{"product_id":"preservation-of-cells-9781118989845","title":"Preservation of Cells","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eHelps those that use cell preservation to develop new protocols or improve existing protocols\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eThis book provides readers with the tools needed to develop or debug a preservation protocol for cells. The core structure and content of the text grew from a professional short course that has been offered at the Biopreservation Core Resource for the last 10 years. This comprehensive text describes, step by step, the individual elements of a protocol, including the relevant scientific principles for each phase of the protocol. It can be used by anyone who is involved in cell preservationeven by those who are not experts in freezing of cellsbecause it provides the scientific basis for those that want to understand the basis for the protocol.\u003c\/p\u003e \u003cp\u003e\u003ci\u003ePreservation of Cells: A Practical Manual\u003c\/i\u003e begins by first introducing readers to the subject of preserving cells. It then goes on to cover Pre-freeze Processing and Characterization; Formulation and Introduction of Cryopreser\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003ePreface xiii\u003c\/p\u003e \u003cp\u003eAcknowledgments xvii\u003c\/p\u003e \u003cp\u003eNomenclature xix\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Introduction 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eMammalian Cells: Modern Workhorses 1\u003c\/p\u003e \u003cp\u003eProducts from Cells 1\u003c\/p\u003e \u003cp\u003eCells as Therapeutic Agents 2\u003c\/p\u003e \u003cp\u003eBiomarkers for Health or Disease 2\u003c\/p\u003e \u003cp\u003eIn Vitro Models 3\u003c\/p\u003e \u003cp\u003eBridging the Gap 3\u003c\/p\u003e \u003cp\u003eThe Preservation Toolkit 5\u003c\/p\u003e \u003cp\u003eHypothermic Storage 5\u003c\/p\u003e \u003cp\u003eCryopreservation 6\u003c\/p\u003e \u003cp\u003eVitrification 7\u003c\/p\u003e \u003cp\u003eDry State Storage 8\u003c\/p\u003e \u003cp\u003eFit]for]Purpose 8\u003c\/p\u003e \u003cp\u003eOne Size Does Not Fit All 9\u003c\/p\u003e \u003cp\u003eThe Process is the Product 9\u003c\/p\u003e \u003cp\u003eReproducibility 12\u003c\/p\u003e \u003cp\u003eSafety 12\u003c\/p\u003e \u003cp\u003eDispelling the Myth of the Cold Black Box 12\u003c\/p\u003e \u003cp\u003eReferences 13\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Pre]freeze Processing and Characterization 15\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003ePre]freeze Processing 15\u003c\/p\u003e \u003cp\u003eDigestion of Cells from Intact Tissue 15\u003c\/p\u003e \u003cp\u003eHypothermic Storage 16\u003c\/p\u003e \u003cp\u003eSelection of Subpopulations 17\u003c\/p\u003e \u003cp\u003eActivation or Stimulation 18\u003c\/p\u003e \u003cp\u003eGenetic Modification 18\u003c\/p\u003e \u003cp\u003eCulture 19\u003c\/p\u003e \u003cp\u003ePre]freeze Process Monitoring 19\u003c\/p\u003e \u003cp\u003ePre]freeze Characterization 20\u003c\/p\u003e \u003cp\u003eIdentity 20\u003c\/p\u003e \u003cp\u003eGenetic Stability 21\u003c\/p\u003e \u003cp\u003eEnumeration 21\u003c\/p\u003e \u003cp\u003ePurity 22\u003c\/p\u003e \u003cp\u003eAdventitious Agents 22\u003c\/p\u003e \u003cp\u003eMicrobial Testing of Cell Therapy Products 23\u003c\/p\u003e \u003cp\u003eSpecial Considerations for the Characterization of Cell Therapies 24\u003c\/p\u003e \u003cp\u003eAnnotation of Pre]freeze Processing 24\u003c\/p\u003e \u003cp\u003eScientific Principles 25\u003c\/p\u003e \u003cp\u003ePutting Principles into Action 25\u003c\/p\u003e \u003cp\u003eReferences 26\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Formulation and Introduction of Cryopreservation Solutions 29\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eImportance of Cryoprotective Agents 29\u003c\/p\u003e \u003cp\u003eMechanisms of Cryoprotection 31\u003c\/p\u003e \u003cp\u003eFormulating a Cryopreservation Solution 31\u003c\/p\u003e \u003cp\u003eFormulation of a Vitrification Solution 33\u003c\/p\u003e \u003cp\u003eCharacterization and Quality Control for Cryoprotective Solutions 34\u003c\/p\u003e \u003cp\u003eToxicity of CPAs 35\u003c\/p\u003e \u003cp\u003eOsmotic Toxicity 35\u003c\/p\u003e \u003cp\u003eBiochemical Toxicity 36\u003c\/p\u003e \u003cp\u003eDeveloping a Protocol for Introducing CPA Solutions 37\u003c\/p\u003e \u003cp\u003eThe Basic Experiment 37\u003c\/p\u003e \u003cp\u003eIntroduction of Vitrification Solutions 38\u003c\/p\u003e \u003cp\u003eCell Concentration 39\u003c\/p\u003e \u003cp\u003eRemoval of CPA Solution 40\u003c\/p\u003e \u003cp\u003eSafety Considerations for Cryopreservation Solutions 40\u003c\/p\u003e \u003cp\u003eCryopreservation Containers 41\u003c\/p\u003e \u003cp\u003eOverwraps 42\u003c\/p\u003e \u003cp\u003eLabeling 43\u003c\/p\u003e \u003cp\u003eSample Annotation 44\u003c\/p\u003e \u003cp\u003eScientific Principles 44\u003c\/p\u003e \u003cp\u003ePutting Principles into Practice 44\u003c\/p\u003e \u003cp\u003eReferences 44\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Freezing Protocols 47\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eImportance of Cooling Rate 47\u003c\/p\u003e \u003cp\u003eControlled]rate Freezing 48\u003c\/p\u003e \u003cp\u003eControlled Cooling]rate Protocols 49\u003c\/p\u003e \u003cp\u003eSegment 1: Initial Hold Period 49\u003c\/p\u003e \u003cp\u003eSegment 2: Cooling 50\u003c\/p\u003e \u003cp\u003eUncontrolled Nucleation 53\u003c\/p\u003e \u003cp\u003eManual Nucleation 54\u003c\/p\u003e \u003cp\u003eAutomatic Nucleation 54\u003c\/p\u003e \u003cp\u003eVerifying Segment 2 (Including S2a) 55\u003c\/p\u003e \u003cp\u003e“Delayed” Latent Heat 55\u003c\/p\u003e \u003cp\u003eSegment 3 56\u003c\/p\u003e \u003cp\u003eVerifying Segment 3 56\u003c\/p\u003e \u003cp\u003eOther Types of Controlled]rate Protocols 57\u003c\/p\u003e \u003cp\u003ePassive Freezing 57\u003c\/p\u003e \u003cp\u003eTransfer to Storage 59\u003c\/p\u003e \u003cp\u003eVitrification 60\u003c\/p\u003e \u003cp\u003eIndependent Temperature Measurement 60\u003c\/p\u003e \u003cp\u003eScientific Principles 61\u003c\/p\u003e \u003cp\u003ePutting Principles into Practice 62\u003c\/p\u003e \u003cp\u003eReferences 62\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Storage and Shipping of Frozen Cells 65\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eScientific Basis for Selection of a Storage Temperature 65\u003c\/p\u003e \u003cp\u003eAdditional Considerations for Vitrified Samples 67\u003c\/p\u003e \u003cp\u003eStandards, Guidelines, and Best Practices 67\u003c\/p\u003e \u003cp\u003eFacilities 68\u003c\/p\u003e \u003cp\u003eStorage Equipment and Environment 69\u003c\/p\u003e \u003cp\u003eMapping Storage Devices and Setting Alarm Limits 70\u003c\/p\u003e \u003cp\u003eMonitoring Systems 71\u003c\/p\u003e \u003cp\u003eSafety 71\u003c\/p\u003e \u003cp\u003eInventory Management System 72\u003c\/p\u003e \u003cp\u003eStability in Storage 72\u003c\/p\u003e \u003cp\u003eTemperature Fluctuations 73\u003c\/p\u003e \u003cp\u003eInfluence of Background Ionizing Radiation on Stability in Storage 74\u003c\/p\u003e \u003cp\u003eShelf]Life of Samples in Storage 75\u003c\/p\u003e \u003cp\u003eFit]for]Purpose Storage Practices 75\u003c\/p\u003e \u003cp\u003eRisk Mitigation in Long]Term Storage 76\u003c\/p\u003e \u003cp\u003eShipping or Transport of Cells 76\u003c\/p\u003e \u003cp\u003eGeneral Shipping Considerations 77\u003c\/p\u003e \u003cp\u003eLiquid Nitrogen Dry Shippers 78\u003c\/p\u003e \u003cp\u003eTemperature Mapping of a Shipper 79\u003c\/p\u003e \u003cp\u003ePackaging of Samples Being Shipped 79\u003c\/p\u003e \u003cp\u003eMonitoring of Shipments 79\u003c\/p\u003e \u003cp\u003eResponsibilities 79\u003c\/p\u003e \u003cp\u003eSample Annotation 80\u003c\/p\u003e \u003cp\u003eScientific Principles 80\u003c\/p\u003e \u003cp\u003ePutting Principles into Practice 81\u003c\/p\u003e \u003cp\u003eReferences 81\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Thawing and Post]Thaw Processing 85\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eThawing\u003c\/p\u003e \u003cp\u003eEquipment 86\u003c\/p\u003e \u003cp\u003eTransporting Samples Prior to Thawing 87\u003c\/p\u003e \u003cp\u003eEstimating Your Thawing Rate 87\u003c\/p\u003e \u003cp\u003eThawing and Infusion of Cell Therapy Products 89\u003c\/p\u003e \u003cp\u003eSafety Considerations for Thawing 90\u003c\/p\u003e \u003cp\u003ePost]Thaw Processing 90\u003c\/p\u003e \u003cp\u003ePost]Thaw Washing 90\u003c\/p\u003e \u003cp\u003eDilution 91\u003c\/p\u003e \u003cp\u003eInfusion of Cells Immediately Post]Thaw 91\u003c\/p\u003e \u003cp\u003eRemoval of Vitrification Solutions 92\u003c\/p\u003e \u003cp\u003eWash Solutions 92\u003c\/p\u003e \u003cp\u003eScientific Principles 94\u003c\/p\u003e \u003cp\u003ePutting Principles into Practice 94\u003c\/p\u003e \u003cp\u003eReferences 94\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Post]Thaw Assessment 97\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eCommon Measures Used in Post]Thaw Assessment 98\u003c\/p\u003e \u003cp\u003ePhysical Integrity 98\u003c\/p\u003e \u003cp\u003eMetabolic Activity 99\u003c\/p\u003e \u003cp\u003eMechanical Activity 100\u003c\/p\u003e \u003cp\u003eMitotic Activity 101\u003c\/p\u003e \u003cp\u003eDifferentiation Potential 102\u003c\/p\u003e \u003cp\u003eTransplantation Potential 103\u003c\/p\u003e \u003cp\u003eStrategies to Improve the Accuracy and Reproducibility of Post]Thaw\u003c\/p\u003e \u003cp\u003eAssessment 103\u003c\/p\u003e \u003cp\u003eEliminate Measurement Bias 103\u003c\/p\u003e \u003cp\u003eCompensating for Post]Thaw Apoptosis 105\u003c\/p\u003e \u003cp\u003ePost]Thaw Assessment Using a Single Measure 106\u003c\/p\u003e \u003cp\u003eOptical Methods of Post]Thaw Assessment 106\u003c\/p\u003e \u003cp\u003eRelease Criteria 107\u003c\/p\u003e \u003cp\u003eScientific Principles 107\u003c\/p\u003e \u003cp\u003ePutting Principles into Practice 107\u003c\/p\u003e \u003cp\u003eReferences 108\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Algorithm]Driven Protocol Optimization 111\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eSmall Cell Number\/High Throughput Approach 113\u003c\/p\u003e \u003cp\u003eValidating Operation of the Algorithm 114\u003c\/p\u003e \u003cp\u003eFlexibility 115\u003c\/p\u003e \u003cp\u003ePractical\u003c\/p\u003e \u003cp\u003eNotes 115\u003c\/p\u003e \u003cp\u003eModeling\u003c\/p\u003e \u003cp\u003ein Cryobiology 115\u003c\/p\u003e \u003cp\u003eReferences 116\u003c\/p\u003e \u003cp\u003eProtocols Introduction 117\u003c\/p\u003e \u003cp\u003eProtocol\u003c\/p\u003e \u003cp\u003eContributors 118\u003c\/p\u003e \u003cp\u003eCryopreservation of Endothelial Cells in Suspension 119\u003c\/p\u003e \u003cp\u003ePrinciple 119\u003c\/p\u003e \u003cp\u003eEquipment and Supplies 119\u003c\/p\u003e \u003cp\u003eEquipment 119\u003c\/p\u003e \u003cp\u003eSupplies 120\u003c\/p\u003e \u003cp\u003eSafety 120\u003c\/p\u003e \u003cp\u003eProcedure 121\u003c\/p\u003e \u003cp\u003eCell Preparation 121\u003c\/p\u003e \u003cp\u003ePreparation of Cryoprotectant Solution 121\u003c\/p\u003e \u003cp\u003eUsing Powdered HES 122\u003c\/p\u003e \u003cp\u003eUsing Pentastarch Solution 122\u003c\/p\u003e \u003cp\u003eCryoprotectant Addition 122\u003c\/p\u003e \u003cp\u003eFreezing 122\u003c\/p\u003e \u003cp\u003eControlled]rate Freezing with a Methanol Bath 122\u003c\/p\u003e \u003cp\u003eAlternative Freezing Procedure 123\u003c\/p\u003e \u003cp\u003eThawing 123\u003c\/p\u003e \u003cp\u003eExpected Results 123\u003c\/p\u003e \u003cp\u003eReferences 123\u003c\/p\u003e \u003cp\u003eCryopreservation of Peripheral Blood Mononuclear Cells from Whole Blood 125\u003c\/p\u003e \u003cp\u003ePrinciple 125\u003c\/p\u003e \u003cp\u003eProtocol 1: Isolation of PBMCS Directly over Ficoll]Hypaque 125\u003c\/p\u003e \u003cp\u003eEquipment 125\u003c\/p\u003e \u003cp\u003eMaterials 126\u003c\/p\u003e \u003cp\u003eReagents 126\u003c\/p\u003e \u003cp\u003eProcedure 126\u003c\/p\u003e \u003cp\u003eProtocol 2: Isolation of PBMCS Using SepMates 127\u003c\/p\u003e \u003cp\u003eEquipment 127\u003c\/p\u003e \u003cp\u003eMaterials 128\u003c\/p\u003e \u003cp\u003eReagents 128\u003c\/p\u003e \u003cp\u003eProcedure 128\u003c\/p\u003e \u003cp\u003eAppendix A Human Serum AB Freezing Media 129\u003c\/p\u003e \u003cp\u003eMaterials 129\u003c\/p\u003e \u003cp\u003eEquipment 130\u003c\/p\u003e \u003cp\u003eReagents 130\u003c\/p\u003e \u003cp\u003eProcedure 130\u003c\/p\u003e \u003cp\u003eCryopreservation of Human Adipose Stem Cells 131\u003c\/p\u003e \u003cp\u003ePrinciple 131\u003c\/p\u003e \u003cp\u003eEquipment and Supplies 131\u003c\/p\u003e \u003cp\u003eReagents and Media 132\u003c\/p\u003e \u003cp\u003eProcedure 133\u003c\/p\u003e \u003cp\u003eIsolation of Human ASCs from Lipoaspirate 133\u003c\/p\u003e \u003cp\u003eMagnetic Cell Sorting (Optional) 135\u003c\/p\u003e \u003cp\u003eCryopreservation 135\u003c\/p\u003e \u003cp\u003eControlled]rate Freezing of Human ASCs 135\u003c\/p\u003e \u003cp\u003eThawing Human ASCs 137\u003c\/p\u003e \u003cp\u003eNotes 138\u003c\/p\u003e \u003cp\u003eReference 139\u003c\/p\u003e \u003cp\u003eCryopreservation of Red Blood Cells 141\u003c\/p\u003e \u003cp\u003eMethod I: High Glycerol\/Slow Cooling Technique (Meryman and Hornblower 1972) 141\u003c\/p\u003e \u003cp\u003ePreparation of the RBC Concentrate 141\u003c\/p\u003e \u003cp\u003eAddition of the Cryoprotective Solution 141\u003c\/p\u003e \u003cp\u003eCooling 142\u003c\/p\u003e \u003cp\u003eRewarming 142\u003c\/p\u003e \u003cp\u003eRemoval of the Cryoprotectant and Debris 142\u003c\/p\u003e \u003cp\u003eMethod II: A Low Glycerol\/Rapid Cooling Technique (Rowe, Eyster, and Kellner 1968) 143\u003c\/p\u003e \u003cp\u003eMethod III: Hydroxyethylstarch\/Rapid Cooling Technique (Sputtek 2007) 144\u003c\/p\u003e \u003cp\u003eReferences 146\u003c\/p\u003e \u003cp\u003eCryopreservation of Oocytes by Slow Freezing 147\u003c\/p\u003e \u003cp\u003ePrinciple 147\u003c\/p\u003e \u003cp\u003eSpecimen Requirements 147\u003c\/p\u003e \u003cp\u003eEquipment and Supplies Needed 147\u003c\/p\u003e \u003cp\u003eEquipment 147\u003c\/p\u003e \u003cp\u003eSupplies 148\u003c\/p\u003e \u003cp\u003eProcedure 148\u003c\/p\u003e \u003cp\u003eSafety 152\u003c\/p\u003e \u003cp\u003eCalculations 152\u003c\/p\u003e \u003cp\u003eReporting Results 152\u003c\/p\u003e \u003cp\u003eProcedure Notes 153\u003c\/p\u003e \u003cp\u003eLimitations of Procedure 153\u003c\/p\u003e \u003cp\u003eOocyte Vitrification and Warming 155\u003c\/p\u003e \u003cp\u003ePrinciple 155\u003c\/p\u003e \u003cp\u003eEquipment and Supplies 155\u003c\/p\u003e \u003cp\u003eEquipment 155\u003c\/p\u003e \u003cp\u003eSupplies 155\u003c\/p\u003e \u003cp\u003eProcedure 156\u003c\/p\u003e \u003cp\u003eQuality Control 160\u003c\/p\u003e \u003cp\u003eSafety 161\u003c\/p\u003e \u003cp\u003eTransportation of Hematopoietic Progenitor Cells and Other Cellular Products 163\u003c\/p\u003e \u003cp\u003ePrinciple\/Rationale 163\u003c\/p\u003e \u003cp\u003eSpecimen 163\u003c\/p\u003e \u003cp\u003eEquipment\/Reagents 163\u003c\/p\u003e \u003cp\u003eQuality Control 164\u003c\/p\u003e \u003cp\u003eProcedure 164\u003c\/p\u003e \u003cp\u003eAdditional Information 165\u003c\/p\u003e \u003cp\u003eFurther Reading 165\u003c\/p\u003e \u003cp\u003eCryopreservation of Hematopoietic Progenitor Cells 167\u003c\/p\u003e \u003cp\u003ePrinciple\/Rationale 167\u003c\/p\u003e \u003cp\u003eProtocol\/Processing Schema 168\u003c\/p\u003e \u003cp\u003eSpecimen 168\u003c\/p\u003e \u003cp\u003eEquipment\/Reagents 168\u003c\/p\u003e \u003cp\u003eQualityControl 169\u003c\/p\u003e \u003cp\u003eProcedure169\u003c\/p\u003e \u003cp\u003eAppendix A Alternate Cryopreservation\u003c\/p\u003e \u003cp\u003eHarness Set]2 or 4 Bags 173\u003c\/p\u003e \u003cp\u003eFurther Reading 173\u003c\/p\u003e \u003cp\u003eThawing of Hematopoietic Progenitor Cells 175\u003c\/p\u003e \u003cp\u003ePrinciple\/Rationale 175\u003c\/p\u003e \u003cp\u003eEquipment\/Reagents 175\u003c\/p\u003e \u003cp\u003eQualityControl 176\u003c\/p\u003e \u003cp\u003eProcedure176\u003c\/p\u003e \u003cp\u003eFurther Reading 177\u003c\/p\u003e \u003cp\u003eProcessing and Cryopreservation of T]Cells 179\u003c\/p\u003e \u003cp\u003ePrinciple\/Rationale 179\u003c\/p\u003e \u003cp\u003eProtocol\/Processing Schema: N\/A 179\u003c\/p\u003e \u003cp\u003eSpecimens179\u003c\/p\u003e \u003cp\u003eEquipment\/Reagents 179\u003c\/p\u003e \u003cp\u003eQuality Control 180\u003c\/p\u003e \u003cp\u003eProcedure 180\u003c\/p\u003e \u003cp\u003eFurther Reading 182\u003c\/p\u003e \u003cp\u003eThawing and Reinfusion of Cryopreserved T]Cells 183\u003c\/p\u003e \u003cp\u003ePrinciple\/Rationale 183\u003c\/p\u003e \u003cp\u003eProtocol\/Processing Schema 183\u003c\/p\u003e \u003cp\u003eSpecimen 184\u003c\/p\u003e \u003cp\u003eEquipment\/Reagents 184\u003c\/p\u003e \u003cp\u003eQuality Control 184\u003c\/p\u003e \u003cp\u003eProcedure 184\u003c\/p\u003e \u003cp\u003eFurther Reading 187\u003c\/p\u003e \u003cp\u003eIndex 189\u003c\/p\u003e","brand":"John Wiley and Sons Ltd","offers":[{"title":"Default Title","offer_id":49406963188055,"sku":"9781118989845","price":75.56,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781118989845.jpg?v=1730497711","url":"https:\/\/bookcurl.com\/products\/preservation-of-cells-9781118989845","provider":"Book Curl","version":"1.0","type":"link"}