003e Written by a leader in the field, \u003ci\u003eStem Cells: A Short Course\u003c\/i\u003e appeals to both students and instructors alike, appealing to academic enthusiasm for stem cell research and applications.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003ePreface to the Professor xvii\u003c\/p\u003e \u003cp\u003ePreface to the Student xxi\u003c\/p\u003e \u003cp\u003eAcknowledgments xxiii\u003c\/p\u003e \u003cp\u003eList of Figures xxv\u003c\/p\u003e \u003cp\u003eList of Tables xxxvii\u003c\/p\u003e \u003cp\u003eList of Case Studies xxxix\u003c\/p\u003e \u003cp\u003eList of Focus Boxes xli\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 A HISTORY OF STEM CELL RESEARCH 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eEarly Studies 1\u003c\/p\u003e \u003cp\u003eHematopoietic Stem Cell Discovery 4\u003c\/p\u003e \u003cp\u003eMouse Embryonic Stem Cell Discovery 6\u003c\/p\u003e \u003cp\u003eSuccessful Neural Stem Cell Culture 7\u003c\/p\u003e \u003cp\u003eThe Discovery of Cancer Stem Cells 8\u003c\/p\u003e \u003cp\u003eHuman Embryonic Stem Cell Discovery 9\u003c\/p\u003e \u003cp\u003eStem Cells And Cloning 11\u003c\/p\u003e \u003cp\u003eCord Blood Embryonic]Like Stem Cells—An Alternative to Es and Adult Stem Cells 14\u003c\/p\u003e \u003cp\u003eBreakthrough In Spinal Cord Injury Repair 15\u003c\/p\u003e \u003cp\u003eThe Generation of ips Cells 16\u003c\/p\u003e \u003cp\u003eiPS Cells Derived from Keratinocytes 20\u003c\/p\u003e \u003cp\u003eiPS Induction Without the Use of Viruses 20\u003c\/p\u003e \u003cp\u003eTransposon]Mediated iPS 21\u003c\/p\u003e \u003cp\u003eProtein]Based iPS 22\u003c\/p\u003e \u003cp\u003eThe Discovery of Human Amniotic Stem Cells 24\u003c\/p\u003e \u003cp\u003eHuman Embryonic Stem Cells Generated Without Embryo Destruction 25\u003c\/p\u003e \u003cp\u003eHuman Cloning 25\u003c\/p\u003e \u003cp\u003eMesenchymal Stem Cell]Derived Human Knee Cartilage 27\u003c\/p\u003e \u003cp\u003eThe First Clinical Trial Using Human Embryonic Stem Cells 28\u003c\/p\u003e \u003cp\u003eMitochondrial DNA: A Barrier To Autologous Cell Therapeutics 29\u003c\/p\u003e \u003cp\u003eInduced Pluripotency And The Potential To Save Endangered Species 30\u003c\/p\u003e \u003cp\u003eChapter Summary 33\u003c\/p\u003e \u003cp\u003eKey Terms 37\u003c\/p\u003e \u003cp\u003eReview Questions 39\u003c\/p\u003e \u003cp\u003eThought Question 40\u003c\/p\u003e \u003cp\u003eSuggested Readings 40\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 FUNDAMENTALS OF STEM CELLS 43\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eBasic in Vitro Cell Culture—A Historical Perspective 43\u003c\/p\u003e \u003cp\u003eStem Cell Culture—Optimal Conditions and Techniques 48\u003c\/p\u003e \u003cp\u003eEmbryonic Stem Cell Culture 49\u003c\/p\u003e \u003cp\u003eHematopoietic Stem Cell Culture 52\u003c\/p\u003e \u003cp\u003eNotch Regulation of HSC Proliferation 52\u003c\/p\u003e \u003cp\u003eOther Drivers of HSC Proliferation 53\u003c\/p\u003e \u003cp\u003eAdipose]Derived Stem Cell Culture 54\u003c\/p\u003e \u003cp\u003eThe Study of Embryonic Development 56\u003c\/p\u003e \u003cp\u003eEmbryonic Development and the Origin of Stem Cells 56\u003c\/p\u003e \u003cp\u003eEarly Events in Embryogenesis 56\u003c\/p\u003e \u003cp\u003eGerm Cell Development 61\u003c\/p\u003e \u003cp\u003eBasic Properties of Stem Cells 63\u003c\/p\u003e \u003cp\u003eLong]Term Self]Renewal 63\u003c\/p\u003e \u003cp\u003eDifferent Potency Capabilities 63\u003c\/p\u003e \u003cp\u003eTotipotency 64\u003c\/p\u003e \u003cp\u003ePluripotency 65\u003c\/p\u003e \u003cp\u003eMultipotency 68\u003c\/p\u003e \u003cp\u003eOligopotency 68\u003c\/p\u003e \u003cp\u003eUnipotency 69\u003c\/p\u003e \u003cp\u003eTypes of Stem Cells 70\u003c\/p\u003e \u003cp\u003eEmbryonic Stem Cells 70\u003c\/p\u003e \u003cp\u003eFetal Stem Cells 70\u003c\/p\u003e \u003cp\u003eAmniotic Stem Cells 71\u003c\/p\u003e \u003cp\u003eAdult Stem Cells 71\u003c\/p\u003e \u003cp\u003eInduced Pluripotency (iPS) Cells 71\u003c\/p\u003e \u003cp\u003eCancer Stem Cells 71\u003c\/p\u003e \u003cp\u003eThe Potential of Stem Cells in Medicine and Medical Research 71\u003c\/p\u003e \u003cp\u003eTherapeutics 71\u003c\/p\u003e \u003cp\u003eTissue Engineering 71\u003c\/p\u003e \u003cp\u003eCell Therapy 73\u003c\/p\u003e \u003cp\u003eCell]Based Drug Screening 75\u003c\/p\u003e \u003cp\u003eChapter Summary 77\u003c\/p\u003e \u003cp\u003eKey Terms 80\u003c\/p\u003e \u003cp\u003eReview Questions 83\u003c\/p\u003e \u003cp\u003eThought Question 84\u003c\/p\u003e \u003cp\u003eSuggested Readings 85\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 EMBRYONIC STEM, FETAL, AND AMNIOTIC STEM CELLS 87\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eES Cells 87\u003c\/p\u003e \u003cp\u003eBasic Properties 87\u003c\/p\u003e \u003cp\u003ePluripotency 87\u003c\/p\u003e \u003cp\u003eIndefinite Replicative Capacity 89\u003c\/p\u003e \u003cp\u003eSignaling and Transcriptional Control of ES Cell Replication 90\u003c\/p\u003e \u003cp\u003eExamples of ES Cells 92\u003c\/p\u003e \u003cp\u003eMouse ES Cells 92\u003c\/p\u003e \u003cp\u003eRat ES Cells 95\u003c\/p\u003e \u003cp\u003eNonhuman Primate ES Cells 97\u003c\/p\u003e \u003cp\u003eHuman ES Cells 100\u003c\/p\u003e \u003cp\u003eEC Cells 103\u003c\/p\u003e \u003cp\u003eEmbryonal Germ Cells 105\u003c\/p\u003e \u003cp\u003eEG Cell Growth Factor Signaling 105\u003c\/p\u003e \u003cp\u003eComparing Embryonically Derived Cells 106\u003c\/p\u003e \u003cp\u003eFetal Stem Cells 108\u003c\/p\u003e \u003cp\u003eBasic Properties 108\u003c\/p\u003e \u003cp\u003eAmniotic Fluid Stem Cells 108\u003c\/p\u003e \u003cp\u003eWharton’s Jelly Stem Cells 109\u003c\/p\u003e \u003cp\u003eAmniotic Membrane Stem Cells 110\u003c\/p\u003e \u003cp\u003ePlacental Stem Cells 110\u003c\/p\u003e \u003cp\u003eChapter Summary 112\u003c\/p\u003e \u003cp\u003eKey Terms 114\u003c\/p\u003e \u003cp\u003eReview Questions 115\u003c\/p\u003e \u003cp\u003eThought Question 116\u003c\/p\u003e \u003cp\u003eSuggested Reading 116\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 ADULT STEM CELLS 118\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eDiscovery and Origin of ASCs 118\u003c\/p\u003e \u003cp\u003eBasic Properties of ASCs 118\u003c\/p\u003e \u003cp\u003eSelf]Renewal 119\u003c\/p\u003e \u003cp\u003eMultipotency 119\u003c\/p\u003e \u003cp\u003eExamples of ASCs 120\u003c\/p\u003e \u003cp\u003eHematopoietic Stem Cells 122\u003c\/p\u003e \u003cp\u003eMorphology and Marker Expression 123\u003c\/p\u003e \u003cp\u003eSources 123\u003c\/p\u003e \u003cp\u003eSignaling and Multipotency 123\u003c\/p\u003e \u003cp\u003eMuscle]Derived Stem Cells 127\u003c\/p\u003e \u003cp\u003eMyosatellite Cell Morphology and Marker Expression 129\u003c\/p\u003e \u003cp\u003eSources 129\u003c\/p\u003e \u003cp\u003eSignaling, Transcriptional Control, and Multipotency 129\u003c\/p\u003e \u003cp\u003eAdipose]Derived Stem Cells 130\u003c\/p\u003e \u003cp\u003eMorphology and Marker Expression 130\u003c\/p\u003e \u003cp\u003eSources 131\u003c\/p\u003e \u003cp\u003eSignaling and Multipotency 132\u003c\/p\u003e \u003cp\u003eMesenchymal Stem Cells 134\u003c\/p\u003e \u003cp\u003eMorphology and Marker Expression 134\u003c\/p\u003e \u003cp\u003eSources 136\u003c\/p\u003e \u003cp\u003eSignaling and Multipotency 136\u003c\/p\u003e \u003cp\u003eNeural Stem Cells 140\u003c\/p\u003e \u003cp\u003eMorphology and Marker Expression 141\u003c\/p\u003e \u003cp\u003eSources and Origins 143\u003c\/p\u003e \u003cp\u003eSignaling and Multipotency 146\u003c\/p\u003e \u003cp\u003eEndothelial Stem Cells 150\u003c\/p\u003e \u003cp\u003eMorphology and Marker Expression 150\u003c\/p\u003e \u003cp\u003eSources and Origins 152\u003c\/p\u003e \u003cp\u003eSignaling and Multipotency 153\u003c\/p\u003e \u003cp\u003eChapter Summary 156\u003c\/p\u003e \u003cp\u003eKey Terms 158\u003c\/p\u003e \u003cp\u003eReview Questions 161\u003c\/p\u003e \u003cp\u003eThought Question 162\u003c\/p\u003e \u003cp\u003eSuggested Readings 162\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 NUCLEAR REPROGRAMMING 164\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eExamples of Nuclear Reprogramming in Nature 166\u003c\/p\u003e \u003cp\u003eCell Fusion 166\u003c\/p\u003e \u003cp\u003eCell Fusion for the Generation of Hybridomas 169\u003c\/p\u003e \u003cp\u003eMechanisms of Cell Fusion 172\u003c\/p\u003e \u003cp\u003eComparison of Cell Fusion Techniques 173\u003c\/p\u003e \u003cp\u003eElectrofusion 173\u003c\/p\u003e \u003cp\u003ePegylation 173\u003c\/p\u003e \u003cp\u003eViral Induction 173\u003c\/p\u003e \u003cp\u003eMechanism of Nuclear Reprogramming in Cell Fusion 175\u003c\/p\u003e \u003cp\u003eSomatic Cell Nuclear Transfer 176\u003c\/p\u003e \u003cp\u003eMethod for the Production of SCNT]Derived Cells 177\u003c\/p\u003e \u003cp\u003eSomatic Cell Nuclear Transfer for the Creation of Stem Cells 178\u003c\/p\u003e \u003cp\u003eBasic Properties of SCNT]Derived Stem Cells 180\u003c\/p\u003e \u003cp\u003eExamples of SCNT]Derived Stem Cells 181\u003c\/p\u003e \u003cp\u003eA Note Regarding Genomic Abnormalities in SCNT]Derived Clones 182\u003c\/p\u003e \u003cp\u003eTelomeric Length 182\u003c\/p\u003e \u003cp\u003eDNA Methylation and Epigenetics 183\u003c\/p\u003e \u003cp\u003eX]Chromosome Inactivation 185\u003c\/p\u003e \u003cp\u003eInduced Pluripotency 185\u003c\/p\u003e \u003cp\u003eBreakthrough in the Production of iPS Cells 187\u003c\/p\u003e \u003cp\u003eMethods for the Production of iPS Cells 191\u003c\/p\u003e \u003cp\u003eRetroviral and Lentiviral Gene Delivery 192\u003c\/p\u003e \u003cp\u003eAdenoviral Gene Delivery 195\u003c\/p\u003e \u003cp\u003eSendai Viral Gene Delivery 196\u003c\/p\u003e \u003cp\u003ePlasmid]Based Gene Delivery 196\u003c\/p\u003e \u003cp\u003emRNA Delivery 198\u003c\/p\u003e \u003cp\u003eMicroRNA Induction 199\u003c\/p\u003e \u003cp\u003eTransposon Delivery 200\u003c\/p\u003e \u003cp\u003eDirect Protein Delivery 200\u003c\/p\u003e \u003cp\u003eBasic Properties of iPS Cells 201\u003c\/p\u003e \u003cp\u003eA General Comparison of iPS Cells and Embryonic Stem Cells 203\u003c\/p\u003e \u003cp\u003eExamples of Derived iPS Cells 205\u003c\/p\u003e \u003cp\u003eMouse Embryonic Fibroblast]Derived iPS Cells 206\u003c\/p\u003e \u003cp\u003eHuman Adult Skin]Derived iPS Cells 209\u003c\/p\u003e \u003cp\u003eAdvantages of ips Cells Over Other Cell Types 210\u003c\/p\u003e \u003cp\u003eOrigin and Bioethics 211\u003c\/p\u003e \u003cp\u003ePatient Specificity 211\u003c\/p\u003e \u003cp\u003eBroad Lineage Differentiation Potential 211\u003c\/p\u003e \u003cp\u003e“Unlimited” Supply 211\u003c\/p\u003e \u003cp\u003eEase of Generation 212\u003c\/p\u003e \u003cp\u003eChapter Summary 212\u003c\/p\u003e \u003cp\u003eKey Terms 214\u003c\/p\u003e \u003cp\u003eReview Questions 217\u003c\/p\u003e \u003cp\u003eThought Question 218\u003c\/p\u003e \u003cp\u003eSuggested Readings 218\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 CANCER STEM CELLS 220\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eBackground on The Origins of Cancer 220\u003c\/p\u003e \u003cp\u003eDiscovery and Origin of Cancer Stem Cells 221\u003c\/p\u003e \u003cp\u003eBasic Properties of Cancer Stem Cells 225\u003c\/p\u003e \u003cp\u003eA Comparison of Cancer Stem Cells and Normal Stem Cells 226\u003c\/p\u003e \u003cp\u003eSignaling Pathways Involved in Cancer Stem Cell Transformation 228\u003c\/p\u003e \u003cp\u003eExamples of Cancer Stem Cells 229\u003c\/p\u003e \u003cp\u003eBreast 229\u003c\/p\u003e \u003cp\u003eCentral Nervous System 229\u003c\/p\u003e \u003cp\u003eColon 231\u003c\/p\u003e \u003cp\u003eOvary 233\u003c\/p\u003e \u003cp\u003ePancreas 233\u003c\/p\u003e \u003cp\u003eProstate 234\u003c\/p\u003e \u003cp\u003eMelanoma 235\u003c\/p\u003e \u003cp\u003eMultiple Myeloma 237\u003c\/p\u003e \u003cp\u003eStrategies for Treatment Targeting Cancer Stem Cells 238\u003c\/p\u003e \u003cp\u003eMelanoma Treatment Targeting Chimeric Antigen Receptor 238\u003c\/p\u003e \u003cp\u003eMultiple Myeloma Treatment Targeting CD20 239\u003c\/p\u003e \u003cp\u003eChapter Summary 240\u003c\/p\u003e \u003cp\u003eKey Terms 241\u003c\/p\u003e \u003cp\u003eReview Questions 243\u003c\/p\u003e \u003cp\u003eThought Question 244\u003c\/p\u003e \u003cp\u003eSuggested Readings 244\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 STEM CELLS AS DRUG DISCOVERY PLATFORMS 245\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eEmbryonic Stem Cells and Mouse Models of Gene Function 245\u003c\/p\u003e \u003cp\u003eStem Cell]Based Screening Assays 250\u003c\/p\u003e \u003cp\u003eStem Cells as Lineage Resources for HTS 250\u003c\/p\u003e \u003cp\u003eEmbryonic Stem Cells as a Resource 251\u003c\/p\u003e \u003cp\u003eAdult Stem Cells as a Resource 253\u003c\/p\u003e \u003cp\u003eiPS Cells as a Resource 254\u003c\/p\u003e \u003cp\u003eCancer Stem Cell Screens 256\u003c\/p\u003e \u003cp\u003eReprogramming Screens 258\u003c\/p\u003e \u003cp\u003eAnalysis of Disease Pathways 261\u003c\/p\u003e \u003cp\u003eStem Cells As A Toxicity]Testing Platform 267\u003c\/p\u003e \u003cp\u003eStem Cells as a Resource for Developmental Toxicity Testing 267\u003c\/p\u003e \u003cp\u003eStem Cells as a Source for Post]Natal Environmental Toxicity Testing 268\u003c\/p\u003e \u003cp\u003eCardiotoxicity 269\u003c\/p\u003e \u003cp\u003eHepatotoxicity 272\u003c\/p\u003e \u003cp\u003eChapter Summary 273\u003c\/p\u003e \u003cp\u003eKey Terms 274\u003c\/p\u003e \u003cp\u003eReview Questions 276\u003c\/p\u003e \u003cp\u003eThought Question 277\u003c\/p\u003e \u003cp\u003eSuggested Readings 277\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 THERAPEUTIC APPLICATIONS OF STEM CELLS 279\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eHistory of Stem Cells as Therapeutics 279\u003c\/p\u003e \u003cp\u003eHistory of Tissue Engineering 279\u003c\/p\u003e \u003cp\u003eDisease]Specific Treatment and Patient Trials 282\u003c\/p\u003e \u003cp\u003eStem Cell]Based Patient Trials: An Overview 282\u003c\/p\u003e \u003cp\u003eCardiomyopathy and Cardiovascular Disease (CV) 284\u003c\/p\u003e \u003cp\u003eNeuropathies and Neurodegenerative Diseases 286\u003c\/p\u003e \u003cp\u003eSpinal Cord Injury 286\u003c\/p\u003e \u003cp\u003eBrain Damage 288\u003c\/p\u003e \u003cp\u003eParkinson’s Disease 292\u003c\/p\u003e \u003cp\u003eAutoimmune Disorders 292\u003c\/p\u003e \u003cp\u003eCorneal Defects 296\u003c\/p\u003e \u003cp\u003eHematopoietic Disorders 297\u003c\/p\u003e \u003cp\u003eSickle Cell Disease 297\u003c\/p\u003e \u003cp\u003eWiskott–Aldrich Syndrome 299\u003c\/p\u003e \u003cp\u003eCancer 300\u003c\/p\u003e \u003cp\u003eMuscular Dystrophy 301\u003c\/p\u003e \u003cp\u003eLiver Disorders 303\u003c\/p\u003e \u003cp\u003eVeterinary Applications 306\u003c\/p\u003e \u003cp\u003eEquine 307\u003c\/p\u003e \u003cp\u003eCanine 309\u003c\/p\u003e \u003cp\u003eOsteoarthritis (OA) 309\u003c\/p\u003e \u003cp\u003eMyocardial Infarction 311\u003c\/p\u003e \u003cp\u003eSpinal Cord Injury 313\u003c\/p\u003e \u003cp\u003eStem Cells as an Emerging Industry 314\u003c\/p\u003e \u003cp\u003eSeminal Discoveries Driving the Growth of a New Industry 317\u003c\/p\u003e \u003cp\u003eRegulation and Reimbursement of Stem Cell Commercialization 320\u003c\/p\u003e \u003cp\u003eA Word about Induced Pluripotency and Commercialization 320\u003c\/p\u003e \u003cp\u003eChapter Summary 321\u003c\/p\u003e \u003cp\u003eKey Terms 324\u003c\/p\u003e \u003cp\u003eReview Questions 326\u003c\/p\u003e \u003cp\u003eThought Question 327\u003c\/p\u003e \u003cp\u003eSuggested Readings 328\u003c\/p\u003e \u003cp\u003eAbout the Author 330\u003c\/p\u003e \u003cp\u003eIndex 331\u003c\/p\u003e","brand":"John Wiley and Sons Ltd","offers":[{"title":"Default Title","offer_id":49406870225239,"sku":"9781118439197","price":68.36,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781118439197.jpg?v=1730497394","url":"https:\/\/bookcurl.com\/products\/stem-cells-9781118439197","provider":"Book Curl","version":"1.0","type":"link"}