{"product_id":"practical-highperformance-liquid-chromatography-9780470682173","title":"Practical HighPerformance Liquid Chromatography","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eThis textbook aims to present the knowledge which is necessary for everyday HPLC measurements to be made, in a detailed and easy-to-follow manner. It contains a wealth of practical material and covers a wide range of subjects.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTrade Review\u003c\/b\u003e\u003cbr\u003e\"This book is very much ‘‘an aid to this end'' and should be number one on every young chromatographer's book list. The publishers too have played their part in producing a book that is pleasing on the eye and a pleasure to read.\" (Chromatographia, 1 December 2010)\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003ePreface to the Fifth Edition.  \u003cp\u003eImportant and Useful Equations for HPLC.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Introduction.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 HPLC: A powerful separation method.\u003c\/p\u003e \u003cp\u003e1.2 A first HPLC experiment.\u003c\/p\u003e \u003cp\u003e1.3 Liquid chromatographic separation modes.\u003c\/p\u003e \u003cp\u003e1.4 The HPLC instrument.\u003c\/p\u003e \u003cp\u003e1.5 Safety in the HPLC laboratory.\u003c\/p\u003e \u003cp\u003e1.6 Comparison between high-performance liquid chromatography and gas chromatography.\u003c\/p\u003e \u003cp\u003e1.7 Comparison between high-performance liquid chromatography and capillary electrophoresis.\u003c\/p\u003e \u003cp\u003e1.8 Units for pressure, length and viscosity.\u003c\/p\u003e \u003cp\u003e1.9 Scientific journals.\u003c\/p\u003e \u003cp\u003e1.10 Recommended books.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Theoretical Principles.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 The chromatographic process.\u003c\/p\u003e \u003cp\u003e2.2 Band broadening.\u003c\/p\u003e \u003cp\u003e2.3 The chromatogram and its purport.\u003c\/p\u003e \u003cp\u003e2.4 Graphical representation of peak pairs with different degree of resolution.\u003c\/p\u003e \u003cp\u003e2.5 Factors affecting resolution.\u003c\/p\u003e \u003cp\u003e2.6 Extra-column volumes (dead volumes).\u003c\/p\u003e \u003cp\u003e2.7 Tailing.\u003c\/p\u003e \u003cp\u003e2.8 Peak capacity and statistical resolution probability.\u003c\/p\u003e \u003cp\u003e2.9 Effects of temperature in HPLC.\u003c\/p\u003e \u003cp\u003e2.10 The limits of HPLC.\u003c\/p\u003e \u003cp\u003e2.11 How to obtain peak capacity.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Pumps.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 General requirements.\u003c\/p\u003e \u003cp\u003e3.2 The short-stroke piston pump.\u003c\/p\u003e \u003cp\u003e3.3 Maintenance and repair.\u003c\/p\u003e \u003cp\u003e3.4 Other pump designs.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Preparation of Equipment up to Sample Injection.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Selection of the mobile phase.\u003c\/p\u003e \u003cp\u003e4.2 Preparation of the mobile phase.\u003c\/p\u003e \u003cp\u003e4.3 Gradient systems.\u003c\/p\u003e \u003cp\u003e4.4 Capillary tubing.\u003c\/p\u003e \u003cp\u003e4.5 Fittings.\u003c\/p\u003e \u003cp\u003e4.6 Sample injectors.\u003c\/p\u003e \u003cp\u003e4.7 Sample solution and sample volume.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Solvent Properties.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Table of organic solvents.\u003c\/p\u003e \u003cp\u003e5.2 Solvent selectivity.\u003c\/p\u003e \u003cp\u003e5.3 Miscibility.\u003c\/p\u003e \u003cp\u003e5.4 Buffers.\u003c\/p\u003e \u003cp\u003e5.5 Shelf life of mobile phases.\u003c\/p\u003e \u003cp\u003e5.6 The mixing cross.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Detectors.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 General.\u003c\/p\u003e \u003cp\u003e6.2 UV detectors.\u003c\/p\u003e \u003cp\u003e6.3 Refractive index detectors.\u003c\/p\u003e \u003cp\u003e6.4 Fluorescence detectors.\u003c\/p\u003e \u003cp\u003e6.5 Electrochemical (amperometric) detectors.\u003c\/p\u003e \u003cp\u003e6.6 Light-scattering detectors.\u003c\/p\u003e \u003cp\u003e6.7 Other detectors.\u003c\/p\u003e \u003cp\u003e6.8 Multiple detection.\u003c\/p\u003e \u003cp\u003e6.9 Indirect detection.\u003c\/p\u003e \u003cp\u003e6.10 Coupling with spectroscopy.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Columns and Stationary Phases.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Columns for HPLC.\u003c\/p\u003e \u003cp\u003e7.2 Precolumns.\u003c\/p\u003e \u003cp\u003e7.3 General properties of stationary phases.\u003c\/p\u003e \u003cp\u003e7.4 Silica.\u003c\/p\u003e \u003cp\u003e7.5 Chemically modified silica.\u003c\/p\u003e \u003cp\u003e7.6 Styrene-divinylbenzene.\u003c\/p\u003e \u003cp\u003e7.7 Some other stationary phases.\u003c\/p\u003e \u003cp\u003e7.8 Column care and regeneration.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 HPLC Column Tests.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Simple tests for HPLC columns.\u003c\/p\u003e \u003cp\u003e8.2 Determination of particle size.\u003c\/p\u003e \u003cp\u003e8.3 Determination of breakthrough time.\u003c\/p\u003e \u003cp\u003e8.4 The test mixture.\u003c\/p\u003e \u003cp\u003e8.5 Dimensionless parameters for HPLC column characterization.\u003c\/p\u003e \u003cp\u003e8.6 The van Deemter equation from reduced parameters and its use in column diagnosis.\u003c\/p\u003e \u003cp\u003e8.7 van Deemter curves and other coherences.\u003c\/p\u003e \u003cp\u003e8.8 Diffusion coefficients.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Adsorption Chromatography: Normal-Phase Chromatography.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 What is adsorption?.\u003c\/p\u003e \u003cp\u003e9.2 The eluotropic series.\u003c\/p\u003e \u003cp\u003e9.3 Selectivity properties of the mobile phase.\u003c\/p\u003e \u003cp\u003e9.4 Choice and optimization of the mobile phase.\u003c\/p\u003e \u003cp\u003e9.5 Applications.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Reversed-Phase Chromatography.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Principle.\u003c\/p\u003e \u003cp\u003e10.2 Mobile phases in reversed-phase chromatography.\u003c\/p\u003e \u003cp\u003e10.3 Solvent selectivity and strength.\u003c\/p\u003e \u003cp\u003e10.4 Stationary phases.\u003c\/p\u003e \u003cp\u003e10.5 Method development in reversed-phase chromatography.\u003c\/p\u003e \u003cp\u003e10.6 Applications.\u003c\/p\u003e \u003cp\u003e10.7 Hydrophobic interaction chromatography.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Chromatography with Chemically Bonded Phases.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction.\u003c\/p\u003e \u003cp\u003e11.2 Properties of some stationary phases.\u003c\/p\u003e \u003cp\u003e11.3 Hydrophilic interaction chromatography.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Ion-Exchange Chromatography.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction.\u003c\/p\u003e \u003cp\u003e12.2 Principle.\u003c\/p\u003e \u003cp\u003e12.3 Properties of ion exchangers.\u003c\/p\u003e \u003cp\u003e12.4 Influence of the mobile phase.\u003c\/p\u003e \u003cp\u003e12.5 Special possibilities of ion exchange.\u003c\/p\u003e \u003cp\u003e12.6 Practical hints.\u003c\/p\u003e \u003cp\u003e12.7 Applications.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Ion-Pair Chromatography.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction.\u003c\/p\u003e \u003cp\u003e13.2 Ion-pair chromatography in practice.\u003c\/p\u003e \u003cp\u003e13.3 Applications.\u003c\/p\u003e \u003cp\u003e13.4 Appendix: UV detection using ion-pair reagents.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Ion Chromatography.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e14.1 Principle.\u003c\/p\u003e \u003cp\u003e14.2 Suppression techniques.\u003c\/p\u003e \u003cp\u003e14.3 Phase systems.\u003c\/p\u003e \u003cp\u003e14.4 Applications.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Size-Exclusion Chromatography.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e15.1 Principle.\u003c\/p\u003e \u003cp\u003e15.2 The calibration chromatogram.\u003c\/p\u003e \u003cp\u003e15.3 Molecular mass determination by means of size-exclusion chromatography.\u003c\/p\u003e \u003cp\u003e15.4 Coupled size-exclusion columns.\u003c\/p\u003e \u003cp\u003e15.5 Phase systems.\u003c\/p\u003e \u003cp\u003e15.6 Applications.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16 Affinity Chromatography.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e16.1 Principle.\u003c\/p\u003e \u003cp\u003e16.2 Affinity chromatography as a special case of HPLC.\u003c\/p\u003e \u003cp\u003e16.3 Applications.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e17 Choice of Method.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e17.1 The various possibilities.\u003c\/p\u003e \u003cp\u003e17.2 Method transfer.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e18 Solving the Elution Problem.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e18.1 The elution problem.\u003c\/p\u003e \u003cp\u003e18.2 Solvent gradients.\u003c\/p\u003e \u003cp\u003e18.3 Column switching.\u003c\/p\u003e \u003cp\u003e18.4 Comprehensive two-dimensional HPLC.\u003c\/p\u003e \u003cp\u003e18.5 Optimization of an isocratic chromatogram using four solvents.\u003c\/p\u003e \u003cp\u003e18.6 Optimization of the other parameters.\u003c\/p\u003e \u003cp\u003e18.7 Mixed stationary phases.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e19 Analytical HPLC.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e19.1 Qualitative analysis.\u003c\/p\u003e \u003cp\u003e19.2 Trace analysis.\u003c\/p\u003e \u003cp\u003e19.3 Quantitative analysis.\u003c\/p\u003e \u003cp\u003e19.4 Recovery.\u003c\/p\u003e \u003cp\u003e19.5 Peak-height and peak-area determination for quantitative analysis.\u003c\/p\u003e \u003cp\u003e19.6 Integration errors.\u003c\/p\u003e \u003cp\u003e19.7 The detection wavelength.\u003c\/p\u003e \u003cp\u003e19.8 Derivatization.\u003c\/p\u003e \u003cp\u003e19.9 Unexpected peaks: Ghost and system peaks.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e20 Quality Assurance.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e20.1 Is it worth the effort?.\u003c\/p\u003e \u003cp\u003e20.2 Verification with a second method.\u003c\/p\u003e \u003cp\u003e20.3 Method validation.\u003c\/p\u003e \u003cp\u003e20.4 Standard operating procedures.\u003c\/p\u003e \u003cp\u003e20.5 Measurement uncertainty.\u003c\/p\u003e \u003cp\u003e20.6 Qualifications, instrument test and system suitability test.\u003c\/p\u003e \u003cp\u003e20.7 The quest for quality.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e21 Preparative HPLC.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e21.1 Problem.\u003c\/p\u003e \u003cp\u003e21.2 Preparative HPLC in practice.\u003c\/p\u003e \u003cp\u003e21.3 Overloading effects.\u003c\/p\u003e \u003cp\u003e21.4 Fraction collection.\u003c\/p\u003e \u003cp\u003e21.5 Recycling.\u003c\/p\u003e \u003cp\u003e21.6 Displacement chromatography.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e22 Separation of Enantiomers.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e22.1 Introduction.\u003c\/p\u003e \u003cp\u003e22.2 Chiral mobile phases.\u003c\/p\u003e \u003cp\u003e22.3 Chiral liquid stationary phases.\u003c\/p\u003e \u003cp\u003e22.4 Chiral solid stationary phases.\u003c\/p\u003e \u003cp\u003e22.5 Indirect separation of enantiomers.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e23 Special Possibilities.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e23.1 Micro, capillary and chip HPLC.\u003c\/p\u003e \u003cp\u003e23.2 High-speed and super-speed HPLC.\u003c\/p\u003e \u003cp\u003e23.3 Fast separations at 1000 bar: UHPLC.\u003c\/p\u003e \u003cp\u003e23.4 HPLC with supercritical mobile phases.\u003c\/p\u003e \u003cp\u003e23.5 HPLC with superheated water.\u003c\/p\u003e \u003cp\u003e23.6 Electrochromatography.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e24 Appendix 1: Applied HPLC Theory.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e25 Appendix 2: How to Perform the Instrument Test.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e25.1 Introduction.\u003c\/p\u003e \u003cp\u003e25.2 Test sequence.\u003c\/p\u003e \u003cp\u003e25.3 Preparations.\u003c\/p\u003e \u003cp\u003e25.4 Pump test.\u003c\/p\u003e \u003cp\u003e25.5 UV detector test.\u003c\/p\u003e \u003cp\u003e25.6 Autosampler test.\u003c\/p\u003e \u003cp\u003e25.7 Column oven test.\u003c\/p\u003e \u003cp\u003e25.8 Equations and calculations.\u003c\/p\u003e \u003cp\u003e25.9 Documentation.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e26 Appendix 3: Troubleshooting.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e26.1 Pressure problems.\u003c\/p\u003e \u003cp\u003e26.2 Leak in the pump system.\u003c\/p\u003e \u003cp\u003e26.3 Deviating retention times.\u003c\/p\u003e \u003cp\u003e26.4 Injection problems.\u003c\/p\u003e \u003cp\u003e26.5 Baseline problems.\u003c\/p\u003e \u003cp\u003e26.6 Peak shape problems.\u003c\/p\u003e \u003cp\u003e26.7 Problems with light-scattering detectors.\u003c\/p\u003e \u003cp\u003e26.8 Other causes.\u003c\/p\u003e \u003cp\u003e26.9 Instrument test.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e27 Appendix 4: Column Packing.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eIndex of Separations.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSubject Index.\u003c\/b\u003e\u003c\/p\u003e","brand":"John Wiley \u0026 Sons Inc","offers":[{"title":"Default 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