{"product_id":"plant-genome-engineering-9781071631300","title":"Plant Genome Engineering","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eThis volume provides readers with a collection of the latest protocols used to study plant genome editing and trait engineering. The chapters in this book are organized into five parts: genome engineering systems; machinery design and validation; delivery tools; generation and analysis of engineering materials; and crop genome engineering applications. The chapters cover topics such as methods of applying the popular CRISPR-Cas9 or CRISPR-Cas12 systems for editing genomes in different crop species, the use of small synthetic plastome for potato genome engineering, and the use of CRISPR-Cas9 for algal cell genome engineering. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e\u003cbr\u003eCutting-edge and comprehensive, \u003ci\u003ePlant Genome Engin\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003c\/i\u003e\u003c\/p\u003e\u003cp\u003ePreface…\u003cbr\u003eTable of Contents…\u003cbr\u003eContributing Authors…\u003cbr\u003e\u003cbr\u003ePART I GENOME ENGINEERING SYSTEMS \u003cbr\u003e\u003cbr\u003e1\tPAM-Less CRISPR-SpRY Genome Editing in Plants\u003cbr\u003eSimon Sretenovic, Xu Tang, Qiurong Ren, Yong Zhang, and Yiping Qi\u003cbr\u003e\u003cbr\u003e2 \tType I-D CRISPR System-Mediated Genome Editing in Plants\u003cbr\u003eNaoki Wada, Keishi Osakabe, and Yuriko Osakabe\u003cbr\u003e\u003cbr\u003e3\tCRISPR\/LbCas12a-Mediated Genome Editing in Soybean\u003cbr\u003eDawei Liang, Yubo Liu, Chao Li, Qin Wen, Jianping Xu, Lizhao Geng, Chunxia Liu, Huaibing Jin, Yang Gao, Heng Zhong, John Dawson, Bin Tian, Brenden Barco, Xiujuan Su, Shujie Dong, Changbao Li, Sivamani Elumalai, Qiudeng Que, Ian Jepson, and Liang Shi\u003cbr\u003e\u003cbr\u003e4\tBase Editing in Poplar through an Agrobacterium-Mediated Transformation Method\u003cbr\u003eGen Li, Simon Sretenovic, Gary Coleman, and Yiping Qi\u003cbr\u003e\u003cbr\u003e5\tGenetic Engineering of Potato (Solanum tuberosum) Chloroplasts using the Small Synthetic Plastome “Mini-Synplastome”\u003cbr\u003eAlessandro Occhialini, Alexander C. Pfotenhauer, Henry Daniell, C. Neal Stewart, Jr, and Scott C. Lenaghan\u003cbr\u003e\u003cbr\u003ePART II DESIGN AND VALIDATION TOOLS\u003cbr\u003e\u003cbr\u003e6\tDesigning Guide-RNA for Generating Premature Stop Codons for Gene Knockout using CRISPR-BETS\u003cbr\u003eYuechao Wu and Tao Zhang\u003cbr\u003e\u003cbr\u003e7\tConstruction of CRISPR\/Cas9 Multiplex Genome Editing System in Rice\u003cbr\u003eJie Xiong, Chun Wang, and Kejian Wang\u003cbr\u003e\u003cbr\u003e8\tUse of Fluorescent Protein Reporters for Assessing and Detecting Genome Editing Reagents and Transgene Expression in Plants\u003cbr\u003eGuoliang Yuan, Gerald A. Tuskan, and Xiaohan Yang\u003cbr\u003e\u003cbr\u003e9\tAutomated, High-Throughput Protoplast Transfection for Gene Editing and Transgene Expression Studies\u003cbr\u003eStephen B. Rigoulot, Brenden Barco, Yingxiao Zhang, Chengjin Zhang, Kerry A. Meier, Matthew Moore, Jonathan Fabish, Rachel Whinna, Jeongmoo Park, Erin M. Seaberry, Aditya Gopalan, Shujie Dong, Zhongying Chen, and Qiudeng Que\u003cbr\u003e\u003cbr\u003ePART III TRANSGENE-FREE DELIVERY OF MACHINERY\u003cbr\u003e\u003cbr\u003e10\tDelivery of CRISPR-Cas12a Ribonucleoprotein Complex for Genome Editing in an Embryogenic Citrus Cell Line\u003cbr\u003eHong Fang, James N. Culver, Randall P. Niedz, and Yiping Qi\u003cbr\u003e\u003cbr\u003e11\tTransgene-Free Genome Editing in Nicotiana benthamiana with CRISPR\/Cas9 Delivered by a Rhabdovirus Vector \u003cbr\u003eXiaonan Ma, Xuemei Li, and Zhenghe Li\u003cbr\u003e\u003cbr\u003e12\tRibonucleoprotein (RNP)-Mediated Targeted Mutagenesis in Barley (Hordeum vulgare L.)\u003cbr\u003eMartin Becker and Goetz Hensel\u003cbr\u003e\u003cbr\u003e13\tRibonucleoprotein (RNP)-Mediated Allele Replacement in Barley (Hordeum vulgare L.) Leaves\u003cbr\u003eMartin Becker and Goetz Hensel\u003cbr\u003e\u003cbr\u003e14\tGenome Editing in Chlamydomonas reinhardtii Using Cas9-gRNA Ribonucleoprotein Complex: A Step-by-Step Guide\u003cbr\u003eDhananjay Dhokane, Nagesh Kancharla, Arockiasamy Savarimuthu, Bhaskar Bhadra, Anindya Bandyopadhyay, and Santanu Dasgupta\u003cbr\u003e\u003cbr\u003ePART IV GENERATION AND ANALYSIS OF ENGINEERED LINES\u003cbr\u003e\u003cbr\u003e15 \tHighly Efficient Gene Knockout in Medicago truncatula Genotype R108 using CRISPR-Cas9 System and an Optimized Agrobacterium Transformation Method\u003cbr\u003eTom Lawrenson, Nicola Atkinson, Macarena Forner, and Wendy Harwood\u003cbr\u003e\u003cbr\u003e16\tEfficient Targeted Mutagenesis in Brassica Crops using CRISPR\/Cas Systems\u003cbr\u003eTom Lawrenson, Mark Youles, Monika Chhetry, Martha Clarke, Wendy Harwood, and Penny Hundleby\u003cbr\u003e\u003cbr\u003e17\tIntroduction of Genome Editing Reagents and Genotyping of Derived Edited Alleles in Soybean (Glycine max (L.) Merr.)\u003cbr\u003eTruyen Quach, Hanh Nguyen, Olivia Meyer, Shirley J. Sato, Tom Elmo Clemente, and Ming Guo\u003cbr\u003e\u003cbr\u003e18\tA CRISPR\/Cas9 Protocol for Target Gene Editing in Barley\u003cbr\u003eQiantao Jiang, Qiang Yang, Wendy Harwood, Huaping Tang, Yuming Wei, and Youliang Zheng\u003cbr\u003e\u003cbr\u003e19\tTargeted Insertion in Nicotiana benthamiana Genomes via Protoplast Regeneration\u003cbr\u003eFu-Hui Wu, Chen-Tran Hsu, and Choun-Sea Lin\u003cbr\u003e\u003cbr\u003e20\tStepwise Optimization of Real-Time RT-PCR Analysis \u003cbr\u003eNathan A. Maren, James R. Duduit, Debao Huang, Fanghou Zhao, Thomas G. Ranney, and Wusheng Liu\u003cbr\u003e\u003cbr\u003ePART V GENOME ENGINEERING FOR CROP IMPROVEMENT \u003cbr\u003e\u003cbr\u003e21\tCRISPR\/Cas9 Technology for Potato Functional Genomics and Breeding\u003cbr\u003eMatías Nicolás González, Gabriela Alejandra Massa, Mariette Andersson, Leonardo Storani, Niklas Olsson, Cecilia Andrea Décima Oneto, Per Hofvander, and Sergio Enrique Feingold\u003cbr\u003e\u003cbr\u003e22\tRecent Advances in Engineering of In Vivo Haploid Induction Systems\u003cbr\u003eJian Lv and Timothy Kelliher\u003cbr\u003e\u003cbr\u003eSubject Index List…\u003c\/p\u003e\u003cbr\u003e","brand":"Springer-Verlag New York Inc.","offers":[{"title":"Default Title","offer_id":51039241011543,"sku":"9781071631300","price":999.99,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781071631300.jpg?v=1750943025","url":"https:\/\/bookcurl.com\/products\/plant-genome-engineering-9781071631300","provider":"Book Curl","version":"1.0","type":"link"}