{"product_id":"plant-virology-9781071618349","title":"Plant Virology","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eThis volume discusses traditional and current techniques that are successfully used to diagnose plant viruses and study molecular plant-virus interactions. The chapters in this book cover topics such as in vivo detection of double-stranded RNA, developing rice mutant using CRISPR-Cas9-based technology, protein-protein interaction assays, purification and transfection of protoplasts, protocols for gene silencing, and transmission electron microscopy. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \u003cbr\u003e\u003cbr\u003eCutting-edge and practical,\u003ci\u003e Plant Virology: Methods and Protocols\u003c\/i\u003e is a valuable resource for plant pathologists, microbiologists, virologists, graduate students, and teachers who are interested in learning more about th\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003ePreface…\u003cbr\u003eTable of Contents…\u003cbr\u003eContributing Authors…\u003cbr\u003e\u003cbr\u003e1\tIn Vivo Detection of Double-Stranded RNA by dRBFC Assay\u003cbr\u003eXiaofei Cheng, Yameng Luan, and Aiming Wang\u003cbr\u003e\u003cbr\u003e2\tDeveloping Rice Mutants using CRISPR\/Cas9-Based Genome Editing Technology\u003cbr\u003eKeyan Xu and Yi Li\u003cbr\u003e\u003cbr\u003e3\tMicroscale Thermophoresis Assay: A Powerful Method to Quantify Protein-Nucleic Acid and Protein-Protein Interactions\u003cbr\u003eYu Huang and Yi Li\u003cbr\u003e\u003cbr\u003e4\tTransient Expression-Mediated Gene Silencing in Plants and Suppression of Gene Silencing with Viral Suppressors \u003cbr\u003eFangfang Li and Aiming Wang\u003cbr\u003e\u003cbr\u003e5\tIsolation and Transfection of Plant Mesophyll Protoplasts for Virology Research \u003cbr\u003eZhaoji Dai and Aiming Wang\u003cbr\u003e\u003cbr\u003e6\tSplit-Luciferase Complementation for Analysis of Virus-Host Protein Interactions\u003cbr\u003eYan Liang and Zhenghe Li\u003cbr\u003e\u003cbr\u003e7\tMonitoring Virus Intercellular Movement from Primary Infected Cells to Neighboring Cells in Plants \u003cbr\u003eZhaoji Dai and Aiming Wang \u003cbr\u003e\u003cbr\u003e8\tSimultaneous Determination and Subcellular Localization of Protein-Protein Interactions in Plant Cells using Bimolecular Fluorescence Complementation Assay \u003cbr\u003eZiwei Tang, Mark A. Bernards, and Aiming Wang\u003cbr\u003e\u003cbr\u003e9\tA Powerful Method for Studying Protein-Protein Interactions in Plants: Co-immunoprecipitation (Co-IP) Assay \u003cbr\u003eZhihao Jiang, Meng Yang, Qing Cao, Yongliang Zhang, and Dawei Li\u003cbr\u003e\u003cbr\u003e10\tMembrane and Nuclear Yeast Two-Hybrid Systems\u003cbr\u003eQian Chen and Taiyun Wei\u003cbr\u003e\u003cbr\u003e11\tTag-Based Pull-Down Assay \u003cbr\u003eShanwu Lyu, Changwei Zhang, Xilin Hou, and Aiming Wang\u003cbr\u003e\u003cbr\u003e12\tPurification of Plasmodesmata-Enriched Fraction for Proteomic Analyses\u003cbr\u003eRongrong He, Mark A. Bernards, and Aiming Wang \u003cbr\u003e\u003cbr\u003e13\tPurification and Proteomics Analysis of Phloem Tissues from Virus-Infected Plants\u003cbr\u003eAaron Simkowich, Susanne E. Kohalmi, and Aiming Wang \u003cbr\u003e\u003cbr\u003e14\tRapid and Specific Purification of Argonaute-Small RNA Complexes from Rice for Slicer Activity \u003cbr\u003eShanshan Zhao and Jianguo Wu\u003cbr\u003e\u003cbr\u003e15\tSilencing Specific Genes in Plants Using Virus-Induced Gene Silencing (VIGS) Vectors \u003cbr\u003eTao Zhou, Laihua Dong, Tong Jiang, and Zaifeng Fan \u003cbr\u003e\u003cbr\u003e16\tA Versatile Expression Platform in Insects and Cereals Based on a Cytorhabdovirus\u003cbr\u003eJi-Hui Qiao, Qiang Gao, Ying Zang, Xiao-Dong Fang, and Xian-Bing Wang \u003cbr\u003e\u003cbr\u003e17\tProduction of Virus-Free Chrysanthemum morifolium Ramat by Tissue Culture Techniques\u003cbr\u003eKeru Yan, Xuejie Du, and Bizeng Mao\u003cbr\u003e\u003cbr\u003e18\tLong-Term Preservation of Plant Viruses in Cryopreserved Shoot Tips\u003cbr\u003eMin-Rui Wang, Tianxing Pang, Ziqian Lian, Qiao-Chun Wang, and Liying Sun \u003cbr\u003e\u003cbr\u003e19\tEstablishment of White-Backed Planthopper Cell Lines \u003cbr\u003eHongyan Chen, Wei Wu, and Taiyun Wei\u003cbr\u003e\u003cbr\u003e20\tBiolistic Inoculation of Fruit Tress with Full-Length Infectious cDNA Clones of RNA Viruses \u003cbr\u003eYinzi Li and Aiming Wang\u003cbr\u003e\u003cbr\u003e21\tPurification of Total RNAs and Small RNAs from Fruit Tree Leaf Tissues\u003cbr\u003eYuxin Ma and Shifang Li\u003cbr\u003e\u003cbr\u003e22\tDiagnosis of Viral Diseases Using Deep Sequencing and Metagenomics Analyses\u003cbr\u003eAli Raza and Qingfa Wu\u003cbr\u003e\u003cbr\u003e23\tImmunocapture-Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay for Detection of Plant RNA Viruses\u003cbr\u003eSubhas Hajeri and Raymond Yokomi\u003cbr\u003e\u003cbr\u003e24\tDetection of Tomato spotted wilt virus (TSWV) Infection in Plants using DAS-ELISA and Dot-ELISA \u003cbr\u003eShibo Gao and Jianxiang Wu \u003cbr\u003e\u003cbr\u003e25\tTube Capture (TC) RT-PCR and Multiplex RT-PCR for Diagnosis and Characterization of Viruses in Fruit Trees \u003cbr\u003eLiping Wang\u003cbr\u003e\u003cbr\u003e26\tDetection of Cucumber green mottle mosaic virus (CGMMV) in Cucurbitaceous Crop Seeds by RT-PCR\u003cbr\u003eYi Xie and Jianxiang Wu\u003cbr\u003e\u003cbr\u003e27\tRNA In Situ Hybridization of Detecting Cucumber Mosaic Virus in Shoots of Nicotiana benthamiana Plants \u003cbr\u003eJing-Ze Zou, De-Shui Liu, Xin Tong, Xiao-Peng Zhang, and Xian-Bing Wang\u003cbr\u003e\u003cbr\u003e28\tTransmission Electron Microscopic Methods for Plant Virology \u003cbr\u003eLi Xie and Jian Hong \u003cbr\u003e\u003cbr\u003eSubject Index List…\u003c\/p\u003e","brand":"Springer-Verlag New York Inc.","offers":[{"title":"Default Title","offer_id":53186413461847,"sku":"9781071618349","price":179.99,"currency_code":"GBP","in_stock":true}],"url":"https:\/\/bookcurl.com\/products\/plant-virology-9781071618349","provider":"Book Curl","version":"1.0","type":"link"}