{"product_id":"mrna-decay-9781071641750","title":"mRNA Decay","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eMinimal Perturbation Analysis of mRNA Degradation Rates with Tet Off and RT qPCR.- Northern Blotting: Protocols for Radioactive and Non-Radioactive Detection of RNA.- RNA Blotting by Electrotransfer and RNA Detection.- Dynamic Evolution of Poly-A Tail Lengths Visualized by RNAse H Assay and Northern Blot Using Non-Radioactive Probes in Yeast.- Assessing Deadenylation Activity by Polyacrylamide Gel Electrophoresis Using a Fluorescent RNA Substrate.- Studying Exoribonuclease Activity Using Fluorescence Anisotropy Assay.- Purification of Enzymatically Active Xrn1 for Removal of Non-Capped mRNAs from In Vitro Transcription Reactions and Evaluation of mRNA Decapping Status In Vivo.- Production of Fully Capped mRNA for Transfection into Mammalian Cells: A Protocol for Enzymatic Degradation of Uncapped Transcripts after In Vitro mRNA Synthesis.- Transcriptome-Wide Analysis of the 5' Cap Status of RNA Using 5Monophosphate-Dependent Exonuclease Digestion and RNA Sequencing.- RNA Decay Assay:\u003c\/p\u003e","brand":"Humana","offers":[{"title":"Default Title","offer_id":52090796605783,"sku":"9781071641750","price":189.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781071641750.jpg?v=1762273490","url":"https:\/\/bookcurl.com\/products\/mrna-decay-9781071641750","provider":"Book Curl","version":"1.0","type":"link"}