{"product_id":"plasmodesmata-9781071621349","title":"Plasmodesmata","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eThis fully updated book aims to facilitate the study of the nanochannels that connect plant cells, known as plasmodesmata, and to instigate new research that will further advance our knowledge of these structures. Beginning with the general structural composition and regulation of plasmodesmata as well as their role in plant development and disease, the volume continues with chapters exploring plasmodesmata architectures and distribution in cell interfaces, approaches to dissect plasmodesmata composition, protocols to quantify changes in plasmodesmata permeability using fluorescent tracers and mobile proteins, as well as a section with protocols that contribute to plasmodesmata research but fall outside the previous classifications. Written for the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trou\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003ePart I: \u003c\/b\u003e\u003cb\u003eOverview Articles\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e1. Plasmodesmata Structural Components and Their Role in Signaling and Plant Development\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Philip Kirk and Yoselin Benitez-Alfonso\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e2. Function of Plasmodesmata in the Interaction of Plants with Microbes and Viruses\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Caiping Huang and Manfred Heinlein\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003ePart II: \u003c\/b\u003e\u003cb\u003eStructure and Distribution of Plasmodesmata\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e3. Plasmodesmata Ultrastructure Determination Using Electron Tomography\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Jules D. Petit, Marie Glavier, Lysiane Brocard, and Emmanuelle Bayer\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e4. Ultrastructural Analysis and Three-Dimensional Reconstruction of Plasmodesmata\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Kamila Godel-Jędrychowska, Tilman Franke, and Ewa Kurczyńska\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e5. Serial Block Electron Microscopy to Study Plasmodesmata in the Vasculature of \u003ci\u003eArabidopsis thaliana\u003c\/i\u003e Roots\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Andrea Paterlini and Ilya Belevich\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e6. Focused Ion Beam-Scanning Electron Microscopy for Investigating Plasmodesmal Densities\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Brandon C. Reagan, John R. Dunlap, and Tessa M. Burch-Smith\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e7. Measuring Plasmodesmata Density on Cell Interfaces of Monocot Leaves Using 3D Immunolocalization and Scanning Electron Microscopy\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Florence R. Danila\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e8. Super-Resolution Imaging of Plasmodesmata Using 3D-Structured Illumination Microscopy\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Kirsten Knox\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003ePart III: \u003c\/b\u003e\u003cb\u003eCompositional Analysis of Plasmodesmata\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e9. In Vivo Aniline Blue Staining and Semi-Automated Quantification of Callose Deposition at Plasmodesmata\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Caiping Huang, Jerôme Mutterer, and Manfred Heinlein\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e10. Immunofluorescence Detection of Callose in Plant Tissue Sections\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Sam Amsbury and Yoselin Benitez-Alfonso\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e11. Callose Detection and Quantification at Plasmodesmata in Bryophytes\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Arthur Muller, Tomomichi Fujita, and Yoan Coudert\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e12. Isolation of Plasmodesmata Membranes for Lipidomic and Proteomic Analysis\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Laetitia Fouillen, Stéphane Claverol, Emmanuelle M.F. Bayer, and Magali S. Grison\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e13. \u003c\/b\u003e\u003cb\u003eMethods for Detection of Protein Interactions with Plasmodesmata-Localized Reticulons\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Verena Kriechbaumer and Stanley W. Botchway\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cb\u003e14. Studying Protein-Protein Interactions at Plasmodesmata by Measuring Förster Resonance Energy Transfer\u003c\/b\u003e\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e            Patrick Blümke, Vicky Howe, and Rüdiger Simon\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e15. Quantifying the Organization and Dynamics of the Plant Plasma-Membrane across Scales Using Light Microscopy\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Joseph F. McKenna\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e16. Using Steady-State Fluorescence Anisotropy to Study Protein Clustering\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Ajeet Chaudhary and Kay Schneitz\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003ePart IV: \u003c\/b\u003e\u003cb\u003eFunctional Analysis of Plasmodesmata\u003c\/b\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e17. Quantification of Cell-to-Cell Connectivity Using Particle Bombardment\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Estee E. Tee, Sebastian Samwald, and Christine Faulkner\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e18. Investigating Plasmodesmata Function in \u003ci\u003eArabidopsis thaliana \u003c\/i\u003eUsing a Low-Pressure Bombardment System and GFP Movement Assay\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Jessica C. Fernandez and Tessa M. Burch-Smith\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e19. Quantifying Plasmodesmatal Transport with an Improved GFP Movement Assay\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Wilson Horner and Jacob O. Brunkard\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e20. \u003c\/b\u003e\u003cb\u003eAn Arabidopsis Callus Grafting Method to Test Cell-to-Cell Mobility of Proteins\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Frank Machin, Yağmur Hasbioğlu, and Friedrich Kragler\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e21. Quantification of Plasmodesmata Permeability in Arabidopsis Leaves by Tracing the Movement of GFP\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Min Diao and Shanjin Huang\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e22. Tracking Intercellular Movement of Fluorescent Proteins in Bryophytes\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Takumi Tomoi, Yoan Coudert, and Tomomichi Fujita\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e23. Virus Genome-Based Reporter for Analyzing Viral Movement Proteins and Plasmodesmata Permeability\u003c\/b\u003e\u003c\/p\u003e              Ekaterina A. Lazareva, Anastasia K. Atabekova, Alexander A. Lezzhov, Sergey Y. Morozov, Manfred Heinlein, and Andrey G. Solovyev\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e24. \u003c\/b\u003e\u003cb\u003eAnalysis of the Distribution of Symplasmic Tracers during Zygotic and Somatic Embryogenesis\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Justyna Wróbel-Marek, Kamila Godel-Jędrychowska, and Ewa Kurczyńska\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e25. Quantifying Intercellular Movement and Protein Stoichiometry for Computational Modeling\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Lisa Van den Broeck, Mariah Gobble, and Rosangela Sozzani\u003c\/p\u003e   \u003cp\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e26. Spatiotemporal Specific Blocking of Plasmodesmata by Callose Induction\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Dawei Yan\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e27. A Forward Genetic Approach to Identify Plasmodesmal Trafficking Regulators Based on Trichome Rescue\u003c\/b\u003e\u003c\/p\u003e              Munenori Kitagawa and David Jackson\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003ePart V: \u003c\/b\u003e\u003cb\u003eOther Approaches Contributing to Plasmodesmata Research\u003c\/b\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e28. In Vivo Visualization of Mobile mRNA Particles in Plants Using BglG\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Eduardo J. Peña and Manfred Heinlein\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e29. Multi-Angle In Vivo Imaging of the \u003ci\u003eArabidopsis thaliana\u003c\/i\u003e Shoot Apical Meristem (SAM)\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Michael Fuchs and Jan U. Lohmann\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e30. More Insights from Ultrastructural and Functional Plasmodesmata Data Using PDinsight\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Eva E. Deinum\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e31. \u003c\/b\u003e\u003cb\u003eMeasuring Intercellular Interface Area in Plant Tissues Using Quantitative 3D Image Analysis\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Gwendolyn V. Davis, Richard S. Smith, and George W. Bassel\u003c\/p\u003e","brand":"Springer-Verlag New York Inc.","offers":[{"title":"Default Title","offer_id":52084487749975,"sku":"9781071621349","price":125.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781071621349.jpg?v=1762206541","url":"https:\/\/bookcurl.com\/products\/plasmodesmata-9781071621349","provider":"Book Curl","version":"1.0","type":"link"}