{"product_id":"rna-detection-methods-and-protocols-1649-methods-in-molecular-biology-9781493984183","title":"RNA Detection Methods and Protocols 1649 Methods","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eThis volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e\u003ci\u003ePreface…\u003c\/i\u003e\u003cbr\u003e \u003cb\u003eTable of Contents…\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eContributing Authors…\u003c\/i\u003e\u003cbr\u003e\u003c\/p\u003e\u003cb\u003e\u003cbr\u003e1. \u003c\/b\u003e\u003cb\u003eThe Secret Life of RNA: Lessons from Emerging Methodologies\u003cbr\u003e\u003c\/b\u003eCaroline Medioni and Florence Besse\u003cbr\u003e\u003cb\u003e2.\u003c\/b\u003e \u003cb\u003eQuantification of 2'-O-Me Residues in RNA using Next-Generation Sequencing (Illumina RiboMethSeq Protocol)\u003cbr\u003e\u003c\/b\u003eLilia Ayadi, Yuri Motorin, and Virginie Marchand\u003cbr\u003e\u003cbr\u003e\u003cb\u003e3.\u003c\/b\u003e \u003cb\u003eIdentifying the m\u003csup\u003e6\u003c\/sup\u003eA Methylome by Affinity Purification and Sequencing\u003cbr\u003e\u003c\/b\u003ePhillip J. Hsu and Chuan He\u003cbr\u003e\u003cbr\u003e\u003cb\u003e4.\u003c\/b\u003e \u003cb\u003ePARIS: Psoralen Analysis of RNA Interactions and Structures with High Throughput and Resolution\u003cbr\u003e\u003c\/b\u003eZhipeng Lu, Jing Gong, and Qiangfeng Cliff Zhang\u003cbr\u003e\u003cbr\u003e\u003cb\u003e5.\u003c\/b\u003e \u003cb\u003eAxon-TRAP-RiboTag: Affinity-Purification of Translated mRNAs from Neuronal Axons in Mouse \u003ci\u003eIn Vivo\u003cbr\u003e\u003c\/i\u003e\u003c\/b\u003eToshiaki Shigeoka, Jane Jung, Christine E. Holt, and Hosung Jung\u003cbr\u003e\u003cbr\u003e\u003cb\u003e6.\u003c\/b\u003e \u003cb\u003eLCM-Seq: A Method for Spatial Transcriptomic Profiling using Laser Capture Microdissection Coupled with PolyA-Based RNA Sequencing\u003cbr\u003e\u003c\/b\u003eSusanne Nichterwitz, Julio Aguila Benitez, Rein Hoogstraaten, Qiaolin Deng, and Eva Hedlund\u003cbr\u003e\u003cbr\u003e\u003cb\u003e7.\u003c\/b\u003e \u003cb\u003eSpatial Transcriptomics: Constructing a Single-Cell Resolution Transcriptome-Wide Expression Atlas\u003cbr\u003e\u003c\/b\u003eKaia Achim, Hernando Martínez Vergara, and Jean-Baptiste Pettit\u003cbr\u003e\u003cbr\u003e\u003cb\u003e8.\u003c\/b\u003e \u003cb\u003eSingle mRNA Molecule Detection in \u003ci\u003eDrosophila\u003cbr\u003e\u003c\/i\u003e\u003c\/b\u003eShawn C. Little and Thomas Gregor\u003cbr\u003e\u003cbr\u003e\u003cb\u003e9.\u003c\/b\u003e \u003cb\u003eDetection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embryos\u003cbr\u003e\u003c\/b\u003eL. Carine Stapel, Coleman Broaddus, and Nadine L. Vastenhouw\u003cbr\u003e\u003cbr\u003e\u003cb\u003e10.\u003c\/b\u003e  \u003cb\u003eSuper-Resolution Single Molecule FISH at the \u003ci\u003eDrosophila\u003c\/i\u003e Neuromuscular Junction\u003cbr\u003e\u003c\/b\u003eJoshua S. Titlow, Lu Yang, Richard M. Parton, Ana Palanca, and Ilan Davis\u003cbr\u003e\u003cbr\u003e\u003cb\u003e11.\u003c\/b\u003e  Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections\u003cbr\u003eCatherine Rabouille\u003cbr\u003e\u003cbr\u003e\u003cb\u003e12.\u003c\/b\u003e  \u003cb\u003eHybridization Chain Reaction for Direct mRNA Detection without Nucleic Acid Purification\u003cbr\u003e\u003c\/b\u003eYao Xu and Zhi Zheng\u003cbr\u003e\u003cbr\u003e\u003cb\u003e13.\u003c\/b\u003e  \u003cb\u003e\u003ci\u003eIn Situ\u003c\/i\u003e Detection of MicroRNA Expression with RNAscope Probes\u003cbr\u003e\u003c\/b\u003eViravuth P. Yin\u003cbr\u003e\u003cbr\u003e\u003cb\u003e14.\u003c\/b\u003e  \u003cb\u003ePadlock Probes to Detect Single Nucleotide Polymorphisms\u003cbr\u003e\u003c\/b\u003eTomasz Krzywkowski and Mats Nilsson\u003cbr\u003e\u003cbr\u003e\u003cb\u003e15.\u003c\/b\u003e  \u003cb\u003eQuantifying Gene Expression in Living Cells with Ratiometric Bimolecular Beacons\u003cbr\u003e\u003c\/b\u003eYantao Yang, Mingming Chen, Christopher K. Krueger, Andrew Tsourkas, and Antony K. Chen\u003cbr\u003e\u003cbr\u003e\u003cb\u003e16.\u003c\/b\u003e  \u003cb\u003eOptimizing Molecular Beacons for Intracellular Analysis of RNA\u003cbr\u003e\u003c\/b\u003eMingming Chen, Yantao Yang, Christopher K. Krueger, and Antony K. Chen\u003cbr\u003e\u003cbr\u003e\u003cb\u003e17.\u003c\/b\u003e  \u003cb\u003eLive Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH\u003cbr\u003e\u003c\/b\u003eDan Ohtan Wang\u003cbr\u003e\u003cbr\u003e\u003cb\u003e18.\u003c\/b\u003e  \u003cb\u003e\u003ci\u003eIn Vivo\u003c\/i\u003e Visualization and Function Probing of Transport mRNPs using Injected FIT Probes\u003cbr\u003e\u003c\/b\u003eJasmine Chamiolo, Imre Gaspar, Anne Ephrussi, and Oliver Seitz\u003cbr\u003e\u003cbr\u003e\u003cb\u003e19.  Visualizing RNA in Live Bacterial Cells using Fluorophore- and Quencher-Binding Aptamers\u003c\/b\u003e\u003cbr\u003eMurat Sunbul, Ankita Arora, and Andres Jäschke\u003cbr\u003e\u003cbr\u003e\u003cb\u003e20.\u003c\/b\u003e  \u003cb\u003eMethod for Imaging Live-Cell RNA using an RNA Aptamer and a Fluorescent Probe\u003cbr\u003e\u003c\/b\u003eShin-ichi Sato, Kenji Yatsuzuka, Yousuke Katsuda, and Motonari Uesugi\u003cbr\u003e\u003cbr\u003e\u003cb\u003e21.\u003c\/b\u003e  \u003cb\u003eRNA Live Imaging in the Model Microorganism \u003ci\u003eUstilago maydis\u003cbr\u003e\u003c\/i\u003e\u003c\/b\u003eS. Zander, K. Müntjes, and M. Feldbrügge\u003cbr\u003e\u003cbr\u003e\u003cb\u003e22.\u003c\/b\u003e  \u003cb\u003eReal-Time Fluorescence Imaging of Single-Molecule Endogenous Non-Coding RNA in Living Cells\u003cbr\u003e\u003c\/b\u003eHideaki Yoshimura and Takeaki Ozawa\u003cbr\u003e\u003cbr\u003e\u003cb\u003e23.\u003c\/b\u003e  \u003cb\u003eLive Imaging of mRNA Synthesis in \u003ci\u003eDrosophila\u003cbr\u003e\u003c\/i\u003e\u003c\/b\u003eHernan G. Garcia and Thomas Gregor\u003cbr\u003e\u003cbr\u003e\u003cb\u003e24.\u003c\/b\u003e  \u003cb\u003eImaging Newly Transcribed RNA in Cells by Using a Clickable Azide-Modified UTP Analog\u003cbr\u003e\u003c\/b\u003eAnupam A. Sawant, Sanjeev Galande, and Seergazhi G. Srivatsan\u003cbr\u003e\u003cbr\u003e\u003cb\u003e25.  \u003c\/b\u003e\u003cb\u003eDetection of the First Round of Translation: The TRICK Assay\u003cbr\u003e\u003c\/b\u003eFranka Voigt, Jan Eglinger, and Jeffrey A. Chao\u003cbr\u003e\u003cbr\u003e\u003cb\u003e26.  \u003c\/b\u003e\u003cb\u003eImaging Translation Dynamics of Single mRNA Molecules in Live Cells\u003cbr\u003e\u003c\/b\u003eSuzan Ruijtenberg, Tim A. Hoek, Xiaowei Yan, and Marvin E. Tanenbaum\u003cbr\u003e\u003cbr\u003e\u003cb\u003e27.  \u003c\/b\u003e\u003cb\u003eSystematic Detection of Poly(A)\u003csup\u003e+\u003c\/sup\u003e RNA-Interacting Proteins and Their Differential Binding\u003cbr\u003e\u003c\/b\u003eMiha Milek and Markus Landthaler\u003cbr\u003e\u003cbr\u003e\u003cb\u003e28.  \u003c\/b\u003e\u003cb\u003eIsolation and Characterization of Endogenous RNPs from Brain Tissues\u003cbr\u003e\u003c\/b\u003eRico Schieweck, Foong yee Ang, Renate Fritzsche, and Michael A. Kiebler\u003cbr\u003e\u003cbr\u003e\u003cb\u003e29.  \u003c\/b\u003e\u003cb\u003eIndividual Nucleotide Resolution UV Cross-Linking and Immunoprecipitation (iCLIP) to Determine Protein-RNA Interactions\u003cbr\u003e\u003c\/b\u003eChristopher R. Sibley\u003cbr\u003e\u003cbr\u003e\u003cb\u003e30.\u003c\/b\u003e  \u003cb\u003eRNA Tagging: Preparation of High-Throughput Sequencing Libraries\u003cbr\u003e\u003c\/b\u003eChristopher P. Lapointe and Marvin Wickens\u003cbr\u003e\u003cb\u003e\u003cbr\u003e31.  \u003c\/b\u003e\u003cb\u003eRAP-MS: A Method to Identify Proteins that Interact Directly with a Specific RNA Molecule in Cells\u003cbr\u003e\u003c\/b\u003eColleen A. McHugh and Mitchell Guttman\u003cp\u003e\u003c\/p\u003e","brand":"Springer New York","offers":[{"title":"Default Title","offer_id":51040590594391,"sku":"9781493984183","price":125.99,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781493984183.jpg?v=1750947211","url":"https:\/\/bookcurl.com\/products\/rna-detection-methods-and-protocols-1649-methods-in-molecular-biology-9781493984183","provider":"Book Curl","version":"1.0","type":"link"}