{"product_id":"protein-engineering-9781493984633","title":"Protein Engineering","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eThis volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e1. Protein Engineering - Past, Present, and Future \u003cp\u003eStefan Lutz and Samantha M. Iamurri\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003ePart I\u003c\/p\u003e  \u003cp\u003e2. (Semi)rational Protein Design\u003c\/p\u003e  \u003cp\u003eIvan V. Korendovych\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e3. Computational Analysis of Protein Tunnels and Channels\u003c\/p\u003e  \u003cp\u003eJan Brezovsky, Barbora Kozlikova, and Jiri Damborsky\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e4. YASARA - A Tool to Obtain Structural Guidance in Biocatalytic Investigations\u003c\/p\u003e  \u003cp\u003eHenrik Land and Maria Svedendahl Humble\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e5. A Computational Library Design Protocol for Rapid Improvement of Protein Stability - FRESCO\u003c\/p\u003e  \u003cp\u003eHein J. Wijma, Maximilian J.L.J. Fürst, and Dick B. Janssen\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e6. Directed Evolution of Proteins Based on Mutational Scanning\u003c\/p\u003e  \u003cp\u003eCarlos G. Acevedo-Rocha, Matteo Ferla, and Manfred T. Reetz\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003ePart II\u003c\/p\u003e  \u003cp\u003e7. A Brief Guide to the High-throughput Expression of Directed Evolution Libraries\u003c\/p\u003e  \u003cp\u003eAna Luísa Ribeiro, Mario Mencía, and Aurelio Hidalgo\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e8. Library Growth and Protein Expression: Optimal and Reproducible Microtiter Plate Expression of Recombinant Enzymes in \u003ci\u003eE. coli\u003c\/i\u003e using MTP Shakers\u003c\/p\u003e  \u003cp\u003eSandy Schmidt, Mark Dörr, and Uwe T. Bornscheuer\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e9. Normalized Screening of Protein Engineering Libraries by Split-GFP Crude Cell Extract Quantification\u003c\/p\u003e  \u003cp\u003eJavier Santos-Aberturas, Mark Dörr, and Uwe T. Bornscheuer\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e10. Functional Analysis of Membrane Proteins Produced by Cell-Free Translation\u003c\/p\u003e  \u003cp\u003eSrujan Kumar Dondapati, Doreen A Wüstenhagen, and Stefan Kubick\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003ePART III\u003c\/p\u003e  \u003cp\u003e11. Practical Considerations Regarding the Choice of the Best High-throughput Assay\u003c\/p\u003e  \u003cp\u003eCarolin Mügge and Robert Kouristb\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e12. High-throughput Screening Assays for Lipolytic Enzymes\u003c\/p\u003e  \u003cp\u003eAlexander Fulton, Marc R. Hayes, Ulrich Schwaneberg, Jörg Pietruszka, and Karl-Erich Jaeger\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e13. Continuous High-throughput Colorimetric Assays for α-transaminases\u003c\/p\u003e  \u003cp\u003eEgon Heuson, Jean-Louis Petit, Franck Charmantray, Véronique de Bérardinis, and Thierry Gefflaut\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e14. Colorimetric High-troughput Screening Assays for the Directed Evolution of Fungal Laccases\u003c\/p\u003e  \u003cp\u003eIsabel Pardo and Susana Camarero\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e15. Directed Co-evolution of Two Cellulosic Enzymes in \u003ci\u003eEscherichia coli\u003c\/i\u003e Based on their Synergistic Reactions\u003c\/p\u003e  \u003cp\u003eMin Liu, Lidan Ye, and Hongwei Yu\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e16. Program-guided Design of High-throughput Enzyme Screening Experiments and Automated Data Analysis\/Evaluation \u003c\/p\u003e  \u003cp\u003eMark Dörr and Uwe T. Bornscheuer\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e17. Solid Phase Agar Plate Assay for Screening Amine Transaminases\u003c\/p\u003e  \u003cp\u003eMartin S. Weiß, Uwe T. Bornscheuer, and Matthias Höhne\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e18. Ultrahigh-throughput Screening of Single-cell Lysates for Directed Evolution and Functional Metagenomics\u003c\/p\u003e  \u003cp\u003eFabrice Gielen, Pierre-Yves Colin, Philip Mair, and Florian Hollfelder\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e19. Isolation of pH-sensitive Antibody Fragments by Fluorescence-activated Cell Sorting and Yeast Surface Display \u003ci\u003e\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003eChristian Schröter, Simon Krah, Jan Beck, Doreen Könning, Julius Grzeschik, Bernhard Valldorf, Stefan Zielonka, and Harald Kolmar\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e20. Library Generation and Auxotrophic Selection Assays in \u003ci\u003eEscherichia coli\u003c\/i\u003e and \u003ci\u003eThermus thermophilus\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003eJörg Claren, Thomas Schwab, and Reinhard Sterner\u003c\/p\u003e","brand":"Humana Press Inc.","offers":[{"title":"Default Title","offer_id":49409211269463,"sku":"9781493984633","price":59.99,"currency_code":"GBP","in_stock":false}],"url":"https:\/\/bookcurl.com\/products\/protein-engineering-9781493984633","provider":"Book Curl","version":"1.0","type":"link"}