{"product_id":"mycobacteria-protocols-9781071614594","title":"Mycobacteria Protocols","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eThis fully updated edition explores the latest techniques to study the challenging, and at times dangerous, genus of bacteria known as mycobacteria with basic methods that are still required for mycobacteriology along with the newer or improved methods that have been developed. The volume features chapters on the basics of DNA isolation, protein isolation, and lipid isolation, as well as more sophisticated techniques for isolation of ribosomes, and continues with sections involving analyzing subcellular fractions, culture methods, sequencing technology, \u003ci\u003ein vitro\u003c\/i\u003e models, molecular methods, as well as drug discovery applications. Written for the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\u003cdiv\u003eAuthoritative and up-to-date, \u003ci\u003eM\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003c\/i\u003e\u003cp\u003e\u003cb\u003e1. Culturing Mycobacteria\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Elizabeth Wallace, Debra Hendrickson, Nicholas Tolli, Carolina Mehaffy, María Peña, Jerry A. Nick, Phillip Knabenbaur, Jackson Watkins, Anne Simpson, Anita G. Amin, Delphi Chatterjee, Karen M. Dobos, Ramanuj Lahiri, Linda Adams, Michael Strong; Max Salfinger, Rebecca Bradford, Timothy T. Stedman, Marco A. Riojas, and Manzour Hernando Hazbón\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e2. DNA Isolation from Mycobacteria\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Heena Jagatia and Daire Cantillon\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e3. Extraction and Separation of Mycobacterial Proteins\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Megan Lucas, Joan M. Ryan, Jackson Watkins, Kala Early, Nicole A. Kruh-Garcia, Carolina Mehaffy, and Karen M. Dobos\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e4. Lipid and Lipoarabinomannan Isolation and Characterization\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Marie-Antoinette Lanéelle, Lucie Spina, Jérôme Nigou, Anne Lemassu, and Mamadou Daffé\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e5. Purification of Hibernating and Active C- Ribosomes from Zinc-Starved Mycobacteria\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Yunlong Li, Pooja Keshavan, Jamie H. Corro, Ravi K. Koripella, Rajendra K. Agrawal, and Anil K. Ojha\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e6. Macrophage Infection Models for \u003ci\u003eMycobacterium tuberculosis\u003c\/i\u003e\u003c\/b\u003e\u003c\/p\u003e              Benjamin K. Johnson, Sean M. Thomas, Andrew J. Olive, and Robert B. Abramovitch\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e7. Novel Single-Cell and High Throughput Microscopy Techniques to Monitor \u003ci\u003eDictyostelium discoideum\u003c\/i\u003e-\u003ci\u003eMycobacterium marinum \u003c\/i\u003eInfection Dynamics\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Manon Mottet, Cristina Bosmani, Nabil Hanna, Jahn Nitschke, Louise Lefrancois, and Thierry Soldati\u003c\/p\u003e   \u003cp\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e8. Single-Cell Analysis of Mycobacteria Using Microfluidics and Time-Lapse Microscopy\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Giulia Manina and Neeraj Dhar\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e9. Measuring Efflux and Permeability in Mycobacteria\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Liliana Rodrigues, José A. Aínsa, and Miguel Viveiros\u003c\/p\u003e   \u003cp\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e10. Drug Susceptibility Screening Using In Vitro Models of Hypoxic Non-Replicating Persistent Mycobacteria\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Savannah E.R. Gibson, James Harrison, and Jonathan A.G. Cox\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e11. Flow Cytometry Analysis of Mycobacteria and Mycobacteria-Infected Immune Cells\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Sydney L. Solomon and Bryan D. Bryson\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e12. \u003c\/b\u003e\u003cb\u003eElectroporation of Mycobacteria\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Tanya Parish\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e13. \u003c\/b\u003e\u003cb\u003eGene Switching and Essentiality Testing\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Amanda Claire Brown\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e14. Oligo-Mediated Recombineering and Its Use for Making SNPs, Knockouts, Insertions, and Fusions in \u003ci\u003eMycobacterium tuberculosis\u003c\/i\u003e\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Kenan C. Murphy\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e15. Using Proteolytic Hypomorphs to Detect Small Molecule Mechanism of Action\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Eachan O. Johnson and Deborah T. Hung\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e16. CRISPR Interference (CRISPRi) for Targeted Gene Silencing in Mycobacteria\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Andrew I. Wong and Jeremy M. Rock\u003c\/p\u003e   \u003cp\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e17. Exploiting Fluorescent Proteins to Understand \u003ci\u003eMycobacterium tuberculosis \u003c\/i\u003eBiology\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            David Giacalone, Lu Huang, and Shumin Tan\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e18. Metabolic Labeling of Live Mycobacteria with Trehalose-Based Probes\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Nicholas Banahene and Benjamin M. Swarts\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e19. Identification and Characterization of Mycobacterial Species Using Whole Genome Sequences\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Marco A. Riojas, Andrew M. Frank, Samuel R. Greenfield, Stephen P. King, Conor J. Meehan, Michael Strong, Alice R. Wattam, and Manzour Hernando Hazbón\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e20. Whole Genome Sequencing of \u003ci\u003eMycobacterium tuberculosis\u003c\/i\u003e Directly from Sputum Samples\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Amanda Claire Brown\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e21. Experimental and Computational Workflow for RNA Sequencing in \u003ci\u003eMycobacterium tuberculosis\u003c\/i\u003e: From Total RNA to Differentially Expressed Genes\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Shuyi Ma, Richard M. Jones Jr., Natalie S. Gleason, Jessica Farrow-Johnson, and David R. Sherman\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e22. RNA Sequencing for Transcript 5′-End Mapping in Mycobacteria\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            M. Carla Martini, Huaming Sun, and Scarlet S. Shell\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e23. Methods for Proteomic Analyses of Mycobacteria\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Carolina Mehaffy, Megan Lucas, Nicole A. Kruh-Garcia, and Karen M. Dobos\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e24. \u003c\/b\u003e\u003cb\u003eTargeted Lipidomics of Mycobacterial Lipids and Glycolipids\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Emilie Layre\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e25. Metabolomics of \u003ci\u003eMycobacterium tuberculosis\u003c\/i\u003e\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Kyle A. Planck, Madhumitha Nandakumar, Luiz Pedro S. de Carvalho, and Kyu Rhee\u003c\/p\u003e   \u003cp\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e26. Determining Minimum Inhibitory Concentrations in Liquid Cultures or on Solid Medium\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Qinglan Wang and Helena I.M. Boshoff\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e27. A Multi-Stress Model for High Throughput Screening Against Non-Replicating \u003ci\u003eMycobacterium tuberculosis\u003c\/i\u003e\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Ben Gold, Thulasi Warrier, and Carl Nathan\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e28. \u003c\/b\u003e\u003cb\u003eAntimicrobial Susceptibility Testing for \u003ci\u003eMycobacterium sps\u003c\/i\u003e in High Throughput Format\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Esther Pérez-Herrán and Alfonso Mendoza\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e29. High-Content Analysis Monitoring Intracellular Trafficking and Replication of \u003ci\u003eMycobacterium tuberculosis\u003c\/i\u003e Inside Host Cells\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Nathalie Deboosere, Imène Belhaouane, Arnaud Machelart, Eik Hoffmann, Alexandre Vandeputte, and Priscille Brodin\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e30. Efficient Measurement of Drug Interactions with DiaMOND (Diagonal Measurement of N-Way Drug Interactions)\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Nhi Van, Yonatan Degefu, and Bree B. Aldridge\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e31. Drug Sensitivity Testing of \u003ci\u003eMycobacterium tuberculosis\u003c\/i\u003e Growing in a Hollow Fiber Bioreactor\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Brett Mason and Véronique Dartois\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Springer-Verlag New York Inc.","offers":[{"title":"Default Title","offer_id":51039240290647,"sku":"9781071614594","price":251.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781071614594.jpg?v=1750943027","url":"https:\/\/bookcurl.com\/products\/mycobacteria-protocols-9781071614594","provider":"Book Curl","version":"1.0","type":"link"}