{"product_id":"methods-in-molecular-biology-protein-chemistry-cloning-and-characterization-of-an-enterotoxin-subunit-9780470843604","title":"Methods in Molecular Biology  Protein Chemistry","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eThe advances made in molecular biology have allowed scientists to manipulate DNA. This means that molecular biology has become a tool for answering scientific questions that may be quite unrelated to genetics and cell biology. The techniques have become a means to an end, rather than an end in themselves. This work deals with this area.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003ePreface\u003cbr\u003e \u003cbr\u003e Introduction and Background\u003cbr\u003e \u003cbr\u003e Part 1 Molecular Biology\u003cbr\u003e \u003cbr\u003e Experiment 1 Instructions for Pipetman Use\u003cbr\u003e \u003cbr\u003e Experiment 2 Preparation of Plasmid Template DNA\u003cbr\u003e \u003cbr\u003e Experiment 3 Estimation of Plasmid DNA Concentration\u003cbr\u003e PCR Amplification of LTB Gene from Plasmid DNA\u003cbr\u003e \u003cbr\u003e Experiment 4 Agarose Gel Eletrophoresis\u003cbr\u003e Recovery of PCR Product\u003cbr\u003e \u003cbr\u003e Experiment 5 Restriction Digest of LTB Insert\u003cbr\u003e Plate Preparation\u003cbr\u003e \u003cbr\u003e Experiment 6 Purification of Digested Insert and Vector by Agarose Gel Eletrophoresis\u003cbr\u003e Recovery of Digested Insert and Vector\u003cbr\u003e \u003cbr\u003e Experiment 7 Determination of DNA Concentrations\u003cbr\u003e Ligation of Insert into Vector\u003cbr\u003e \u003cbr\u003e Experiment 8 Transformation of Host Cells by Construct\u003cbr\u003e \u003cbr\u003e Experiment 9 Colony-pick PCR to Check for Presence of Insert\u003cbr\u003e Streak Plates with Potential Constructs\u003cbr\u003e Inoculate Cultures\/Run PCR\u003cbr\u003e \u003cbr\u003e Experiment 10 Agarose Gel Electrophoresis to Verify Presence of Insert\u003cbr\u003e Purification of the Plasmid Construct using a Plasmid Miniprep Kit\u003cbr\u003e \u003cbr\u003e Experiment 11 DNA Concentration Determination\u003cbr\u003e Ethanol Precipitation of Plasmid DNA\u003cbr\u003e Sequencing Reaction\u003cbr\u003e \u003cbr\u003e Experiment 12 Purification of Extension Reaction Products\u003cbr\u003e Sequencing Gel Demonstration\u003cbr\u003e \u003cbr\u003e Experiment 13 Analysis of Sequencing Data\u003cbr\u003e Verification of Insert Sequence\u003cbr\u003e \u003cbr\u003e Experiment 14 Gene Expression\u003cbr\u003e Transformation into Expression Host\u003cbr\u003e \u003cbr\u003e Experiment 15 Induction of LTB Gene Expression\u003cbr\u003e \u003cbr\u003e Part 2 Protein Chemistry\u003cbr\u003e \u003cbr\u003e Experiment 16 SDS-PAGE of Induction Time Course\u003cbr\u003e Transfer of Protein to Membrane for Western Blot\u003cbr\u003e \u003cbr\u003e Experiment 17 Visualization of Western Blot\u003cbr\u003e \u003cbr\u003e Experiment 18 Large Scale Culture Preparation\u003cbr\u003e \u003cbr\u003e Experiment 19 Isolation of LTB by Affinity Chromatography\u003cbr\u003e \u003cbr\u003e Experiment 20 SDS-PAGE of Column Fractions\u003cbr\u003e Immunoblot to verify Presence of LTB\u003cbr\u003e \u003cbr\u003e Experiment 21 Protein Concentration\u003cbr\u003e Protein Crystallization\u003cbr\u003e \u003cbr\u003e Experiment 22 Setting up an ELISA Microtiter Plate\u003cbr\u003e \u003cbr\u003e Experiment 23 ELISA of a Panel of Ganglioside Ligands\u003cbr\u003e \u003cbr\u003e Experiment 24 Protein Structure Determination: X-ray Diffraction Techniques\u003cbr\u003e \u003cbr\u003e Experiment 25 Characterization of a Protein Crystal\u003cbr\u003e \u003cbr\u003e Experiment 26 Primer Design","brand":"John Wiley \u0026 Sons Inc","offers":[{"title":"Default Title","offer_id":49402435535191,"sku":"9780470843604","price":111.56,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9780470843604.jpg?v=1730480388","url":"https:\/\/bookcurl.com\/products\/methods-in-molecular-biology-protein-chemistry-cloning-and-characterization-of-an-enterotoxin-subunit-9780470843604","provider":"Book Curl","version":"1.0","type":"link"}