{"product_id":"practical-highperformance-liquid-chromatography-9780470682180","title":"Practical HighPerformance Liquid Chromatography","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eThis textbook aims to present the knowledge which is necessary for everyday HPLC measurements to be made, in a detailed and easy-to-follow manner. It contains a wealth of practical material and covers a wide range of subjects.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003ePreface to the Fifth Edition xiii\u003c\/p\u003e \u003cp\u003eImportant and Useful Equations for HPLC 1 vii\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Introduction 5\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 HPLC: A powerful separation method 5\u003c\/p\u003e \u003cp\u003e1.2 A first HPLC experiment 5\u003c\/p\u003e \u003cp\u003e1.3 Liquid chromatographic separation modes 8\u003c\/p\u003e \u003cp\u003e1.4 The HPLC instrument 9\u003c\/p\u003e \u003cp\u003e1.5 Safety in the HPLC laboratory 10\u003c\/p\u003e \u003cp\u003e1.6 Comparison between high-performance liquid chromatography and gas chromatography 11\u003c\/p\u003e \u003cp\u003e1.7 Comparison between high-performance liquid chromatography and capillary electrophoresis 12\u003c\/p\u003e \u003cp\u003e1.8 Units for pressure, length and viscosity 13\u003c\/p\u003e \u003cp\u003e1.9 Scientific journals 14\u003c\/p\u003e \u003cp\u003e1.10 Recommended books 15\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Theoretical Principles 17\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 The chromatographic process 17\u003c\/p\u003e \u003cp\u003e2.2 Band broadening 19\u003c\/p\u003e \u003cp\u003e2.3 The chromatogram and its purport 23\u003c\/p\u003e \u003cp\u003e2.4 Graphical representation of peak pairs with different degree of resolution 30\u003c\/p\u003e \u003cp\u003e2.5 Factors affecting resolution 35\u003c\/p\u003e \u003cp\u003e2.6 Extra-column volumes (dead volumes) 40\u003c\/p\u003e \u003cp\u003e2.7 Tailing 41\u003c\/p\u003e \u003cp\u003e2.8 Peak capacity and statistical resolution probability 46\u003c\/p\u003e \u003cp\u003e2.9 Effects of temperature in HPLC 49\u003c\/p\u003e \u003cp\u003e2.10 The limits of HPLC 51\u003c\/p\u003e \u003cp\u003e2.11 How to obtain peak capacity 55\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Pumps 59\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 General requirements 59\u003c\/p\u003e \u003cp\u003e3.2 The short-stroke piston pump 59\u003c\/p\u003e \u003cp\u003e3.3 Maintenance and repair 62\u003c\/p\u003e \u003cp\u003e3.4 Other pump designs 63\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Preparation of Equipment up to Sample Injection 65\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Selection of the mobile phase 65\u003c\/p\u003e \u003cp\u003e4.2 Preparation of the mobile phase 67\u003c\/p\u003e \u003cp\u003e4.3 Gradient systems 68\u003c\/p\u003e \u003cp\u003e4.4 Capillary tubing 70\u003c\/p\u003e \u003cp\u003e4.5 Fittings 72\u003c\/p\u003e \u003cp\u003e4.6 Sample injectors 74\u003c\/p\u003e \u003cp\u003e4.7 Sample solution and sample volume 78\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Solvent Properties 81\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Table of organic solvents 81\u003c\/p\u003e \u003cp\u003e5.2 Solvent selectivity 83\u003c\/p\u003e \u003cp\u003e5.3 Miscibility 83\u003c\/p\u003e \u003cp\u003e5.4 Buffers 84\u003c\/p\u003e \u003cp\u003e5.5 Shelf life of mobile phases. 87\u003c\/p\u003e \u003cp\u003e5.6 The mixing cross 88\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Detectors 91\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 General 91\u003c\/p\u003e \u003cp\u003e6.2 UV detectors 96\u003c\/p\u003e \u003cp\u003e6.3 Refractive index detectors 99\u003c\/p\u003e \u003cp\u003e6.4 Fluorescence detectors 101\u003c\/p\u003e \u003cp\u003e6.5 Electrochemical (amperometric) detectors 103\u003c\/p\u003e \u003cp\u003e6.6 Light-scattering detectors 104\u003c\/p\u003e \u003cp\u003e6.7 Other detectors 106\u003c\/p\u003e \u003cp\u003e6.8 Multiple detection 107\u003c\/p\u003e \u003cp\u003e6.9 Indirect detection 108\u003c\/p\u003e \u003cp\u003e6.10 Coupling with spectroscopy 109\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Columns and Stationary Phases 117\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Columns for HPLC 117\u003c\/p\u003e \u003cp\u003e7.2 Precolumns 119\u003c\/p\u003e \u003cp\u003e7.3 General properties of stationary phases 120\u003c\/p\u003e \u003cp\u003e7.4 Silica 125\u003c\/p\u003e \u003cp\u003e7.5 Chemically modified silica 126\u003c\/p\u003e \u003cp\u003e7.6 Styrene-divinylbenzene 129\u003c\/p\u003e \u003cp\u003e7.7 Some other stationary phases 133\u003c\/p\u003e \u003cp\u003e7.8 Column care and regeneration 136\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 HPLC Column Tests 141\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Simple tests for HPLC columns 141\u003c\/p\u003e \u003cp\u003e8.2 Determination of particle size 143\u003c\/p\u003e \u003cp\u003e8.3 Determination of breakthrough time 144\u003c\/p\u003e \u003cp\u003e8.4 The test mixture 146\u003c\/p\u003e \u003cp\u003e8.5 Dimensionless parameters for HPLC column characterization 148\u003c\/p\u003e \u003cp\u003e8.6 The van Deemter equation from reduced parameters and its use in column diagnosis 152\u003c\/p\u003e \u003cp\u003e8.7 van Deemter curves and other coherences 153\u003c\/p\u003e \u003cp\u003e8.8 Diffusion coefficients 155\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Adsorption Chromatography: Normal-Phase Chromatography 159\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 What is adsorption? 159\u003c\/p\u003e \u003cp\u003e9.2 The eluotropic series 162\u003c\/p\u003e \u003cp\u003e9.3 Selectivity properties of the mobile phase 165\u003c\/p\u003e \u003cp\u003e9.4 Choice and optimization of the mobile phase 166\u003c\/p\u003e \u003cp\u003e9.5 Applications 168\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Reversed-Phase Chromatography 173\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Principle 173\u003c\/p\u003e \u003cp\u003e10.2 Mobile phases in reversed-phase chromatography 174\u003c\/p\u003e \u003cp\u003e10.3 Solvent selectivity and strength 177\u003c\/p\u003e \u003cp\u003e10.4 Stationary phases 181\u003c\/p\u003e \u003cp\u003e10.5 Method development in reversed-phase chromatography 185\u003c\/p\u003e \u003cp\u003e10.6 Applications 188\u003c\/p\u003e \u003cp\u003e10.7 Hydrophobic interaction chromatography 191\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Chromatography with Chemically Bonded Phases 195\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction 195\u003c\/p\u003e \u003cp\u003e11.2 Properties of some stationary phases 195\u003c\/p\u003e \u003cp\u003e11.3 Hydrophilic interaction chromatography 200\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Ion-Exchange Chromatography 203\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction 203\u003c\/p\u003e \u003cp\u003e12.2 Principle 203\u003c\/p\u003e \u003cp\u003e12.3 Properties of ion exchangers 204\u003c\/p\u003e \u003cp\u003e12.4 Influence of the mobile phase 207\u003c\/p\u003e \u003cp\u003e12.5 Special possibilities of ion exchange 208\u003c\/p\u003e \u003cp\u003e12.6 Practical hints 210\u003c\/p\u003e \u003cp\u003e12.7 Applications 213\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Ion-Pair Chromatography 217\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction 217\u003c\/p\u003e \u003cp\u003e13.2 Ion-pair chromatography in practice 218\u003c\/p\u003e \u003cp\u003e13.3 Applications 220\u003c\/p\u003e \u003cp\u003e13.4 Appendix: UV detection using ion-pair reagents 221\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Ion Chromatography 225\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e14.1 Principle 225\u003c\/p\u003e \u003cp\u003e14.2 Suppression techniques 226\u003c\/p\u003e \u003cp\u003e14.3 Phase systems 226\u003c\/p\u003e \u003cp\u003e14.4 Applications 230\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Size-Exclusion Chromatography 231\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e15.1 Principle 231\u003c\/p\u003e \u003cp\u003e15.2 The calibration chromatogram 234\u003c\/p\u003e \u003cp\u003e15.3 Molecular mass determination by means of size-exclusion chromatography 238\u003c\/p\u003e \u003cp\u003e15.4 Coupled size-exclusion columns 241\u003c\/p\u003e \u003cp\u003e15.5 Phase systems 243\u003c\/p\u003e \u003cp\u003e15.6 Applications 244\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16 Affinity Chromatography. 249\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e16.1 Principle 249\u003c\/p\u003e \u003cp\u003e16.2 Affinity chromatography as a special case of HPLC 251\u003c\/p\u003e \u003cp\u003e16.3 Applications 252\u003c\/p\u003e \u003cp\u003e\u003cb\u003e17 Choice of Method 255\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e17.1 The various possibilities 255\u003c\/p\u003e \u003cp\u003e17.2 Method transfer 260\u003c\/p\u003e \u003cp\u003e\u003cb\u003e18 Solving the Elution Problem 263\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e18.1 The elution problem 263\u003c\/p\u003e \u003cp\u003e18.2 Solvent gradients 264\u003c\/p\u003e \u003cp\u003e18.3 Column switching 270\u003c\/p\u003e \u003cp\u003e18.4 Comprehensive two-dimensional HPLC 272\u003c\/p\u003e \u003cp\u003e18.5 Optimization of an isocratic chromatogram using four solvents 273\u003c\/p\u003e \u003cp\u003e18.6 Optimization of the other parameters 276\u003c\/p\u003e \u003cp\u003e18.7 Mixed stationary phases 284\u003c\/p\u003e \u003cp\u003e\u003cb\u003e19 Analytical HPLC 285\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e19.1 Qualitative analysis 285\u003c\/p\u003e \u003cp\u003e19.2 Trace analysis 287\u003c\/p\u003e \u003cp\u003e19.3 Quantitative analysis 291\u003c\/p\u003e \u003cp\u003e19.4 Recovery 296\u003c\/p\u003e \u003cp\u003e19.5 Peak-height and peak-area determination for quantitative analysis 299\u003c\/p\u003e \u003cp\u003e19.6 Integration errors 303\u003c\/p\u003e \u003cp\u003e19.7 The detection wavelength 304\u003c\/p\u003e \u003cp\u003e19.8 Derivatization 306\u003c\/p\u003e \u003cp\u003e19.9 Unexpected peaks: Ghost and system peaks 308\u003c\/p\u003e \u003cp\u003e\u003cb\u003e20 Quality Assurance 311\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e20.1 Is it worth the effort? 311\u003c\/p\u003e \u003cp\u003e20.2 Verification with a second method 312\u003c\/p\u003e \u003cp\u003e20.3 Method validation 312\u003c\/p\u003e \u003cp\u003e20.4 Standard operating procedures 314\u003c\/p\u003e \u003cp\u003e20.5 Measurement uncertainty 315\u003c\/p\u003e \u003cp\u003e20.6 Qualifications, instrument test and system suitability test 317\u003c\/p\u003e \u003cp\u003e20.7 The quest for quality 318\u003c\/p\u003e \u003cp\u003e\u003cb\u003e21 Preparative HPLC 321\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e21.1 Problem 321\u003c\/p\u003e \u003cp\u003e21.2 Preparative HPLC in practice 322\u003c\/p\u003e \u003cp\u003e21.3 Overloading effects 325\u003c\/p\u003e \u003cp\u003e21.4 Fraction collection 328\u003c\/p\u003e \u003cp\u003e21.5 Recycling 330\u003c\/p\u003e \u003cp\u003e21.6 Displacement chromatography 331\u003c\/p\u003e \u003cp\u003e\u003cb\u003e22 Separation of Enantiomers 333\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e22.1 Introduction 333\u003c\/p\u003e \u003cp\u003e22.2 Chiral mobile phases 335\u003c\/p\u003e \u003cp\u003e22.3 Chiral liquid stationary phases 336\u003c\/p\u003e \u003cp\u003e22.4 Chiral solid stationary phases 337\u003c\/p\u003e \u003cp\u003e22.5 Indirect separation of enantiomers 345\u003c\/p\u003e \u003cp\u003e\u003cb\u003e23 Special Possibilities 349\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e23.1 Micro, capillary and chip HPLC 349\u003c\/p\u003e \u003cp\u003e23.2 High-speed and super-speed HPLC 352\u003c\/p\u003e \u003cp\u003e23.3 Fast separations at 1000 bar: UHPLC 353\u003c\/p\u003e \u003cp\u003e23.4 HPLC with supercritical mobile phases 355\u003c\/p\u003e \u003cp\u003e23.5 HPLC with superheated water 359\u003c\/p\u003e \u003cp\u003e23.6 Electrochromatography 361\u003c\/p\u003e \u003cp\u003e\u003cb\u003e24 Appendix 1: Applied HPLC Theory 363\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e25 Appendix 2: How to Perform the Instrument Test 373\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e25.1 Introduction 373\u003c\/p\u003e \u003cp\u003e25.2 Test sequence 373\u003c\/p\u003e \u003cp\u003e25.3 Preparations 374\u003c\/p\u003e \u003cp\u003e25.4 Pump test. 377\u003c\/p\u003e \u003cp\u003e25.5 UV detector test 379\u003c\/p\u003e \u003cp\u003e25.6 Autosampler test 383\u003c\/p\u003e \u003cp\u003e25.7 Column oven test 383\u003c\/p\u003e \u003cp\u003e25.8 Equations and calculations. 384\u003c\/p\u003e \u003cp\u003e25.9 Documentation 385\u003c\/p\u003e \u003cp\u003e\u003cb\u003e26 Appendix 3: Troubleshooting 387\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e26.1 Pressure problems 387\u003c\/p\u003e \u003cp\u003e26.2 Leak in the pump system 389\u003c\/p\u003e \u003cp\u003e26.3 Deviating retention times 389\u003c\/p\u003e \u003cp\u003e26.4 Injection problems 390\u003c\/p\u003e \u003cp\u003e26.5 Baseline problems 390\u003c\/p\u003e \u003cp\u003e26.6 Peak shape problems 392\u003c\/p\u003e \u003cp\u003e26.7 Problems with light-scattering detectors 393\u003c\/p\u003e \u003cp\u003e26.8 Other causes 394\u003c\/p\u003e \u003cp\u003e26.9 Instrument test 395\u003c\/p\u003e \u003cp\u003e\u003cb\u003e27 Appendix 4: Column Packing 397\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIndex of Separations 401\u003c\/p\u003e \u003cp\u003eSubject Index 403\u003c\/p\u003e","brand":"John Wiley \u0026 Sons Inc","offers":[{"title":"Default Title","offer_id":49402407616855,"sku":"9780470682180","price":123.26,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9780470682180.jpg?v=1730480308","url":"https:\/\/bookcurl.com\/products\/practical-highperformance-liquid-chromatography-9780470682180","provider":"Book Curl","version":"1.0","type":"link"}