{"product_id":"photoswitching-proteins-9781071607572","title":"Photoswitching Proteins","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eThis volume provides cutting edge protocols for using optogenetic and opto-chemical techniques across a wide spectrum of applications. Chapters guide readers through methods in neuro-optogenetics, including methods to dissect local and long-range connectivity ex vivo, investigate G-protein coupled receptors, study astrocyte-neuron networks in vitro and in vivo, employ upconversion nanoparticles for transcranial optogenetic stimulation, and use Adeno-associated virus (AAV) for delivery of optogenetic constructs. At the same time, this book covers a range of methods for non-neuronal optogenetics, including various applications of light-oxygen-voltage domains or cryptochromes for control of nucleocytoplasmic transport, transgene expression and chromatin recruitment. The book further presents protocols for traceless light-control of gene expression with melanopsin, light-inducible cAMP production, use of light-regulated receptor tyrosine kinases (Opto-RTKs), synthesis of light-dependent bi\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e1. Studying Neuronal Function Ex Vivo using Optogenetic Stimulation and Patch Clamp\u003c\/p\u003e  \u003cp\u003eAyla Aksoy-Aksel, Julien Genty, Martin Zeller, and Ingrid Ehrlich\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e2. Optogenetic Techniques for Probing G Protein-Coupled Receptor Signaling\u003c\/p\u003e  \u003cp\u003eNohely Abreu\u003csup\u003e \u003c\/sup\u003eand Joshua Levitz\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e3. Melanopsin for Time-Controlling Activation of Astrocyte-Neuron Networks\u003c\/p\u003e  \u003cp\u003eSara Mederos, Candela González-Arias, and Gertrudis Perea\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e4. Near-infrared Deep Brain Stimulation in Living Mice\u003c\/p\u003e  \u003cp\u003eShuo Chen\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e5. Lab-scale Production of Recombinant Adeno-associated Viruses (AAV) for Expression of Optogenetic Elements \u003c\/p\u003e  \u003cp\u003eJanina Haar, Chiara Krämer, and Dirk Grimm\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e6. AAV-mediated Gene Delivery to Foveal Cones\u003c\/p\u003e  \u003cp\u003eStéphane Bertin, Elena Brazhnikova, Céline Jaillard, José-Alain Sahel, and Deniz Dalkara\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e\u003csup\u003e \u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e7. Engineering Optogenetic Protein Analogs\u003c\/p\u003e  \u003cp\u003eBei Liu, Daniel J. Marston, and Klaus M. Hahn\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  8. Optogenetic Control of Nucleocytoplasmic Protein Transport\u003cp\u003e\u003c\/p\u003e  \u003cp\u003eDaniel Weis and Barbara Di Ventura\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e\u003csup\u003e \u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e9. CRISPR Labeling Light-inducible\u003c\/p\u003e  Mareike D. Hoffmann, Felix Bubeck, and Dominik Niopek\u003csup\u003e\u003c\/sup\u003e\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e10. Optogenetic Control of Gene Expression using Cryptochrome 2 and a Light-activated Degron\u003c\/p\u003e  \u003cp\u003eCarmen N. Hernández-Candia and Chandra L. Tucker\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e11. Optogenetic Downregulation of Protein Levels to Control Programmed Cell Death in Mammalian Cells with a Dual Blue-Light Switch\u003c\/p\u003e  \u003cp\u003ePatrick Fischbach, Patrick Gonschorek, Julia Baaske, Jamie A. Davies, Wilfried Weber, and Matias D. Zurbriggen\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  12. Tracing Reversible Light-induced Chromatin Binding with Near-infrared Fluorescent Proteins \u003cp\u003eAnne Rademacher, Fabian Erdel, Jorge Trojanowski, and Karsten Rippe\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  13. Construction of a Multiwell Light-induction Platform for Traceless Control of Gene Expression in Mammalian Cells\u003cp\u003e\u003c\/p\u003e  \u003cp\u003eMaysam Mansouri, Samson Lichtenstein, Tobias Strittmatter, Peter Buchmann, and Martin Fussenegger\u003c\/p\u003e  \u003cp\u003e\u003csup\u003e \u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e14. Dual-activation of cAMP Production through Photo-Stimulation or Chemical Stimulation\u003c\/p\u003e  \u003cp\u003eNyla Naim, Jeff M. Reece, Xuefeng Zhang, and Daniel L. Altschuler\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e\u003csup\u003e \u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e15. Synthesis of a Light-controlled Phytochrome-based Extracellular Matrix with Reversibly Adjustable Mechanical Properties\u003c\/p\u003e  \u003cp\u003eMaximilian Hörner, Philipp Hoess, Ramona Emig, Balder Rebmann, and Wilfried Weber\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e16. Design and Application of Light-regulated Receptor Tyrosine Kinases\u003c\/p\u003e  \u003cp\u003eStephanie Kainrath and Harald Janovjak\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e17. All-optical Miniaturized Co-culture Assay of Voltage-gated Ca\u003csup\u003e2+\u003c\/sup\u003e Channels\u003c\/p\u003e  \u003cp\u003eViviana Agus and Harald Janovjak\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e18. Optogenetics and CRISPR: A New Relationship Built to Last\u003c\/p\u003e  \u003cp\u003eJan Mathony, Mareike Daniela Hoffmann, and Dominik Niopek\u003c\/p\u003e","brand":"Springer-Verlag New York Inc.","offers":[{"title":"Default Title","offer_id":52195253879127,"sku":"9781071607572","price":999.99,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781071607572.jpg?v=1763646911","url":"https:\/\/bookcurl.com\/products\/photoswitching-proteins-9781071607572","provider":"Book Curl","version":"1.0","type":"link"}