{"product_id":"pirna-9781071623794","title":"piRNA","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eThis detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several bioinformatic and biophysical methods related to piRNA studies, consistent with the increasing importance of high-throughput sequencing and computational methods. Written for the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. \u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\u003cdiv\u003eAuthoritative and up-to-date, \u003ci\u003epiRNA: Methods and Protocols\u003c\/i\u003e serves as an ideal guide for researchers seeking to elucidate the numerous mysteries of this area of multicellular biology.\u003c\/div\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003ePart I: Methods to Study the Nature and Function of piRNAs in Non-Classical Organisms\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e1. Functional Analysis of Individual piRNAs in \u003ci\u003eAedes aegypti \u003c\/i\u003eCells and\u003ci\u003e \u003c\/i\u003eEmbryos\u003ci\u003e \u003c\/i\u003eUsing Antisense Oligonucleotides\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Rebecca Halbach and Pascal Miesen\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e2. CRISPR-Mediated Genome Engineering in\u003ci\u003e Aedes aegypti\u003c\/i\u003e\u003c\/b\u003e\u003c\/p\u003e              Ruichen Sun, Ming Li, Conor J. McMeniman, and Omar S. Akbari\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e3. PIWI-Directed DNA Elimination for Tetrahymena Genetics\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Salman Shehzada and Kazufumi Mochizuki\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e4. Planarian PIWI-piRNA Interaction Analysis Using Immunoprecipitation and piRNA-Sequencing\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Makoto Kashima, Atsumi Miyata, and Norito Shibata\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e5. Isolation and Processing of Bovine Oocytes for Small RNA Sequencing\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Minjie Tan, Helena T.A. van Tol, and Elke F. Roovers\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e6. 3D Imaging and In Situ Hybridization for Uncovering the Functions of MicroRNA in Rice Anther\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Koji Koizumi and Reina Komiya\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003ePart II: Methods to Study Roles of piRNAs in Classic Model Organisms\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e7. Cloning, Sequencing, and Linkage Analysis of piRNAs\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Rippei Hayashi\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e8. Drosophila Genetic Resources for Elucidating piRNA Pathway\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Kuniaki Saito\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e9. Generation of Stable Drosophila Ovarian Somatic Cell Lines Using the \u003ci\u003epiggyBac\u003c\/i\u003e System\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Chikara Takeuchi, Kensaku Murano, Mitsuru Ishikawa, Hideyuki Okano, and Yuka W. Iwasaki\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003ePart III: Methods to Study Nuclear Regulation by Other Non-Coding RNAs\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e10. Whole Mount RNA FISH Combined with Immunofluorescence for Analysis of the Telomeric Ribonucleoproteins in the Drosophila\u003ci\u003e \u003c\/i\u003eGermline\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Valeriya Morgunova, Maria M. Sukhova, and Alla Kalmykova\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e11. CRISPR-Mediated Activation of Transposable Elements in Embryonic Stem Cells\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Akihiko Sakashita, Masaru Ariura, and Satoshi H. Namekawa\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e12. Method for Evaluating Effects of Non-Coding RNAs on Nucleosome Stability\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Mariko Dacher, Risa Fujita, Tomoya Kujirai, and Hitoshi Kurumizaka\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e13. Revisiting the Glass Treatment for Single Molecule Analysis of ncRNA Function\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Shuting Shen, Masahiro Naganuma, Yukihide Tomari, and Hisashi Tadakuma\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e14. Low Input Genome-Wide DNA Methylation Analysis with Minimal Library Amplification\u003c\/b\u003e\u003c\/p\u003e              Wan Kin Au Yeung and Hiroyuki Sasaki\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e15. Solid-Support Directional (SSD) RNA-Seq as a Companion Method to CLIP-Seq\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Abd-El Monsif Shawky, Mahmoud Dondeti, Zissimos Mourelatos, and Anastasios Vourekas\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e16. UPA-Seq-Based Search Method for Functional lncRNA Candidates\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Saori Yokoi and Shinichi Nakagawa\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e17. Large-Scale Analysis of RNA-Protein Interactions for Functional RNA Motif Discovery Using FOREST\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Emi Miyashita, Kaoru R. Komatsu, and Hirohide Saito\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003ePart IV: Bioinformatic and Biophysical Methods to Study Non-Coding RNAs\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e18. Computational Methods for the Discovery and Annotation of Viral Integrations\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Umberto Palatini, Elisa Pischedda, and Mariangela Bonizzoni\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e19. Bioinformatics Approaches for Determining the Functional Impact of Repetitive Elements on Non-Coding RNAs\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Chao Zeng, Atsushi Takeda, Kotaro Sekine, Naoki Osato, Tsukasa Fukunaga, and Michiaki Hamada\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e20. Extending and Running the Mosquito Small RNA Genomics Resource Pipeline\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Gargi Dayama, Katia Bulekova, and Nelson C. Lau\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e21. Preparation of Non-Overlapping Transposable Elements (TE) Annotation by Interval Tree\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Shohei Kojima\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e22. Statistical Thermodynamics Approach for Intracellular Phase Separation\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Tomohiro Yamazaki and Tetsuya Yamamoto\u003c\/p\u003e","brand":"Springer-Verlag New York Inc.","offers":[{"title":"Default Title","offer_id":53186413855063,"sku":"9781071623794","price":179.99,"currency_code":"GBP","in_stock":true}],"url":"https:\/\/bookcurl.com\/products\/pirna-9781071623794","provider":"Book Curl","version":"1.0","type":"link"}