{"product_id":"regulatory-tcells-9781071626467","title":"Regulatory TCells","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eThis volume provides methods and models for investigating the immunosuppressive subset of CD4+ T-cells, regulatory T-cells (Treg). Chapters guide readers through, basic protocols for analyzing Treg in mice and humans, single-cell analysis for analyzing Treg, and methods for the analysis of Treg in clinical application. Written in the format of the highly successful \u003ci\u003eMethods in Molecular Biology \u003c\/i\u003eseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003eAuthoritative and cutting-edge, \u003ci\u003eRegulatory T-Cells: Methods and Protocols \u003c\/i\u003eaims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. \u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003ePart I     Immunological techniques for analysing Treg\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e1.       Analysis of the in vivo function of follicular regulatory T (TFR) cells in the regulation of antibody response\u003c\/p\u003e  \u003cp\u003eLin Luo and Jianmei W. Leavenworth\u003c\/p\u003e  \u003cp\u003e2.       Analysis of peripherally derived Treg in the intestines\u003c\/p\u003e  \u003cp\u003eTadashi Takeuchi and Hiroshi Ohno\u003c\/p\u003e  \u003cp\u003e3.       Isolation and analysis of tumor infiltrating Treg\u003c\/p\u003e  \u003cp\u003eKatharina Bergerhoff\u003c\/p\u003e  \u003cp\u003e4.       Induction of peripheral Treg\u003c\/p\u003e  José Almeida-Santos, Marie-Louise Bergman, and Jocelyne Demengeot\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e5.       Adoptive transfer and bone marrow chimera models to analyse Treg function and differentiation       \u003c\/p\u003e  \u003cp\u003eVital da Silva Domingues, Iris Caramalho, Marie-Louise Bergman, and Jocelyne Demengeot\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003ePart III         Molecular techniques for analysing Treg\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e6.       Functional analysis of Foxp3 and its mutants by retroviral transduction of murine primary CD4\u003csup\u003e+\u003c\/sup\u003e T cells\u003c\/p\u003e  \u003cp\u003e Akira Nakajima, Ryuichi Murakami, Shohei Hori\u003c\/p\u003e  \u003cp\u003e7.       Genetic Tools for Analyzing Foxp3\u003csup\u003e+\u003c\/sup\u003e Treg cells: Fluorochrome-Based Transcriptional Reporters and Genetic Fate-Mapping\u003c\/p\u003e  \u003cp\u003eMario Simonetti, Acelya Yilmazer, and Karsten Kretschmer\u003c\/p\u003e  \u003cp\u003e8.       Depletion of Treg by the Diphtheria Toxin System\u003c\/p\u003e  \u003cp\u003eSho Hanakawa, Zachary Chow, and Kenji Kabashima\u003c\/p\u003e  9.       Analysis of FOXP3 DNA methylation patterns to identify functional FOXP3+ T-Cell subpopulations\u003cp\u003e\u003c\/p\u003e  \u003cp\u003eEkaterina Minskaia and João F. Lacerda\u003c\/p\u003e  \u003cp\u003e10.   Protein Ubiquitination Assay\u003c\/p\u003e  \u003cp\u003ePing Wei, Rui Liang, and Fan Pan\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003ePart IV          Human Treg analysis and clinical application\u003c\/b\u003e\u003c\/p\u003e  11.   Phenotypic and Functional Studies of Human Treg cell Subpopulations\u003cp\u003e\u003c\/p\u003e  \u003cp\u003eMo Atif, Mustapha Cherai, Makoto Miyara\u003c\/p\u003e  \u003cp\u003e12.   \u003ci\u003eIn vitro \u003c\/i\u003eanalysis of CTLA-4 mediated transendocytosis by Regulatory T cells\u003c\/p\u003e  \u003cp\u003eErin Waters, Cayman Williams, Alan Kennedy, and David M Sansom\u003c\/p\u003e  \u003cp\u003e13.   Isolation and functional characterisation of regulatory CD4+ T cells from the inflamed joints of patients with rheumatoid arthritis\u003c\/p\u003e  \u003cp\u003eGiovanni A.M. Povoleri, Veerle Fleskens and Leonie S. Taams\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e14.   \u003c\/b\u003eA GMP protocol for the manufacture of regulatory T cells for clinical application\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003eAlice O’Neil, Matthew Brook, Seetha Abdul-Wahab, Joanna Hester, Giovanna Lombardi and Fadi Issa\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003ePart V Single cell and high-dimensional techniques for analysing Treg\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e15.   Regulatory T-cell phenotyping using CyTOF\u003c\/p\u003e  \u003cp\u003eHugo Barcenilla, Mikael Pihl, Florence Sjögren, Louise Magnusson, and Rosaura Casas\u003c\/p\u003e  \u003cp\u003e16.   High-dimensional single-cell profiling of tumor-infiltrating CD4+ regulatory T cells\u003c\/p\u003e  \u003cp\u003eGiorgia Alvisi, Simone Puccio, Rahul Roychoudhuri, Caterina Scirgolea and Enrico Lugli\u003c\/p\u003e  \u003cp\u003e17.   Single-cell transcriptome analysis of Treg\u003c\/p\u003e  \u003cp\u003eBenjy Tan Jek Yang, Masahiro Ono, and Yorifumi Satou\u003c\/p\u003e","brand":"Springer-Verlag New York Inc.","offers":[{"title":"Default Title","offer_id":49406772150615,"sku":"9781071626467","price":116.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781071626467.jpg?v=1730497055","url":"https:\/\/bookcurl.com\/products\/regulatory-tcells-9781071626467","provider":"Book Curl","version":"1.0","type":"link"}